The major surface protease (MSP or GP63) of Leishmania sp. Biosynthesis, regulation of expression, and function

Yao, Chaoqun; Donelson, John E.; Wilson, Mary E.

doi:10.1016/s0166-6851(03)00211-1

Cited by 272 publications

(254 citation statements)

References 117 publications

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“…Previous studies already showed that the gp63 from T. cruzi has a key role in parasite infection [3] and complement inhibition [55]. We identified 7 clusters of this protein, and most of the hits were found in the EVs from Y strain.…”

Section: Discussionmentioning

confidence: 87%

Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells

Ribeiro

Vasconcellos

Soares

et al. 2018

J of Extracellular Vesicle

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Trypanosoma cruzi, the aetiologic agent of Chagas disease, releases vesicles containing a wide range of surface molecules known to affect the host immunological responses and the cellular infectivity. Here, we compared the secretome of two distinct strains (Y and YuYu) of T. cruzi, which were previously shown to differentially modulate host innate and acquired immune responses. Tissue culture-derived trypomastigotes of both strains secreted extracellular vesicles (EVs), as demonstrated by electron scanning microscopy. EVs were purified by exclusion chromatography or ultracentrifugation and quantitated using nanoparticle tracking analysis. Trypomastigotes from YuYu strain released higher number of EVs than those from Y strain, enriched with virulence factors trans-sialidase (TS) and cruzipain. Proteomic analysis confirmed the increased abundance of proteins coded by the TS gene family, mucin-like glycoproteins, and some typical exosomal proteins in the YuYu strain, which also showed considerable differences between purified EVs and vesicle-free fraction as compared to the Y strain. To evaluate whether such differences were related to parasite infectivity, J774 macrophages and LLC-MK2 kidney cells were preincubated with purified EVs from both strains and then infected with Y strain trypomastigotes. EVs released by YuYu strain caused a lower infection but higher intracellular proliferation in J774 macrophages than EVs from Y strain. In contrast, YuYu strain-derived EVs caused higher infection of LLC-MK2 cells than Y strain-derived EVs. In conclusion, quantitative and qualitative differences in EVs and secreted proteins from different T. cruzi strains may correlate with infectivity/virulence during the host–parasite interaction.

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Section: Discussionmentioning

confidence: 87%

Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells

Ribeiro

Vasconcellos

Soares

et al. 2018

J of Extracellular Vesicle

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“…Importantly, we identified specific targets for the VIP derivatives on the parasite surface. The plasma membrane of the promastigote stage possesses a thick anionic glycocalix made up mostly of LPG (48) and the external GPI-anchored metalloproteinase gp63 (49). Both components are involved in the interaction with and partial resistance to antimicrobial peptides (50).…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Efficacy of Stable Analogues of Vasoactive Intestinal Peptide against Pathogens

Campos‐Salinas

Cavazzuti

O’Valle³

et al. 2014

Journal of Biological Chemistry

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Background: Antimicrobial properties of the anti-inflammatory neuropeptide VIP are limited by its unstable nature. Results: The VIP derivatives protected against polymicrobial sepsis and cutaneous leishmaniasis by selectively killing pathogens through membrane-disrupting mechanisms. Conclusion: Modification of critical residues in the native VIP sequence generates stable peptides with potent antimicrobial activities in vitro and in vivo. Significance: This work indicates a molecular rationale for designing new agents against drug-resistant infectious diseases.

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“…[154], which is distributed over the entire surface of the promastigote forms, including the flagellum [155][156][157]. Each L. major promastigote in the stationary phase is estimated to have 500,000 copies of gp63, constituting about 1% of the organism's total protein content [158].…”

Section: Metallopeptidasesmentioning

confidence: 99%

Biological Roles of Peptidases in Trypanosomatids~!2009-11-26~!2010-02-15~!2010-03-18~!

Vermelho

Branquinha²,

d’Avila-Levy³

et al. 2010

TOPARAJ

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Abstract:In this review, we report the recent developments in the characterization of peptidases and their possible biological functions in the Trypanosomatidae family. The focus will be on peptidases from Trypanosoma cruzi, Leishmania spp., African trypanosomes and plant and insect trypanosomatids. There are numerous events in parasite development where the involvement of peptidases has been established, and they will be approached in the present review. Also in this review we will discuss the central roles have been proposed for peptidases in diverse processes such as virulence, host cell interaction and invasion, catabolism of host proteins, differentiation, cell cycle progression and both stimulation and evasion of host immune responses.

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The major surface protease (MSP or GP63) of Leishmania sp. Biosynthesis, regulation of expression, and function

Cited by 272 publications

References 117 publications

Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells

Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells

Therapeutic Efficacy of Stable Analogues of Vasoactive Intestinal Peptide against Pathogens

Biological Roles of Peptidases in Trypanosomatids~!2009-11-26~!2010-02-15~!2010-03-18~!

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