The long view: Considering spindle orientation in image analysis

Balough, Julia L.; Fontaine, Alexandra S. E.; Machlin, Jordan H.; Rowley, Jennifer E.; Simon, Leah E; Duncan, Francesca E.; Hornick, Jessica E.

doi:10.1002/mrd.23313

Cited by 1 publication

(1 citation statement)

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“…Spindles and chromosome alignment were considered normal if spindles exhibited a bipolar structure with all chromosomes aligned on the metaphase plate. Only the oocytes with spindles parallelly oriented to the imaging plane were used for this analysis to accurately determine chromosome alignment (Balough et al, 2020). All images were processed and analyzed using FIJI (National Institutes of Health; Schindelin et al, 2012Schindelin et al, , 2015.…”

Section: Assessment Of Oocyte Maturation Status and Spindle Architect...mentioning

confidence: 99%

Cumulus expansion is impaired with advanced reproductive age due to loss of matrix integrity and reduced hyaluronan

Babayev,

Suebthawinkul,

Gokyer

et al. 2023

Aging Cell

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Reproductive aging is associated with ovulatory defects. Age‐related ovarian fibrosis partially contributes to this phenotype as short‐term treatment with anti‐fibrotic compounds improves ovulation in reproductively old mice. However, age‐dependent changes that are intrinsic to the follicle may also be relevant. In this study, we used a mouse model to demonstrate that reproductive aging is associated with impaired cumulus expansion which is accompanied by altered morphokinetic behavior of cumulus cells as assessed by time‐lapse microscopy. The extracellular matrix integrity of expanded cumulus–oocyte complexes is compromised with advanced age as evidenced by increased penetration of fluorescent nanoparticles in a particle exclusion assay and larger open spaces on scanning electron microscopy. Reduced hyaluronan (HA) levels, decreased expression of genes encoding HA‐associated proteins (e.g., Ptx3 and Tnfaip6), and increased expression of inflammatory genes and matrix metalloproteinases underlie this loss of matrix integrity. Importantly, HA levels are decreased with age in follicular fluid of women, indicative of conserved reproductive aging mechanisms. These findings provide novel mechanistic insights into how defects in cumulus expansion contribute to age‐related infertility and may serve as a target to extend reproductive longevity.

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Section: Assessment Of Oocyte Maturation Status and Spindle Architect...mentioning

confidence: 99%