The lambda - P22 problem

Ackermann, Hans-W.

doi:10.1080/21597081.2015.1017084

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…Fig. 5d, similarity between NCVicG45 siphoviruses with the singleton NCVicG25 podovirus), paralleling the observation that the podovirus P22 shares sequence similarity to lambdoid siphoviruses in its non-structural genes 40 ; other pairs share only a single 25-mer, for example in a DNA polymerase gene (e.g. in protein cluster mmseq 2145 in podovirus 1.262.O Fig.…”

Section: Resultssupporting

confidence: 56%

Resolving the structure of phage–bacteria interactions in the context of natural diversity

et al. 2022

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Microbial communities are shaped by viral predators. Yet, resolving which viruses (phages) and bacteria are interacting is a major challenge in the context of natural levels of microbial diversity. Thus, fundamental features of how phage-bacteria interactions are structured and evolve in the wild remain poorly resolved. Here we use large-scale isolation of environmental marine Vibrio bacteria and their phages to obtain estimates of strain-level phage predator loads, and use all-by-all host range assays to discover how phage and host genomic diversity shape interactions. We show that lytic interactions in environmental interaction networks (as observed in agar overlay) are sparse—with phage predator loads being low for most bacterial strains, and phages being host-strain-specific. Paradoxically, we also find that although overlap in killing is generally rare between tailed phages, recombination is common. Together, these results suggest that recombination during cryptic co-infections is an important mode of phage evolution in microbial communities. In the development of phages for bioengineering and therapeutics it is important to consider that nucleic acids of introduced phages may spread into local phage populations through recombination, and that the likelihood of transfer is not predictable based on lytic host range.

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Section: Resultssupporting

confidence: 56%

Resolving the structure of phage–bacteria interactions in the context of natural diversity

et al. 2022

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“…In addition, a synthetic approach is expected to be used to unveil the biological properties of phages that have not been well elucidated. Although the analysis of model phages, such as the so-called T series, λ, MS2, Qβ, ΦX174, Mu, Φ29, P1, P2, and P22, has made significant progress to some extent [ 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 ], considering a massive amount of phages in nature, which is about 10 31 , many are still awaiting discovery. Moreover, Jumbo phages, which have large genome sizes, have been discovered, but most of their genetic properties remain unknown [ 103 , 104 , 105 ].…”

Section: Future Directionmentioning

confidence: 99%

Bacteriophage Technology and Modern Medicine

et al. 2021

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The bacteriophage (or phage for short) has been used as an antibacterial agent for over a century but was abandoned in most countries after the discovery and broad use of antibiotics. The worldwide emergence and high prevalence of antimicrobial-resistant (AMR) bacteria have led to a revival of interest in the long-forgotten antibacterial therapy with phages (phage therapy) as an alternative approach to combatting AMR bacteria. The rapid progress recently made in molecular biology and genetic engineering has accelerated the generation of phage-related products with superior therapeutic potentials against bacterial infection. Nowadays, phage-based technology has been developed for many purposes, including those beyond the framework of antibacterial treatment, such as to suppress viruses by phages, gene therapy, vaccine development, etc. Here, we highlighted the current progress in phage engineering technology and its application in modern medicine.

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