The Microfluidic Microwell Device Integrating Surface Enhanced Raman Scattering for Bacteria Enrichment and in Situ Antibiotic Susceptibility Test

Liao, Cheng-Chieh; Huang, Hsiu-Kang; Chen, Yi‐Zih; Huang, Nien-Tsu

doi:10.1109/mems46641.2020.9056435

Cited by 1 publication

(1 citation statement)

References 15 publications

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“…Chang et al presented the development of an integrated multimodal microfluidic system capable of performing on-chip enrichment of bacteria, collection of metabolites, and in situ SERS measurements for AST, with a limit of detection (LoD) of 10 3 CFU/mL [ 162 ]. Liao et al reported the development of a microfluidic platform integrating SERS with microwells allowing for low concentration (10 3 CFU/mL) encapsulation of bacteria followed by label-free detection and in situ AST [ 163 ].…”

Section: Microfluidics and Lab-on-a-chip Technologies Towards Rapimentioning

confidence: 99%

Rapid Methods for Antimicrobial Resistance Diagnostics

et al. 2021

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Antimicrobial resistance (AMR) is one of the most challenging threats in public health; thus, there is a growing demand for methods and technologies that enable rapid antimicrobial susceptibility testing (AST). The conventional methods and technologies addressing AMR diagnostics and AST employed in clinical microbiology are tedious, with high turnaround times (TAT), and are usually expensive. As a result, empirical antimicrobial therapies are prescribed leading to AMR spread, which in turn causes higher mortality rates and increased healthcare costs. This review describes the developments in current cutting-edge methods and technologies, organized by key enabling research domains, towards fighting the looming AMR menace by employing recent advances in AMR diagnostic tools. First, we summarize the conventional methods addressing AMR detection, surveillance, and AST. Thereafter, we examine more recent non-conventional methods and the advancements in each field, including whole genome sequencing (WGS), matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) spectrometry, Fourier transform infrared (FTIR) spectroscopy, and microfluidics technology. Following, we provide examples of commercially available diagnostic platforms for AST. Finally, perspectives on the implementation of emerging concepts towards developing paradigm-changing technologies and methodologies for AMR diagnostics are discussed.

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Section: Microfluidics and Lab-on-a-chip Technologies Towards Rapimentioning

confidence: 99%