The microfilament system and malignancy

Lindberg, Uno; Karlsson, Roger; Lassing, Ingrid; Schutt, Clarence E.; Höglund, Anders

doi:10.1016/j.semcancer.2007.10.002

Cited by 67 publications

(64 citation statements)

References 127 publications

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“…Actin is an essential component of the microfilament system, whereas zyxin is a major focal adhesion protein often used as a marker of these structures (27,28). As shown in Fig.…”

Section: Although Gmentioning

confidence: 99%

Two-color Photoactivatable Probe for Selective Tracking of Proteins and Cells

Welman

Serrels

Brunton

et al. 2010

Journal of Biological Chemistry

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We report the development and application of photoactivatable Green Cherry (G PA C), the first genetically encoded "continuously red-photoactivatable green" two-color probe for live cell imaging. G PA C is unique in that it enables real-time tracking of selected subpopulations of proteins and organelles in the cell or of cells within tissues and whole organisms, with constant reference to the entire population of the probe. Using G PA C-zyxin as proof of utility, we obtained new insights into the dynamic movement of the cytoskeletal protein zyxin. We show that zyxin is continuously and rapidly recruited from the cytosol into established focal adhesions. It can also move rapidly within a given focal adhesion and "hop" between adjacent focal adhesions, emphasizing the dynamic nature of proteins within these structures. The in vivo utility of G PA C is exemplified by tracking hemocyte movements using a versatile transgenic Drosophila model engineered to express G PA C in tissues and cells of interest under the control of the GAL4-inducible promoter.

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“…Actin is an essential component of the microfilament system, whereas zyxin is a major focal adhesion protein often used as a marker of these structures (27,28). As shown in Fig.…”

Section: Although Gmentioning

confidence: 99%

Two-color Photoactivatable Probe for Selective Tracking of Proteins and Cells

Welman

Serrels

Brunton

et al. 2010

Journal of Biological Chemistry

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“…11 Transformation into a malignant state is strongly associated with modulation of actin cytoskeletal genes. 12 In this regard, Schenker et al and Gonos et al identified SM22 as a transformation-sensitive gene that is down-regulated after transformation with the ras oncogene or SV40. 13,14 SM22 expressed in nontransformed rat intestinal epithelial RIE-1 cells and its expression was down-regulated after oncogenic transformation with K-Ras.…”

mentioning

confidence: 99%

“…12 The purpose of this study was to investigate whether SM22 down-regulation also occurred in human colorectal cancers and to address the pathological functions in colorectal carcinogenesis. We performed Western blot and immunohistochemical staining in 81 colorectal specimens to evaluate SM22 expression.…”

mentioning

confidence: 99%

Loss of SM22 is a characteristic signature of colon carcinogenesis and its restoration suppresses colon tumorigenicity in vivo and in vitro

Yeo

Park

Kim

et al. 2010

Cancer

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BACKGROUND:We previously found the down-expression of SM22 in an experimental animal model of colorectal cancer by performing a proteomic analysis. In this study, we addressed the expression and molecular mechanisms of SM22 in human colorectal cancer. METHODS: To evaluate the expression of SM22 in colon cancers, Western blot and immunohistochemistry were performed in 13 normal, 14 adenomas, and 44 adenocarcinomas. To address the role of SM22 in colon carcinogenesis, SM22 was restored in the colon cancer cells by the transfection with the pIRES2 vector containing full-length SM22 cDNA and tested for tumorigenicity in vivo and in vitro. RESULTS: SM22 was found to be significantly down-regulated in adenocarcinoma (58%) compared with adenoma (21.4%) and normal (15.3%). The loss of SM22 correlated with poor differentiation of tumor (P ¼ 0.009) and lymph node metastasis (P ¼ 0.029). Restoration of SM22 expression inhibited cell migration, colony-forming ability of cancer cells, and induced retardation of in vivo tumor growth in a xenograft model. CONCLUSIONS: Loss of SM22 is a molecular signature of colon cancer and is closely associated with progression, differentiation, and metastasis of colon cancer. The restoration of SM22 leads to an inhibition of colon carcinogenesis in vivo and in vitro, suggesting that SM22 might potentially function as a novel tumor suppressor. Cancer 2010;116:2581-9.

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“…It also participates in plant cell responses to internal and external signals (Fatehi et al 2012). Profilin is involved in signaling pathways, which promotes rapid formation of actin filaments (Ridley et al 2003;Lindberg et al 2008;Yu 2008). Actin polymerization also involves formins which belong to a different family of multimodular proteins .…”

Section: Structure and Organization Of The Cytoskeletonmentioning

confidence: 99%

ABA pretreatment can limit salinity-induced proteome changes in growing barley sprouts

2017

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A proteomic study was conducted on caryopses of barley cv. Stratus (Hordeum vulgare). The caryopses were germinated in darkness at 20°C, according to one of the three experimental setups: (a) in distilled water for 24 h, followed by 100 mM NaCl for another 24 h (salinity stress, SS); (b) in 100 lM abscisic acid for 24 h, with rinsing in distilled water to remove residual ABA, followed by 100 mM NaCl for another 24 h (pretreatment with ABA ? salinity stress, ABAS); and (c) only in distilled water, for comparison with the tested samples (control, C). After 48 h, proteomes were extracted from barley sprouts in each treatment, according to the method by Gallardo et al. (2002a, b, modified). Proteins were then separated by 2DE and analyzed by MALDI-TOF/MS (Bruker). Under salinity stress, we observed changes in the pattern of proteins involved in: (a) energy metabolism, (b) one-carbon metabolism, (c) cell wall biogenesis, lignification, and adhesion, (d) response to oxidative stress, desiccation stress, and other defense responses, (e) structure and organization of the cytoskeleton, (f) signal transduction, transcription and translation processing, and (g) protein turnover. Our results revealed that ABA can alter the response of barley seedlings to salinity stress. In general, ABA pretreatment limited stress-induced proteomic changes. Alterations in energy and one-carbon metabolism indicate the importance of ABA in glycolytic/gluconeogenic pathways and the SMM cycle during salinity stress. Other observations demonstrated the role of ABA in hemicellulose, cellulose and lignin biosynthesis, ROS scavenging, actin CK formation, control of PI signaling, chromatin-mediated mechanism of stress tolerance, mRNA stability, and protection against dehydration.

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The microfilament system and malignancy

Cited by 67 publications

References 127 publications

Two-color Photoactivatable Probe for Selective Tracking of Proteins and Cells

Two-color Photoactivatable Probe for Selective Tracking of Proteins and Cells

Loss of SM22 is a characteristic signature of colon carcinogenesis and its restoration suppresses colon tumorigenicity in vivo and in vitro

ABA pretreatment can limit salinity-induced proteome changes in growing barley sprouts

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