The manipulation of gene expression and the biosynthesis of Vitamin C, E and folate in light-and dark-germination of sweet corn seeds

Liu, Fengyuan; Xiang, Nan; Hu, Jisheng; Xie, Lin; Brennan, Charles S.; Huang, Wenjie; Guo, Xinbo

doi:10.1038/s41598-017-07774-9

Cited by 28 publications

(29 citation statements)

References 65 publications

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“…The method used for AsA determination was modified from previous research . A sample was extracted using 0.1% oxalic acid and detected by high‐performance liquid chromatography (HPLC) at 245 nm wave length.…”

Section: Methodsmentioning

confidence: 99%

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Effects of temperature stress on the accumulation of ascorbic acid and folates in sweet corn (Zea mays L.) seedlings

Xiang

Teng

et al. 2019

J Sci Food Agric

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BACKGROUND: Extreme temperatures are among the primary abiotic stresses that affect plant growth and development. Ascorbic acid (AsA) is an efficient antioxidant for scavenging relative oxygen species accumulated under stress. Folates play a significant role in DNA synthesis and protect plants against oxidative stress. Sweet corn (Zea mays L.), a crop grown worldwide, is sensitive to extreme temperatures at seedling stage, which may cause yield loss. This study was conducted to explore the biosynthetic regulative mechanism of AsA and folates in sweet corn seedlings under temperature stress. RESULTS:The AsA and folate composition and relative gene expression in sweet corn seedlings grown under different temperature stresses (10, 25, and 40 ∘ C) were evaluated. The imposition of temperature stress altered the AsA content mainly by modulating the expression of Zm DHAR, whose encoded enzyme dehydroascorbic reductase (DHAR) is essential in the AsA recycle pathway. Low temperature stress raised the expressions of relative genes, leading to folate accumulation. High temperature stress modulated the folate content by influencing the expression of the correspondence gene for aminodeoxychorismate synthase, Zm ADCS, as well as downstream genes that connected with DNA methylation. CONCLUSION: These results provided a theoretical basis, at a genetic level, for understanding the stress responses mechanism in sweet corn seedlings, offering guidance for sweet corn cultivation.

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Section: Methodsmentioning

confidence: 99%

“…The method reported previously was modified and used for the extraction of folate. The contents of folate derivatives were detected by HPLC with fluorimetric detection (HPLC‐FL) system, which was equipped with a Waters 2475 Multi λ fluorescence detector (Waters Corporation, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Effects of temperature stress on the accumulation of ascorbic acid and folates in sweet corn (Zea mays L.) seedlings

Xiang

Teng

et al. 2019

J Sci Food Agric

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“…Conversely, the DHAR contributes in ascorbate-glutathione cycle (AsA-GSH), catalysed by dehydroacorbate reductase enzyme. This step improves ascorbic acid level in plants and contributes to scavenging free radicals (Liu et al, 2017). The effect of PGRs treatments on the relative expression levels of three key-encoding genes ( Gm VTC2, Gm GLDH and Gm DHAR) involve in vitamin C biosynthesis was analysed by Real-time PCR.…”

Section: Effect Of Pgrs On Gene Expression Level Involve In Vitamin Cmentioning

confidence: 99%

Influence of plant growth regulators on key‐coding genes expression associated with phytochemicals biosynthesis and antioxidant activity in soybean (Glycine max (L.) Merr) sprouts

Guo

Wang

Chang

et al. 2018

Int J of Food Sci Tech

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Summary The present study was aimed to investigate the effect of plant growth regulators (PGRs) on phytochemicals biosynthesis and antioxidant activity in soybean sprouts. GmVTC2, GmDHAR and GmGLDH were investigated for the biosynthesis of vitamin C. GmPAL, GmCHS and GmIFS were investigated for the biosynthesis of phenolics, flavonoids and isoflavones. Our results revealed that 6‐benzylaminopurine (6‐BA) treatment significantly affected the expression levels of those major genes, followed by Gibberellin (GA3) and Naphthaleneacetic acid (NAA). Moreover, the highest levels of vitamin C, polyphenolics and antioxidant activity in 6‐BA and GA3 treated groups indicate the synergistic and antagonistic role of growth regulators in the biosynthesis of phytochemicals. Overall, 6‐BA seems the key contributor in genes’ expression involve in the biosynthesis secondary metabolites and their antioxidant activity. Therefore, it could be the most suitable option to enhance nutritional quantity and health benefits of soybean sprouts.

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“…Reactions in the free radical chain oxidation mechanism and antioxidative reaction of vitamin E. The figure was modified from Refs. and . α‐Toc•, α‐tocopheroxyl radical; α‐TocH, α‐tocopherol; GSH, Glutathione; H 2 O 2 , hydrogen peroxide; HOO•, hydroperoxyl radical; L•, lipid radical; low density lipoprotein; LH, unsaturated fatty acid; In, initiator; LO•, alkoxy radical; LOO•, lipid peroxyl radical; LOOH, lipid hydroperoxide; O 2 − •, superoxide anion; HO•, hydroxyl radical; ROO•, peroxyl radical.…”

Section: Introductionmentioning

confidence: 99%

Vitamin E: Regulatory Redox Interactions

et al. 2019

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Vitamin E is an essential nutrient that was discovered in the 1920s. Many of the physiological functions of vitamin E, including its antioxidative effects, have been studied for nearly 100 years. Changes in redox balance induced by both endogenously and exogenously generated reactive oxygen species (ROS) are involved in various diseases, and are also a phenomenon that is considered essential for survival. Vitamin E is known to regulate redox balance in the body due to its high concentration among the lipid soluble vitamin groups, and exists ubiquitously in the whole body, including cell membranes and lipoproteins. However, it has been reported that the beneficial properties of vitamin E, including its antioxidative effects, are only displayed in vitro, and not in vivo. Therefore, there exists an ongoing debate regarding the biological functions of vitamin E and its relationship with redox balance. In this review, we introduce the relationship between vitamin E and redox interactions with (i) absorption, distribution, metabolism, and excretion of vitamin E, (ii) oxidative stress and ROS in the body, (iii) mechanism of antioxidative effects, (iv) non‐antioxidant functions of vitamin E, and (v) recent recognition of the field of oxidative stress research. Understanding the recent findings of the redox interaction of vitamin E may help to elucidate the different antioxidative phenomena observed for vitamin E in vitro and in vivo. © 2019 IUBMB Life, 71(4):430–441, 2019

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The manipulation of gene expression and the biosynthesis of Vitamin C, E and folate in light-and dark-germination of sweet corn seeds

Cited by 28 publications

References 65 publications

Effects of temperature stress on the accumulation of ascorbic acid and folates in sweet corn (Zea mays L.) seedlings

Effects of temperature stress on the accumulation of ascorbic acid and folates in sweet corn (Zea mays L.) seedlings

Influence of plant growth regulators on key‐coding genes expression associated with phytochemicals biosynthesis and antioxidant activity in soybean (Glycine max (L.) Merr) sprouts

Vitamin E: Regulatory Redox Interactions

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