This study examines the scope and determinants of serial entrepreneurship among employer firms of Latino-owned businesses (LOBs) in the USA. The primary data are derived from the 2023 Stanford Latino Entrepreneurship Initiative nationally representative survey of 5102 LOBs and Latino entrepreneurs. The scope of Latino serial entrepreneurship—entrepreneurs who have started more than one business—reveals approximately 30% of Latino entrepreneurs have started two or more enterprises. The determinants of serial entrepreneurship are derived from entrepreneur demographics, utilizing binomial logistic regression to predict the odds of Latino serial entrepreneurship. Findings suggest Latino serial entrepreneurs are more likely to be male, native US-born, identify less as Latino, reside in the Midwest and South, possess high levels of education, and are of Mexican descent.