The membrane complement regulatory protein CD59 and its association with rheumatoid arthritis and systemic lupus erythematosus

Das, Nibhriti; Anand, Devyani; Biswas, Bintili; Kumari, Deepa; Gandhi, Monika

doi:10.1016/j.cmrp.2019.07.013

Cited by 4 publications

(1 citation statement)

References 55 publications

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“…However, NP or TNFα stimulation did not induce any significant changes in the studied cells. In a rat model with collagen-induced arthritis, it was found that inhibition of CD59 further enhanced the inflammatory response, whereas intra-articular injection of CD59 reversed this effect [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

Complement Regulation in Immortalized Fibroblast-like Synoviocytes and Primary Human Endothelial Cells in Response to SARS-CoV-2 Nucleocapsid Protein and Pro-Inflammatory Cytokine TNFα

Franke

Schulze‐Tanzil

Braun

et al. 2022

Life

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Background: Case reports are available showing that patients develop symptoms of acute arthritis during or after recovery from SARS-CoV-2 infection. Since the interrelation is still unknown, our aim was to study the impact of the SARS-CoV-2 nucleocapsid protein (NP) on human fibroblast-like synoviocytes and human endothelial cells (hEC) in terms of complement and cytokine regulation. Methods: Non-arthritic (K4IM) synoviocyte, arthritic (HSE) synoviocyte cell lines and primary hEC were stimulated with recombinant NP and/or TNFα. Analyses of cell viability, proliferation, gene and protein expression of cytokines and complement factors were performed. Results: NP suppressed significantly the vitality of hEC and proliferation of HSE. NP alone did not induce any significant changes in the examined gene expressions. However, NP combined with TNFα induced significantly higher TNFα in HSE and K4IM as well as higher IL-6 and CD55 gene expression in HSE and suppressed C3aR1 gene expression in hEC. HSE proliferated twice as fast as K4IM, but showed significantly lesser gene expressions of CD46, CD55, CD59 and TNFα with significantly higher IL-6 gene expression. CD35 gene expression was undetectable in K4IM, HSE and hEC. Conclusions: NP might contribute in combination with other inflammatory factors to complement regulation in arthritis.

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Section: Discussionmentioning

confidence: 99%