The localization of CD3, CD79a, CD68 and S100 protein immunoreactive cells in hemal nodes of Saanen goat (Capra hircus)

Abstract

We investigated the structure of hemal nodes in Saanen goats using immunohistochemical staining. We examined the distribution of CD3 positive T lymphocytes, CD79a positive B lymphocytes, CD68 positive macrophages and S100 protein positive follicular dendritic cells. Hemal nodes of six healty adult female goats were used. Hemal nodes were removed from the thoracic and abdominal cavities. The oval to round hemal nodes were observed especially between the abdominal aorta and vena cava, and near the kidneys and adrenal glands. Tissue sections were stained with Crossmon's modified triple stain to demonstrate general histological structure. The avidin-biotin-peroxidase technique using anti-CD3, anti-CD79a, anti-CD68 and anti-S100 primary antibodies was used for immunohistochemistry. Many CD3 positive T lymphocytes were found in the germinal center of the lymph follicles and in the lymphatic cords of hemal nodes; CD3 positive cells also were observed in the sinuses. CD79a and CD68 positive cells were found at the germinal center of the lymph follicles. In the lymph follicles near the subcapsular sinuses, CD79a and CD68 positive cells were found especially in e areas bordering the mantle zone. S100 positive cells were found in the lymph follicles, lymphatic cords and sinuses.

Zoological terms in the human histological nomenclature Terminologia Histologica. What we think, what we know, and what we think we know

“…They are capable of performing haematopoietic and immunological functions. These lymphoid organs were described in detail in water buffalos (Zidan and Pabst 2010), cattle (Cerutti et al 1998;Zhang et al 2013), goats (Bozkurt et al 2018), dromedary camels (Zidan and Pabst 2004), pigs (Doboszyńska 2002;Akaydin and Kabak 2006), domestic yaks (Huang et al 2019), roe deer (Akaydın Bozkurt et al 2018) or rats (Castenholz and Castenholz 1996). In spite of that, these organs are in general not known to many immunologists, they are not mentioned in most immunological textbooks, and their presence in humans is controversial until today.…”

“…Haemal lymph nodes and haemal nodes are dark to brown-red, small, bean-shaped or spherical encapsulated organs (2-12 mm in diameter), typically embedded in adipose tissue (Zidan and Pabst 2004). Anatomically they are localized alongside the inferior vena cava and abdominal aorta, near lymph nodes, and especially around blood vessels in the mediastinum, abdominal cavity, mesentery, and pelvic cavity (Akaydın Bozkurt et al 2018). Goat haemal nodes house a large population of T-lymphocytes, B-lymphocytes, macrophages and S100-protein positive (probably dendritic) cells (Bozkurt et al 2018).…”

“…Anatomically they are localized alongside the inferior vena cava and abdominal aorta, near lymph nodes, and especially around blood vessels in the mediastinum, abdominal cavity, mesentery, and pelvic cavity (Akaydın Bozkurt et al 2018). Goat haemal nodes house a large population of T-lymphocytes, B-lymphocytes, macrophages and S100-protein positive (probably dendritic) cells (Bozkurt et al 2018).…”