The levels of PCV2 specific antibodies and viremia in pigs

Carasová, P.; Celer, V.; Takacova, K.; Trundová, Mária; Molínková, Dobromila; Lobová, Dana; Smoła, J.

doi:10.1016/j.rvsc.2006.11.013

Cited by 37 publications

(23 citation statements)

References 21 publications

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“…1 and 3). Similar dynamics of viraemia and antibody response in non-vaccinated piglets after natural PCV-2 infection were observed previously by Carasova et al (19). As in our study, the animals remained viraemic up to the end of the investigation period, even in the presence of post-infectious antibodies.…”

Section: Resultssupporting

confidence: 91%

Dynamics of antibody response and viraemia following natural infection of porcine circovirus 2 (PCV‐2) in a conventional pig herd

Csank¹,

Pistl²,

Polláková³

et al. 2013

APMIS

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Porcine circovirus 2 (PCV-2) is a primary agent of post-weaning multi-systemic wasting syndrome (PMWS), ubiquitous in pig herds. The course of viraemia and seroconversion in naturally infected pigs were investigated in piglets from the 2nd week of their life. Piglets were divided into seropositive (Ab(+)) and seronegative (Ab(-)) groups. Subsequently, after vaccination against PCV-2 (Ingelvac(®) CIRCOFLEX™, Böehringer Ingelheim), they were further divided into non-vaccinated seronegative (NVAC/Ab(-)) and seropositive (NVAC/Ab(+)), and vaccinated seronegative (VAC/Ab(-)) and seropositive (VAC/Ab(+)). PCV-2 colostral antibodies failed to prevent development of natural PCV-2 infection in conventional piglets; however, this occurred at a higher age in comparison with seronegative pigs. Neither colostral nor post-infection antibodies prevented development of viraemia, which persisted up to the end of the study (the 19th week), but without clinical signs of PMWS. Vaccination failed to prevent development of natural PCV-2 infection, but viraemia was limited to between the 8th and 10th week. The presence of colostral anti-PCV-2 antibodies did not show any untoward effect to vaccination; on the contrary, VAC/Ab(+) animals showed the lowest titre of viraemia.

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Section: Resultssupporting

confidence: 91%

Dynamics of antibody response and viraemia following natural infection of porcine circovirus 2 (PCV‐2) in a conventional pig herd

Csank¹,

Pistl²,

Polláková³

et al. 2013

APMIS

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“…This observation is similar to a previous report that seroconversion on PMWS-affected farms appeared earlier than on non-PMWS-affected farms (4). The double-antigen sandwich ELISA indicates similar serological profiles for the 4 farms, which were consistent with the profiles obtained by the reference commercial indirect ELISA and also in agreement with results of previous reports (5,14,25).…”

Section: Discussionsupporting

confidence: 91%

Development and Application of a Double-Antigen Sandwich Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Porcine Circovirus 2

Luo

Jiang

et al. 2012

Clin Vaccine Immunol

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ABSTRACTA double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) is described for detection of porcine circovirus 2 (PCV2) antibodies using the well-characterized recombinant PCV2 capsid protein. In a comparative test of 394 pig sera against an indirect immunofluorescence (IIF) test and a commercial ELISA kit (also based on the recombinant PCV2 capsid protein), the results showed that the diagnostic sensitivity, specificity, and accuracy of the assay were, respectively, 90.61, 94.02, and 91.62% compared with IIF and 94.38, 95.28, and 94.67% compared with the commercial ELISA kit. Assay of 12 PCV-free pigs over a 5-week period produced only PCV2-negative titers by all 3 methods. These results and the seroprofiles of 4 pig farms obtained by both the commercial ELISA kit and the double-antigen sandwich ELISA indicate that the sandwich ELISA is a reliable method for detection of antibodies to PCV2. Additionally, the method described here permits the use of undiluted test serum samples simultaneously loaded with horseradish peroxidase (HRP)-conjugated antigen into the test well, and the complete test procedure can be performed in less than 90 min. This double-antigen sandwich ELISA should be a useful tool to aid swine industry professionals in deciding the intervention strategies for the control of PCV2-associated diseases.

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“…The observed serological responses to PCV2 are well in line with previous studies [12,17,19-21] supporting the relationship between PCV2 and PMWS also in finishing pigs. The lack of a proper antibody response in the two pigs that developed PMWS in herd A, further support earlier studies pointing out that neutralizing antibodies to PCV2 are protective against PMWS [22-25].…”

Section: Discussionsupporting

confidence: 91%

Dynamics of serum antibodies to and load of porcine circovirus type 2 (PCV2) in pigs in three finishing herds, affected or not by postweaning multisystemic wasting syndrome

et al. 2010

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BackgroundDespite that PMWS commonly affects pigs aged eight to sixteen weeks; most studies of PMWS have been conducted during the period before transfer to finishing herds. This study focused on PCV2 load and antibody dynamics in finishing herds with different PMWS status.MethodsSequentially collected blood samples from 40 pigs in each of two Swedish (A and B) and one Norwegian (C) finishing herds were analysed for serum PCV2-load and -antibodies and saliva cortisol. The two Swedish herds differed in PMWS status, despite receiving animals from the same sow pool (multi-site production). However, the PMWS-deemed herd (A) had previously also received pigs from the spot market. ResultsThe initial serum PCV2 load was similar in the two Swedish herds. In herd A, it peaked after two weeks in the finishing herd and a high number of the pigs had serum PCV2 levels above 107 per ml. The antibody titres increased continually with exception for the pigs that developed PMWS, that had initially low and then declining antibody levels. Pigs in the healthy herd B also expressed high titres of antibodies to PCV2 on arrival but remained at that level throughout the study whereas the viral load steadily decreased. No PCV2 antibodies and only low amounts of PCV2 DNA were detected in serum collected during the first five weeks in the PMWS-free herd C. Thereafter a peak in serum PCV2 load accompanied by an antibody response was recorded. PCV2 from the two Swedish herds grouped into genotype PCV2b whereas the Norwegian isolate grouped into PCV2a. Cortisol levels were lower in herd C than in herds A and B.ConclusionsThe most obvious difference between the Swedish finishing herds and the Norwegian herd was the time of infection with PCV2 in relation to the time of allocation, as well as the genotype of PCV2. Clinical PMWS was preceded by low levels of serum antibodies and a high load of PCV2 but did not develop in all such animals. It is notable that herd A became affected by PMWS after errors in management routine, emphasising the importance of proper hygiene and general disease-preventing measures.

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The levels of PCV2 specific antibodies and viremia in pigs

Cited by 37 publications

References 21 publications

Dynamics of antibody response and viraemia following natural infection of porcine circovirus 2 (PCV‐2) in a conventional pig herd

Dynamics of antibody response and viraemia following natural infection of porcine circovirus 2 (PCV‐2) in a conventional pig herd

Development and Application of a Double-Antigen Sandwich Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Porcine Circovirus 2

Dynamics of serum antibodies to and load of porcine circovirus type 2 (PCV2) in pigs in three finishing herds, affected or not by postweaning multisystemic wasting syndrome

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