The long noncoding RNA MIR210HG promotes tumor metastasis by acting as a ceRNA of miR-1226-3p to regulate mucin-1c expression in invasive breast cancer

Li, Xiaoyü; Zhou, Liye; Luo, Hao; Zhu, Qi; Zuo, Liang; Liu, Guyue; Chu, Feng; Zhao, Jie; Zhang, Yuanyuan; Li, Xue

doi:10.18632/aging.102149

Cited by 60 publications

(53 citation statements)

References 41 publications

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“…Other studies have revealed that MIR210HG overexpression could effectively improve the damaged invasion and migration abilities of HTR8/SVneo cells in circumstances of H/R. According to related studies, MIR210HG may play its role by targeting the regulation of miR-1226-3p [15]. We also noted that the expression of miR-1226-3p underwent a significant change in the presence of MIR210HG overexpression.…”

Section: Discussionsupporting

confidence: 61%

The long noncoding RNA MIR210HG improves preeclampsia by regulating miR-1226-3p/MUC1-C: in vitro study

2020

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Introduction: The purpose of this study was to explore the effects and mechanisms of the long noncoding RNA (lncRNA) MIR210HG in preeclampsia development. Material and methods: We collected placental tissue from 25 patients with preeclampsia (PE) and 25 healthy pregnant women and analysed the differences in lncRNAs between the placental tissues. HTR8/SVneo cells were transfected with the MIR210HG from the samples, and we observed the effects on the biological activities of HTR8/SVneo cells in conditions of hypoxia and reoxygenation (H/R) by transwell and wound-healing assays. To clarify the mechanisms of action, we used the Western blot assay to measure the expression levels of relative proteins (MUC1-C, SMAD2, Snail, E-cadherin and N-cadherin) and cellular immunofluorescence to measure Erk protein nuclear volume. We also evaluated the correlations between expression of MIR210HG and that of miR-1226-3p, miR-1226-3p and MUC1-C by luciferase reporter assay. Results: Chip hybridisation and scans revealed that MIR210HG expression was significantly more depressed in patients with PE than in healthy pregnant women (p < 0.001). With MIR210HG overexpression, HTR8/SVneo cell invasion and migration were significantly increased in an environment of H/R plus pcDNA3.1, microRNA negative control (miR-NC) or hsa-miR-1226-3p in comparison with an environment of H/R alone (p < 0.001, respectively). Levels of miR-1226-3p were significantly suppressed in lncRNA samples subjected to H/R (p < 0.001, respectively). In addition, miR-1226-3p depressed MIR210HG activity, thereby ameliorating PE. Conclusions: By regulating miR-1226-3p/MUC1-C activity, MIR210HG can help ameliorate preeclampsia.

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Section: Discussionsupporting

confidence: 61%

The long noncoding RNA MIR210HG improves preeclampsia by regulating miR-1226-3p/MUC1-C: in vitro study

2020

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“…In the 373 colon cancer patients, the 5-year survival rate of patients in the MIR210HG high expression group was lower than the 5-year survival rate of patients in the MIR210HG low expression group ( Ruan et al, 2019 ). In invasive breast cancer, MIR210HG is elevated in cancer tissues, and high MIR210HG expression is positively associated with a poor prognosis ( Li et al, 2019 ). From the GSE30219 dataset, it was found that MIR210HG was upregulated in non-small cell lung cancer, the overall survival rate of the MIR210HG high expression group was lower than that of the MIR210HG low expression group, and MIR210HG could promote tumor cell proliferation and migration ( Kang et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Six-lncRNA Immune Prognostic Signature for Cervical Cancer

Chen

Huang

et al. 2020

Front. Genet.

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Background This study searched for immune-related long noncoding RNAs (lncRNAs) to predict the prognosis of patients with cervical cancer. Method We obtained immunologically relevant lncRNA expression profiles and clinical follow-up data from cervical cancer patients from The Cancer Genome Atlas database and the Molecular Signatures Database. Cervical cancer patients were randomly divided into a training group, testing group and combined group. The immune prognostic signature was constructed by Least Absolute Shrinkage and Selection Operator Cox regression, prognosis was analyzed by Kaplan–Meier curves between different groups, and the accuracy of the prognostic model was assessed by receiver operating characteristic-area under the curve (ROC-AUC) analysis. Results A six-lncRNA immune prognostic signature (LIPS) was constructed to predict the prognosis of cervical cancer. The six lncRNAs are as follows: AC009065.8, LINC01871, MIR210HG, GEMIN7-AS1, GAS5-AS1, and DLEU1. A ROC-AUC analysis indicated that the model could predict the prognosis of cervical cancer patients in different subgroups. A Kaplan–Meier analysis showed that patients with high risk scores had a poor prognosis; these results were equally meaningful in the subgroup analyses. Risk scores differed depending on the clinical pathology and tumor grade and were independent risk factors for cervical cancer prognosis. Gene set enrichment analysis revealed an association between the LIPS and the immune response, Wnt signaling pathway, and TGF beta signaling pathway. Conclusion Our study shows that the six-LIPS can predict the prognosis of cervical cancer and contribute to decisions regarding the immunotherapeutic strategy.

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“…More and more reports have found that lncRNAs can function as ceRNAs of miRNAs, and inhibit the expression of miRNAs as molecular sponges. For example, in breast cancer, MIR210HG acts as ceRNA of miR-1226-3p to regulate the expression of mucin-1c, promoting the occurrence of metastasis; 21 in hepatocellular carcinoma, XIST regulates PDCD4 by targeting miR-497-5p to regulate the proliferation and migration of cancer cells. 22 In this study, we identified the interaction between miR-513b-5p with AZIN1-AS1.…”

Section: Discussionmentioning

confidence: 99%

<p>AZIN1-AS1, A Novel Oncogenic LncRNA, Promotes the Progression of Non-Small Cell Lung Cancer by Regulating MiR-513b-5p and DUSP11</p>

Cai

Liu

et al. 2020

OTT

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Background: Emerging researches have demonstrated that aberrantly expressed long noncoding RNAs (lncRNAs) have great significance in non-small cell lung cancer (NSCLC) progression. The aim of this study was to explore the role of lncRNA AZIN1 antisense RNA 1 (AZIN1-AS1) in NSCLC and the related mechanism. Methods: Expressions of AZIN1-AS1 and miR-513b-5p in NSCLC samples were detected by qRT-PCR. NSCLC cell lines (H1299 and HCC827) were used in vitro assays. CCK-8 assay, EdU assay, wound healing test and Transwell assay were carried out to test the biological influence of AZIN1-AS1 on NSCLC cells. Subcutaneous xenotransplanted tumor model and tail vein injection model were established to test the role of AZIN1-AS1 in vivo. Interactions between AZIN1-AS1 and miR-513b-5p, miR-513b-5p and dual-specificity phosphatase 11 (DUSP11) were determined by bioinformatic analysis, qRT-PCR, Western blot, and luciferase reporter assay. Results: AZIN1-AS1 was up-regulated in NSCLC cells and tissues, while miR-513b-5p was significantly down-regulated. Silencing of AZIN1-AS1 or overexpression of miR-513b-5p markedly inhibited proliferation, migration and invasion of NSCLC cells, while overexpression of AZIN1-AS1 or inhibition of miR-513b-5p functioned oppositely. Importantly, AZIN1-AS1 mediated the promotion of malignancy of NSCLC cells was reversed by miR-513b-5p mimics. What's more, AZIN1-AS1 could down-regulate miR-513b-5p via sponging it, and there existed a negative correlation between AZIN1-AS1 expression and miR-513b-5p expression in NSCLC samples. AZIN1-AS1 also enhanced the expression levels of DUSP11, which was proved as a target gene of miR-513b-5p. Further in vivo experiments showed that silencing of AZIN1-AS1 decreased tumor growth and metastasis, which was accompanied by overexpression of miR-513b-5p and inhibition of DUSP11 in tumor tissues. Conclusion: AZIN1-AS1 acts as a tumor promoter in NSCLC, which is ascribed to the regulation of miR-513b-5p and DUSP11.

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The long noncoding RNA MIR210HG promotes tumor metastasis by acting as a ceRNA of miR-1226-3p to regulate mucin-1c expression in invasive breast cancer

Cited by 60 publications

References 41 publications

The long noncoding RNA MIR210HG improves preeclampsia by regulating miR-1226-3p/MUC1-C: in vitro study

The long noncoding RNA MIR210HG improves preeclampsia by regulating miR-1226-3p/MUC1-C: in vitro study

Six-lncRNA Immune Prognostic Signature for Cervical Cancer

<p>AZIN1-AS1, A Novel Oncogenic LncRNA, Promotes the Progression of Non-Small Cell Lung Cancer by Regulating MiR-513b-5p and DUSP11</p>

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