Abstract.A craquelure structure of protein film as indicator of macromolecule state is discussing. Craquelure is a network of fine cracks or crackles on the surface of a painting, caused chiefly by shrinkage of paint film or varnish. The actions of laser radiation in the red and green spectral region on the protein film craquelure structure by the example of albumin are considering. It is experimentally shown that after drying the protein layer a craquelure pattern (variety of cracks in the layer) is formed with the parameters strongly modified by the laser action and depending on the wavelength of radiation and the time (energy density) of exposure.