The long noncoding RNA FTX promotes a malignant phenotype in bone marrow mesenchymal stem cells via the miR-186/c-Met axis

Liu, Liang; Li, Xiaojian; Shi, Yan; Chen, Hua

doi:10.1016/j.biopha.2020.110666

Cited by 13 publications

(7 citation statements)

References 33 publications

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“…Because of the functional importance of MSCs in glioma angiogenesis, they may be both origins and targets of ncRNAs for the regulation of angiogenesis. So far there are very limited studies concerning ncRNAs in MSCs (Liu L. et al, 2020 ). Future studies about the ncRNAs in regulation of MSC phenotypes or the impact of MSC-derived ncRNAs on other components in tumor vasculature will certainly inspire the development of new anti-angiogenic strategies for glioma treatment.…”

Section: Cells In the Glioma Microenvironment Involved In Ncrna-regulated Angiogenesismentioning

confidence: 99%

Non-Coding RNAs in Glioma Microenvironment and Angiogenesis

Zhang

Xia

et al. 2021

Front. Mol. Neurosci.

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Glioma, especially glioblastoma, is the most common and lethal brain tumor. In line with the complicated vascularization processes and the strong intratumoral heterogeneity, tumor-associated blood vessels in glioma are regulated by multiple types of cells through a variety of molecular mechanisms. Components of the tumor microenvironment, including tumor cells and tumor-associated stromata, produce various types of molecular mediators to regulate glioma angiogenesis. As critical regulatory molecules, non-coding RNAs (ncRNAs) inside cells or secreted to the tumor microenvironment play essential roles in glioma angiogenesis. In this review, we briefly summarize recent studies about the production, delivery, and functions of ncRNAs in the tumor microenvironment, as well as the molecular mechanisms underlying the regulation of angiogenesis by ncRNAs. We also discuss the ncRNA-based therapeutic strategies in the anti-angiogenic therapy for glioma treatment.

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Section: Cells In the Glioma Microenvironment Involved In Ncrna-regulated Angiogenesismentioning

confidence: 99%

Non-Coding RNAs in Glioma Microenvironment and Angiogenesis

Zhang

Xia

et al. 2021

Front. Mol. Neurosci.

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“…The TME is composed of various cellular components including tumor‐associated stromal and immune cells, comprehending the cytological basis for the high heterogeneity and refractoriness of gliomas 5,6 . These cells can trigger chronic inflammation and immunosuppression, hypoxia, and acidosis, and promote the development of glioma.…”

Section: Introductionmentioning

confidence: 99%

“…The TME is composed of various cellular components including tumor-associated stromal and immune cells, comprehending the cytological basis for the high heterogeneity and refractoriness of gliomas. 5,6 These cells can trigger chronic inflammation and immunosuppression, hypoxia, and acidosis, and promote the development of glioma. The macrophages in the TME, known as tumor-associated macrophages (TAMs), are the major cell subset in the stroma of gliomas, accounting for about 30-50% of total cells.…”

Section: Introductionmentioning

confidence: 99%

RNA‐binding protein DHX9 promotes glioma growth and tumor‐associated macrophages infiltration via TCF12

et al. 2022

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Background: Glioma is the most common malignant tumor of the central nervous system, with high heterogeneity, strong invasiveness, high therapeutic resistance, and poor prognosis, comprehending a serious challenge in neuro-oncology. Until now, the mechanisms underlying glioma progression have not been fully elucidated. Methods:The expression of DExH-box helicase 9 (DHX9) in tissues and cells was detected by qRT-PCR and western blot. EdU and transwell assays were conducted to assess the effect of DHX9 on proliferation, migration and invasion of glioma cells.Cocultured model was used to evaluate the role of DHX9 on macrophages recruitment and polarization. Animal study was performed to explore the role of DHX9 on macrophages recruitment and polarization in vivo. Bioinformatics analysis, dualluciferase reporter assay and chromatin immunoprecipitation (ChIP)-qPCR assay was used to explore the relation between DHX9 and TCF12/CSF1.Results: DHX9 was elevated in gliomas, especially in glioblastoma multiforme (GBM).Besides promoting the proliferation, migration, and invasion of glioma cells, DHX9 facilitated the infiltration of macrophages into glioma tissues and polarization to M2-like macrophages, known as tumor-associated macrophages (TAMs). DHX9 silencing decreased the expression of colony-stimulating factor 1 (CSF1), which partially restored the inhibitory effect on malignant progress of glioma and infiltration of TAMs caused by DHX9 knockdown by targeting the transcription factor 12 (TCF12). Moreover, TCF12 could directly bind to the promoter region of CSF1. Conclusion: DHX9/TCF12/CSF1 axis regulated the increases in the infiltration ofTAMs to promote glioma progression and might be a novel potential target for future immune therapies against gliomas.

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“…MiR-186 was found to be abnormally expressed in a variety of cancers. Specifically, miR-186 is down-regulated in prostate cancer 16 , hepatocellular carcinoma 17 , and breast cancer 18 , but upregulated in lung adenocarcinoma 19 and glioma 20 . However, the correlation between miR-186 and BCa has not been focused yet.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-186 suppresses cell proliferation and metastasis in bladder cancer

Liang¹,

Li²,

Zhu³

et al. 2022

Afr H. Sci.

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Purpose: Bladder cancer (BCa) is a common malignancy in the urinary system. This study aims to explore the role of miR-186 in BCa tumorigenesis. Methods: The expression of miR-186 and ADAMTS12 in clinical BCa tissues and cell lines was detected. BCa cell lines T24, 5637 and EJ were used to transfect miR-186 mimics or inhibitors. Luciferase reporter gene detection confirmed the correlation between miR-186 and ADAMTS12. MTT method and flow cytometry were used to detect cell viability and apoptosis. Cell migration and invasion ability was detected by transwell assay. The protein level of ADAMTS12, β-catenin, GSK-3β and p-GSK-3β was determined using western blot analysis. Results: MiR-186 was negatively correlated with the expression of ADAMTS12 in BCa tissues. Further research confirmed that ADAMTS12 is the direct target of miR-186. In addition, overexpression of miR-186 down-regulated the expression of ADAMTS12,inhibiting cell viability and apoptosis, while knockout of miR-186 led to the opposite result. miR-186 also inhibits the phosphorylation of GSK-3 β and β-catenin without changing the total GSK-3β level. Our study shows that miR-186 has a negative regulatory effecton the expression of ADAMTS12 in clinical specimens and in vitro. miR-186 can inhibit the proliferation and invasion of BCa cells. Conclusions: miR-186 has the potential to be used as a biomarker in the early detection of BCa. Keywords: Purpose: Bladder cancer (BCa) is a common malignancy in the urinary system. This study aims to explore the role of miR-186 in BCatumorigenesis.Methods: The expression of miR-186 and ADAMTS12 in clinical BCa tissues and cell lines was detected. BCa cell lines T24, 5637 andEJ were used to transfect miR-186 mimics or inhibitors. Luciferase reporter gene detection confirmed the correlation between miR-186 and ADAMTS12. MTT method and flow cytometry were used to detect cell viability and apoptosis. Cell migration and invasionability was detected by transwell assay. The protein level of ADAMTS12, β-catenin, GSK-3β and p-GSK-3β was determined usingwestern blot analysis.Results: MiR-186 was negatively correlated with the expression of ADAMTS12 in BCa tissues. Further research confirmed thatADAMTS12 is the direct target of miR-186. In addition, overexpression of miR-186 down-regulated the expression of ADAMTS12,inhibiting cell viability and apoptosis, while knockout of miR-186 led to the opposite result. miR-186 also inhibits the phosphorylationof GSK-3 β and β-catenin without changing the total GSK-3β level. Our study shows that miR-186 has a negative regulatory effecton the expression of ADAMTS12 in clinical specimens and in vitro. miR-186 can inhibit the proliferation and invasion of BCa cells.Conclusions: miR-186 has the potential to be used as a biomarker in the early detection of BCa.Keywords: miR-186; ADAMTS12; bladder cancer; proliferation; metastasis biomarker.

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The long noncoding RNA FTX promotes a malignant phenotype in bone marrow mesenchymal stem cells via the miR-186/c-Met axis

Cited by 13 publications

References 33 publications

Non-Coding RNAs in Glioma Microenvironment and Angiogenesis

Non-Coding RNAs in Glioma Microenvironment and Angiogenesis

RNA‐binding protein DHX9 promotes glioma growth and tumor‐associated macrophages infiltration via TCF12

MicroRNA-186 suppresses cell proliferation and metastasis in bladder cancer

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