The long non-coding RNA BC200 (BCYRN1) is critical for cancer cell survival and proliferation

Booy, Evan P.; McRae, Ewan K.S.; Koul, Amit; Lin, Francis; McKenna, Sean Andrew

doi:10.1186/s12943-017-0679-7

Cited by 79 publications

(88 citation statements)

References 31 publications

(72 reference statements)

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“…It is universally expressed in the nervous system, as well as in several cancers . BC200 expression is elevated in proliferating cultured cells, making it a promising therapeutic target for a broad spectrum of cancers . Our results showed a significant upregulation of BC200 by EBV, revealing, for the first time, the relationship between BC200 and EBV.…”

Section: Discussionsupporting

confidence: 50%

Differential expression profiling of lncRNAs related to Epstein‐Barr virus infection in the epithelial cells

Zhang

Zuo

et al. 2019

Journal of Medical Virology

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Epstein‐Barr virus (EBV) is a prevalent γ‐herpesvirus associated with a variety of cancers, including epithelial nasopharyngeal carcinoma (NPC) and gastric carcinoma (GC). Long noncoding RNAs (lncRNAs) are emerging as powerful regulators that have demonstrated to play crucial roles in cancer development. In this approach, based on genome‐wide RNA sequencing, we examined the lncRNA expression profiles in four EBV genome‐infected and EBV‐negative 293 cells. A series of lncRNAs were found to be dysregulated in a comparative analysis. Then, eight typical lncRNAs were selected for validation of expression levels by real‐time quantitative polymerase chain reaction and were detected in EBV‐positive or EBV‐negative GC and NPC cells. The differential expression patterns of the eight lncRNAs for validation were fundamentally identical to those revealed in RNA sequencing. Particularly, the differential expression of these lncRNAs in GC and NPC cells indicated their possible roles in EBV infection and tumorigenesis. In addition, a predicted lncRNA‐microRNA‐messenger RNA network suggested their potential interactions. This study reveals the first lncRNAome related to EBV infection in the epithelial cells and provides novel clues for the study of viral role in epithelial cancers through the interaction between EBV and host lncRNAs.

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Section: Discussionsupporting

confidence: 50%

Differential expression profiling of lncRNAs related to Epstein‐Barr virus infection in the epithelial cells

Zhang

Zuo

et al. 2019

Journal of Medical Virology

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“…Recently, it has been reported that abnormal expression of BCYRN1 has an impact on varieties of human cancers [19,24,25]. However, the regulatory mechanism of BCYRN1 is still unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Dysregulation of lncRNA BCYRN1 has been found in various cancers, as well as in neurodegenerative diseases [18,19]. However, the clinical role of BCYRN1 and its biological functions in CRC remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Long Noncoding RNA BCYRN1 Promotes the Proliferation of Colorectal Cancer Cells via Up-Regulating NPR3 Expression

Lü

Zhou

et al. 2018

Cell Physiol Biochem

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Background/Aims: Long noncoding RNAs (lncRNAs) constitute a large proportion of noncoding transcripts that have recently emerged as a new class of important regulators in cancers. LncRNA BCYRN1, also known as BC200, has a potential function in tumorigenesis. However, the clinical significance of BCYRN1 and its effect on colorectal cancer (CRC) progression remains unclear. Methods: Quantitative reverse transcriptase PCR (qRT-PCR) was performed to investigate the expression of BCYRN1 in CRC tissues and cell lines. The biological function of BCYRN1 was also investigated through knockdown and overexpression of BCYRN1 in vitro. Microarray bioinformatics analysis was performed to analyze the putative targets of BCYRN1. Results: The results showed that BCYRN1 expression was significantly upregulated in 96 CRC tumor tissues compared with para-carcinoma control tissues. Additionally, BCYRN1 overexpression was associated with larger tumor size and advanced pathological stages in CRC patients. In vitro BCYRN1 knockdown significantly inhibited the proliferation and apoptosis of CRC cells. Furthermore, NPR3 was identified to be a target of BCYRN1 and was downregulated by BCYRN1 knockdown. Conclusion: Together, we provide the first evidence that BCYRN1 plays an oncogenic role in CRC cells. BCYRN1 may be a promising prognostic biomarker and a potential therapeutic target for CRC.

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“…Only a few studies focus on its potential molecular targets by immunohistochemistry . Recently, increasing evidences showed that carcinogenesis of various cancers are closely associated with abnormal expression of lncRNAs . So far, no study attempts to reveal the significance of lncRNA expression in EACSCC to the best of our knowledge.…”

Section: Discussionmentioning

confidence: 99%

Expression profiling of long noncoding RNA identifies lnc‐MMP3‐1 as a prognostic biomarker in external auditory canal squamous cell carcinoma

Liu

Dai

et al. 2017

Cancer Medicine

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Our previous studies suggested external auditory canal squamous cell carcinoma (EACSCC) is a rare malignancy with heterogeneous outcomes. This study aimed to identify lncRNA profile of EACSCC and determine the clinical application. Differential expression genes (DEGs) were investigated in EACSCC by whole transcriptome lncRNA arrays (GPL23178). RT‐PCR was used to quantify the microarray data. Bioinformatics analyses were performed to evaluate DEGs regulations in gene ontology and cellular pathways. Fluorescence in situ hybridization (FISH) was utilized to validate lncRNA expression. The overall survival was determined by Kaplan–Meier and log‐rank analyses. Our microarrays data had been submitted to Gene Expression Omnibus (GSE98912). We identified 5621 DEGs (3185 mRNAs, 2436 lncRNAs) in EACSCC. Lnc‐MMP3‐1 was the top one upregulated lncRNA in EACSCC with fold change of 237.2 (P < 0.001). RT‐PCR results showed similar expression levels as microarrays data. Bioinformatics analyses indicated development of EACSCC was involved in aberrant alternations of multiple biological processes and cellular pathways. FSIH assays also found lnc‐MMP3‐1 was significantly differentially overexpressed in EACSCC (P < 0.001). Tumor lnc‐MMP3‐1 levels were closely associated with differentiation degree (P = 0.016), tumor invasion (P = 0.015) and TNM stage (P = 0.015). Moreover, lnc‐MMP3‐1 expression was a significant prognostic factor in EACSCC (χ 2 = 4.276, P = 0.039). The study is the first screening and analysis of lncRNAs profile in EACSCC and provides new insights into pathogenesis of this rare disease. Our findings offered convincing evidences that lnc‐MMP3‐1 is a novel survival predictor of EACSCC patients.

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The long non-coding RNA BC200 (BCYRN1) is critical for cancer cell survival and proliferation

Cited by 79 publications

References 31 publications

Differential expression profiling of lncRNAs related to Epstein‐Barr virus infection in the epithelial cells

Differential expression profiling of lncRNAs related to Epstein‐Barr virus infection in the epithelial cells

Long Noncoding RNA BCYRN1 Promotes the Proliferation of Colorectal Cancer Cells via Up-Regulating NPR3 Expression

Expression profiling of long noncoding RNA identifies lnc‐MMP3‐1 as a prognostic biomarker in external auditory canal squamous cell carcinoma

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