The Lewis A phenotype is a restriction factor for Rotateq and Rotarix vaccine-take in Nicaraguan children

Bucardo, Filemón; Nordgren, Johan; Reyes, Yaoska; González, Fredman; Sharma, Sumit; Svensson, Lennart

doi:10.1038/s41598-018-19718-y

Cited by 62 publications

(63 citation statements)

References 45 publications

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“…As genotyping was not possible in this study, and weak secretors have a similar susceptibility pattern to norovirus as non-secretors (Liu et al 2014), these samples were classified as non-secretors due to the negativity for α1,2 fucose. Previous studies have observed a strong correlation by non-secretor genotype and non-secretor phenotype by the UEA-1 agglutinin assay (Bucardo et al 2018). Another limitation is that only five out of the eight available feces samples were successfully genotyped and the norovirus-positive coleslaw could not be genotyped.…”

Section: Discussionmentioning

confidence: 96%

Secretor Status is Associated with Susceptibility to Disease in a Large GII.6 Norovirus Foodborne Outbreak

et al. 2019

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Norovirus is commonly associated with food and waterborne outbreaks. Genetic susceptibility to norovirus is largely dependent on presence of histo-blood group antigens (HBGA), specifically ABO, secretor, and Lewis phenotypes. The aim of the study was to determine the association between HBGAs to norovirus susceptibility during a large norovirus foodborne outbreak linked to genotype GII.6 in an office-based company in Stockholm, Sweden, 2015. A two-episode outbreak with symptoms of diarrhea and vomiting occurred in 2015. An online questionnaire was sent to all 1109 employees that had worked during the first outbreak episode. Food and water samples were collected from in-house restaurant and tested for bacterial and viral pathogens. In addition, fecal samples were collected from 8 employees that had diarrhea. To investigate genetic susceptibility during the outbreak, 98 saliva samples were analyzed for ABO, secretor, and Lewis phenotypes using ELISA. A total of 542 of 1109 (49%) employees reported gastrointestinal symptoms. All 8 fecal samples tested positive for GII norovirus, which was also detected in coleslaw collected from the in-house restaurant. Eating at the in-house restaurant was significantly associated with risk of symptom development. Nucleotide sequencing was successful for 5/8 fecal samples and all belonged to the GII.6 genotype. HBGA characterization showed a strong secretor association to norovirus-related symptoms (P = 0.014). No association between norovirus disease and ABO phenotypes was observed. The result of this study shows that non-secretors were significantly less likely to report symptoms in a large foodborne outbreak linked to the emerging GII.6 norovirus strain.

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Section: Discussionmentioning

confidence: 96%

Secretor Status is Associated with Susceptibility to Disease in a Large GII.6 Norovirus Foodborne Outbreak

et al. 2019

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“…The Manguinhos cohort showed a high prevalence of secretors, being a common profile in Latin America 30,31,44,45,49 . In this study, we were able to investigate RVA shedding and a common mutation was identified in many samples characterized as RV1 according to VP8* Sanger sequencing criteria.…”

Section: Discussionmentioning

confidence: 99%

Rotavirus A shedding and HBGA host genetic susceptibility in a birth community-cohort, Rio de Janeiro, Brazil, 2014–2018

Cantelli

Velloso²,

Assis

et al. 2020

Sci Rep

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Recent studies have investigated whether the human histo-blood group antigen (HBGAs) could affect the effectiveness of the oral rotavirus vaccines, suggesting secretor positive individuals develop a more robust response. We investigated the Rotavirus A (RVA) shedding in association with the host susceptibility profile in children from a birth community-cohort in Rio de Janeiro, Brazil, from 2014 to 2018. A total of 132 children were followed-up between 0 to 11-month-old, stool samples were collected before/after the 1 st /2 nd RV1 vaccination doses and saliva samples were collected during the study. RVA shedding was screened by RT-qPCR and G/P genotypes determined by multiplex RT-PCR and/or Sanger nucleotide sequencing. The sequencing indicated an F167L amino acid change in the RV1 VP8* P[8] in 20.5% of shedding follow-ups and these mutant subpopulations were quantified by pyrosequencing. The HBGA/secretor status was determined and 80.3% of the children were secretors. Twenty-one FUT2 gene SNPs were identified and two new mutations were observed. The mutant F167L RV1 VP8* P[8] was detected significantly more in Le (a+b+) secretors (90.5%) compared to non-secretors and even to secretors Le (a−b+) (9.5%). The study highlights the probable association between RV1 shedding and HBGAs as a marker for evaluating vaccine strain host susceptibility. After implementation of the rotavirus vaccines, Rotarix ® (RV1, GlaxoSmithKline Biologicals, Rixensart, Belgium) and RotaTeq ® (RV5, Merck Inc., USA), in over 100 countries worldwide 1 , the burden of severe group A rotaviruses (RVA) diarrhea has decreased substantially, with reductions in hospitalizations and deaths in many countries, including Brazil 2-6. However, RVA still are one of major causes of severe viral diarrhea in infants and young children <5-years-old worldwide 6,7. Due to the zoonotic potential, variability of the RVA strains and host genetic factors, the surveillance of circulating RVA strains is necessary for evaluating and monitoring the effectiveness of the implemented immunization programs, mainly in low-income countries 8. Currently, 36 G-and 51 P-genotypes have been described 9 and globally, six G/P combinations are the most prevalent in humans: G1P[8], G2P[4], G3P[8], G9P[8], G4P[8] and G12P[8] 10 .

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“…The number of Lewis-negative non-secretors was too low to make a statistical comparison. The same study also assessed RotaTeq and found the same results, i.e., no seroconversion for Lewis a phenotype children, but the numbers were too low for reliable interpretation [53]. Studies from Malawi [15] and Nicaragua [27] have also measured vaccine shedding after vaccination, as determined ≥4 days post-vaccination, to measure active vaccine strain replication.…”

Section: Rotavirus Vaccine Take Is Associated With Hbgasmentioning

confidence: 95%

“…Studies from Nicaragua [27,53], Pakistan [54], Ghana [55], and Malawi [15] have tested this hypothesis by measuring IgA seroconversion and/or vaccine strain shedding in association to HBGAs (Table 3). Five studies investigated Rotarix whereas one study investigated the RotaTeq vaccine.…”

Section: Rotavirus Vaccine Take Is Associated With Hbgasmentioning

confidence: 99%

The Impact of Human Genetic Polymorphisms on Rotavirus Susceptibility, Epidemiology, and Vaccine Take

Sharma

Hagbom

Svensson

et al. 2020

Viruses

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Innate resistance to viral infections can be attributed to mutations in genes involved in the immune response, or to the receptor/ligand. A remarkable example of the latter is the recently described Mendelian trait resistance to clinically important and globally predominating genotypes of rotavirus, the most common agent of severe dehydrating gastroenteritis in children worldwide. This resistance appears to be rotavirus genotype-dependent and is mainly mediated by histo-blood group antigens (HBGAs), which function as a receptor or attachment factors on gut epithelial surfaces. HBGA synthesis is mediated by fucosyltransferases and glycosyltransferases under the genetic control of the FUT2 (secretor), FUT3 (Lewis), and ABO (H) genes on chromosome 19. Significant genotypic and phenotypic diversity of HBGA expression exists between different human populations. This genetic diversity has an effect on genotype-specific susceptibility, molecular epidemiology, and vaccine take. Here, we will discuss studies on genetic susceptibility to rotavirus infection and place them in the context of population susceptibility, rotavirus epidemiology, vaccine take, and public health impact.

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The Lewis A phenotype is a restriction factor for Rotateq and Rotarix vaccine-take in Nicaraguan children

Cited by 62 publications

References 45 publications

Secretor Status is Associated with Susceptibility to Disease in a Large GII.6 Norovirus Foodborne Outbreak

Secretor Status is Associated with Susceptibility to Disease in a Large GII.6 Norovirus Foodborne Outbreak

Rotavirus A shedding and HBGA host genetic susceptibility in a birth community-cohort, Rio de Janeiro, Brazil, 2014–2018

The Impact of Human Genetic Polymorphisms on Rotavirus Susceptibility, Epidemiology, and Vaccine Take

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