The level of a relatively purine-specific ribonuclease increases in virus-infected hypersensitive or mechanically injured tobacco leaves

Wyen, N.V.; Udvardy, J.; Erdei, Sára; Farkas, G. L.

doi:10.1016/0042-6822(72)90044-x

Cited by 30 publications

(15 citation statements)

References 13 publications

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“…However, the results obtained corroborate and extend the findings of Wyen et al [5] about the presence of different types of nuclease activity in tobacco leaves. Four major and probably three minor enzyme bands fall into the class of (endo) ribonuclease; two major and eight minor bands show nuclease activity; three of these can be classified as acid phosphodiesterases.…”

Section: Discussionsupporting

confidence: 91%

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Polynucleotide‐acrylamide gel electrophoresis of soluble nucleases from tobacco leaves

Loon

1975

FEBS Letters

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Section: Discussionsupporting

confidence: 91%

“…10 and the stronger activity observed in this area in poly-d(A-T) control gels. The four major ribonuclease isoenzymes were found to be charge isomers, as was expected from the determination of a single molecular weight for this enzyme in tobacco leaves [ 5,6] .…”

Section: Discussionsupporting

confidence: 66%

Polynucleotide‐acrylamide gel electrophoresis of soluble nucleases from tobacco leaves

Loon

1975

FEBS Letters

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“…It was reported that RNase activities were increased in reponse to rust infection in wheat [3,6] or in response to tobacco mosaic virus infection in tobacco [39]. It was proposed that such an increase of RNase activity might be involved in the defense of the plant [3 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of cDNAs encoding xylogenesis-associated and wounding-induced ribonucleases in Zinnia elegans

Droste

1996

Plant Mol Biol

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The study of plant ribonuclease (RNase) functions is complicated by a complex profile of RNase activities detected in tissues. Thus, isolation of individual RNase genes will be desirable for the further understanding of function of each RNase. Here, we describe the isolation of cDNAs encoding two RNases, ZRNaseI and ZRNaseII, in differentiating tracheary elements (TEs) induced from isolated mesophyll cells of Zinnia elegans. Both the ZRNaseI and ZRNaseII exhibit putative secretion signal sequences at the amino-terminal ends with predicted molecular masses of 24 247 Da and 22 448 Da as mature proteins, respectively. DNA gel blot analysis showed that both RNases in Zinnia appear to be encoded by a small gene family. RNA gel blot analysis showed that the expression of the ZRNaseI gene was associated with the late stage of in vitro TE differentiation, whereas the ZRNaseII gene was mainly induced in response to stress. Neither RNase gene was induced in response to phosphate starvation, or to H2O2 challenge in the cultured mesophyll cells, or to senescence in the leaves. In young leaves, the ZRNaseI gene was not induced in response to wounding. But the ZRNaseII gene was markedly induced by 6 h after wounding. Tissue print hybridization showed that the expression of the ZRNaseI gene was preferentially associated with the differentiation TEs in Zinnia stems, while the ZRNaseII mRNA was not detected in unwounded Zinnia organs. Taken together, the results indicated that the ZRNaseI gene is expressed during the process of xylogenesis both in vitro and in the plant, whereas the ZRNaseII gene is predominantly induced in response to wounding. The identification of these RNase genes provides molecular tools for the dissection of the process of autolysis during xylogenesis, and for the dissection of the role of RNase in wounding response.

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“…Diener (1961) observed stimulation of RNases in TMV-inoculated Datura stramonium and in BPMV-inoculated Phaseolus vulgaris, Wyen et al (1972) found increased concentration of a relatively purine specific endoribonuclease in TMV-inoculated Xanthi-nc tobacco leaves, and Randles (1968) reported that the activity of one of three host RNases rose significantly at the time of rapid virus accumulation in Chinese cabbage systemically infected with TYMV. The similar results were found also by Reddi (1959Reddi ( , 1963, Cheo (1971), Šindelář et al (1990), Šindelářová et al (2000, 2002), Šindelářová and Šindelář (2003, 2003/4, 2004).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 97%

Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: Host's RNA degradation

Šindelář¹,

Šindelářová²

2005

Biologia plant.

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Tobacco plants infected with the potato virus Y (PVY) were studied during the acute-infection period. The control enzymes of metabolic pathway of host´s RNA degradation tending to biosynthesis of PVY-RNA, its coarse/fine regulation and content of host´s RNA were monitored. Activities of ribonucleases, phosphomonoesterases and phosphodiesterases in both the crude homogenates and the partially purified enzyme preparations from the diseased leaves were markedly increased when compared to the tissues from healthy plants. The curves of enzyme activities positively correlated with the multiplication curve of the PVY and negatively correlated with the decreased contents of host´s RNA. The enzyme activity in homogenate samples did not significantly differ from the corresponding purified enzyme preparations.

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The level of a relatively purine-specific ribonuclease increases in virus-infected hypersensitive or mechanically injured tobacco leaves

Cited by 30 publications

References 13 publications

Polynucleotide‐acrylamide gel electrophoresis of soluble nucleases from tobacco leaves

Polynucleotide‐acrylamide gel electrophoresis of soluble nucleases from tobacco leaves

Isolation and characterization of cDNAs encoding xylogenesis-associated and wounding-induced ribonucleases in Zinnia elegans

Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: Host's RNA degradation

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