The Limitations of Pulsed‐Field Gel Electrophoresis for Analysis of <i><scp>Y</scp>ersinia enterocolitica</i> Isolates

Gilpin, Brent; Robson, Beth; Lin, Shen; Hudson, John; Weaver, Louise; Dufour, Muriel; Strydom, Hugo

doi:10.1111/zph.12085

Cited by 22 publications

(24 citation statements)

References 27 publications

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“…Although the number of biotype 2 strains was 46 and closer to our sampling, the discrimination index was 0·319 considering just strains of this biotype (Gilpin et al . ) and in this present study the discrimination index was 0·965 (data not shown). In this way, we may conclude that the use of Xba I in PFGE is more suitable to genotype and differentiate Y .…”

Section: Discussionsupporting

confidence: 52%

“…Also, Gilpin et al . () used the restriction enzymes Apa I and Not I. Although the number of biotype 2 strains was 46 and closer to our sampling, the discrimination index was 0·319 considering just strains of this biotype (Gilpin et al .…”

Section: Discussionmentioning

confidence: 82%

“…() found a lower discrimination index of 0·862 and Gilpin et al . () of 0·844. This discrepancy may be due to the sampling studied and the restriction enzymes used in the studies mentioned above.…”

Section: Discussionmentioning

confidence: 99%

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Genotypic diversity and pathogenic potential ofYersinia enterocoliticabiotype 2 strains isolated in Brazil

Frazão¹,

Falcão²

2015

J Appl Microbiol

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 82%

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Genotypic diversity and pathogenic potential ofYersinia enterocoliticabiotype 2 strains isolated in Brazil

Frazão¹,

Falcão²

2015

J Appl Microbiol

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“…enterocolitica [49], the strains were then subjected to MLVA, which has a higher discriminatory power [36,37]. This MLVA analysis based on six loci also gave a unique pattern (7-8-7-8-8-2) for the 14 Y .…”

Section: Resultsmentioning

confidence: 99%

Yersinia enterocolitica, a Neglected Cause of Human Enteric Infections in Côte d’Ivoire

et al. 2017

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BackgroundEnteropathogenic Yersinia circulate in the pig reservoir and are the third bacterial cause of human gastrointestinal infections in Europe. In West Africa, reports of human yersiniosis are rare. This study was conducted to determine whether pathogenic Yersinia are circulating in pig farms and are responsible for human infections in the Abidjan District.Methodology/Principal findingsFrom June 2012 to December 2013, pig feces were collected monthly in 41 swine farms of the Abidjan district. Of the 781 samples collected, 19 Yersinia strains were isolated in 3 farms: 7 non-pathogenic Yersinia intermedia and 12 pathogenic Yersinia enterocolitica bioserotype 4/O:3. Farm animals other than pigs and wild animals were not found infected. Furthermore, 2 Y. enterocolitica 4/O:3 strains were isolated from 426 fecal samples of patients with digestive disorders. All 14 Y. enterocolitica strains shared the same PFGE and MLVA profile, indicating their close genetic relationship. However, while 6 of them displayed the usual phage type VIII, the other 8 had the highly infrequent phage type XI. Whole genome sequencing and SNP analysis of individual colonies revealed that phage type XI strains had unusually high rates of mutations. These strains displayed a hypermutator phenotype that was attributable to a large deletion in the mutS gene involved in DNA mismatch repair.Conclusions/SignificanceThis study demonstrates that pathogenic Y. enterocolitica circulate in the pig reservoir in Côte d'Ivoire and cause human infections with a prevalence comparable to that of many developed countries. The paucity of reports of yersiniosis in West Africa is most likely attributable to a lack of active detection rather than to an absence of the microorganism. The identification of hypermutator strains in pigs and humans is of concern as these strains can rapidly acquire selective advantages that may increase their fitness, pathogenicity or resistance to commonly used treatments.

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“…Many studies, for example, have used NotI and XbaI as restriction enzymes (Bonardi et al, ; Falcão, Falcão, Pitondo‐Silva, Malaspina, & Brocchi, ; Fredriksson‐Ahomaa, Stolle, & Stephan, ). However, limited diversity among biotypes 4 and 3 was observed at pig slaughterhouse level even between strains of different geographical origins, like Germany, Finland, New Zealand, and China (Fredriksson‐Ahomaa et al, ; Gilpin et al, ; Liang et al, ). Another increasingly popular technique used to type Y. enterocolitica is the Multi‐locus variable number tandem repeat analysis (MLVA).…”

Section: Introductionmentioning

confidence: 99%

Diversity of Yersinia enterocolitica isolated from pigs in a French slaughterhouse over 2 years

et al. 2018

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The pig is one of the main reservoirs of Yersinia enterocolitica strains pathogenic to humans. A description of the Y. enterocolitica population in this reservoir, and accurate discriminatory techniques for typing isolates are needed for prevention, outbreak investigation, and surveillance. This study investigates the genetic diversity of pathogenic Y. enterocolitica isolates obtained from pig tonsils in a French pig slaughterhouse in 2009 (S1) and 2010 (S2). The use of Pulsed‐Field Gel Electrophoresis (PFGE) and MLVA as typing techniques was also compared and evaluated. First, a total of 167 isolates (12 of biotype 3 recovered during S1, and 155 of biotype 4 recovered during S1 and S2) were typed by PFGE using the XbaI enzyme. MLVA was then tested on all the biotype 3 isolates in addition to 70 selected biotype 4 isolates recovered over the 2 years. PFGE generated two specific XbaI‐PFGE profiles for biotype 3 isolates. Nine XbaI profiles were obtained for biotype 4, with a higher diversity (ID = 0.599) than biotype 3 (ID = 0.167). Two out of the nine XbaI profiles were reported during both surveys and at different months. MLVA improved the differentiation between isolates; the index of diversity reached 0.621 and 0.958, respectively, for biotype 3 (three MLVA types) and biotype 4 (32 MLVA types). The MLVA types for biotype 4 differed over the two surveys, but some isolates with different MLVA types were genetically closely related. This study provides an initial evaluation of the genetic diversity of Y. enterocolitica strains isolated from pigs in France. We show that some PFGE profiles are maintained in the pig production sector, and, through MLVA, that part of the Y. enterocolitica population remained genetically close over the two years. MLVA proved its effectiveness as a tool for investigating pathogenic Y. enterocolitica strains isolated from pigs.

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The Limitations of Pulsed‐Field Gel Electrophoresis for Analysis of Yersinia enterocolitica Isolates

Cited by 22 publications

References 27 publications

Genotypic diversity and pathogenic potential ofYersinia enterocoliticabiotype 2 strains isolated in Brazil

Genotypic diversity and pathogenic potential ofYersinia enterocoliticabiotype 2 strains isolated in Brazil

Yersinia enterocolitica, a Neglected Cause of Human Enteric Infections in Côte d’Ivoire

Diversity of Yersinia enterocolitica isolated from pigs in a French slaughterhouse over 2 years

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