The miR396-GRFs Module Mediates the Prevention of Photo-oxidative Damage by Brassinosteroids during Seedling De-Etiolation in Arabidopsis

Wang, Lingyan; Tian, Yanchen; Su, Wen Pang; Yu, Ping; Hu, Yifei; Lv, Jinyin; Fu, Chunxiang; Fan, Min; Bai, Ming-Yi

doi:10.1105/tpc.20.00057

Cited by 37 publications

(23 citation statements)

References 55 publications

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“…This theory provides strong experimental support for the starch-sugar hypothesis (Li et al 2020a). Wang et al (2020a) found BRs play an important role in the process of etiolated seedlings in Arabidopsis thaliana, BRs mutant det2-1 (de-etiolated2) relative to the wild type, containing excessive protochlorophyllide, the gain-of function mutant bzr1-1D (brassinazole-resistant 1-1D) suppressed the protochlorophyllide accumulation of det2-1, thereby promoting greening of etiolated seedlings. However, BRs-deletion and BRs-insensitive mutants grew in darkness for 4-8 days, and after exposure to light for 2 days, the green turning ability of seedlings was significantly lower than that of wild-type.…”

Section: Role Of Brs In Plant Growth and Developmentmentioning

confidence: 63%

“…However, BRs-deletion and BRs-insensitive mutants grew in darkness for 4-8 days, and after exposure to light for 2 days, the green turning ability of seedlings was significantly lower than that of wild-type. Further analysis showed that BZR1, the Phytochrome-Interacting Factors 4 (PIF4) and GROWTH REGULATING FACTOR 7 (GRF7) coordinated the expression of genes encoding key enzymes in chlorophyll biosynthesis, and thus promoted the etiolated seedlings to become green (Wang et al 2020a).…”

Section: Role Of Brs In Plant Growth and Developmentmentioning

confidence: 99%

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Roles of brassinosteroids in plant growth and abiotic stress response

Zheng

Lin

et al. 2020

Plant Growth Regul

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Brassinosteroids (BRs) are widely used class of natural steroidal plant hormones. BRs take part in the regulation of growth and development of plants, maintaining BRs homeostasis, and allowing adaptation to environmental changes through the life cycle. They also play an important role in abiotic stress responses such as drought, salinity, high temperature, low temperature and heavy metal stresses. Through the signal transduction pathway, BRs interact with a variety of transcription factors via a series of phosphorylation cascades to regulate the expression of BR target genes. Thus, they regulate the various growth and development processes of plants. BRs crosstalk with different hormones to regulate plant physiology and development. This review primarily introduces the signaling pathways of BRs, their role in regulating plant growth and development, abiotic stresses, and their interaction with other plant hormones at the transcriptional and post-transcriptional levels. Our review of this topic will provide a complete reference for the study and utilization of BRs in the future.

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Section: Role Of Brs In Plant Growth and Developmentmentioning

confidence: 63%

Section: Role Of Brs In Plant Growth and Developmentmentioning

confidence: 99%

Roles of brassinosteroids in plant growth and abiotic stress response

Zheng

Lin

et al. 2020

Plant Growth Regul

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“…Researches show that most GRFs are target genes of microRNA396 (miR396), which participate in the growth and development of various plants along with miR396 [6,8,26,27]. As the…”

Section: Prediction Of Potential Targets For Small Rnamentioning

confidence: 99%

Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas

et al. 2021

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GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species’ GRF genes and their expression patterns is still lacking. In this study, we characterized the 10 JcGRF genes. A detailed investigation into the physic nut GRF gene family is performed, including analysis of the exon-intron structure, conserved domains, conserved motifs, phylogeny, chromosomal locations, potential small RNA targets and expression profiles under both normal growth and abiotic stress conditions. Phylogenetic analysis indicated that the 10 JcGRF genes were classified into five groups corresponding to group I, II, III, IV and V. The analysis of conserved domains showed that the motifs of JcGRF genes were highly conserved in Jatropha curcas. Expression analysis based on RNA-seq and qRT-PCR showed that almost all JcGRF genes had the highest expression in seeds, but very low expression was detected in the non-seed tissues tested, and four JcGRF genes responded to at least one abiotic stress at at least one treatment point. Our research will provide an important scientific basis for further research on the potential functions of JcGRF genes in Jatropha curcas growth and development, and response to abiotic stress, and will eventually provide candidate genes for the breeding of Jatropha curcas.

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“…More specifically, the level of chlorophyll a and b was reduced by 16.0 and 37.5% in the MIR396/NaCl sample compared to the MIR396/Ns sample (Figure 2F,G). Interestingly, it was previously reported that via its posttranscriptional regulation of the abundance of the GRF7 and GRF8 transcripts, miR396 may play an indirect role in regulating the biosynthesis of chlorophyll [64]. In direct contrast to the mild differences in the level of the two primary photosynthetic pigments of Col-0, MIM396, and MIR396 plants to the applied stress, the content of anthocyanin in the MIR396/NaCl sample was dramatically elevated by 135.9% in the MIR396/NaCl sample (Figure 2E).…”

Section: Discussionmentioning

confidence: 99%

Molecular Manipulation of the MiR396/GRF Expression Module Alters the Salt Stress Response of Arabidopsis thaliana

et al. 2021

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We previously demonstrated that microRNA396 (miR396) abundance is altered in 15-day-old Arabidopsis thaliana (Arabidopsis) whole seedlings following their exposure to a 7-day salt stress treatment regime. We, therefore, used a molecular modification approach to generate two new Arabidopsis transformant populations with reduced (MIM396 plants) and elevated (MIR396 plants) miR396 abundance. The exposure of 8-day-old wild-type Arabidopsis whole seedlings and a representative plant line of the MIM396 and MIR396 transformant populations to a 7-day salt stress treatment regime revealed unique phenotypic and physiological responses to the imposed stress by unmodified wild-type Arabidopsis plants and the MIM396 and MIR396 transformat lines. A quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) approach was, therefore, applied to demonstrate that the plant line specific responses to salt stress likely stemmed from the unique molecular profile of each of the GROWTH REGULATING FACTOR (GRF) transcription factor gene family members which form posttranscriptional targets of miR396-directed expression regulation. RT-qPCR additionally revealed that, in 15-day-old Arabidopsis whole seedlings, the three previously identified putative target genes of miR396 belonging to the NEUTRAL/ALKALINE NONLYSOSOMAL CERAMIDASE-LIKE (NCER) gene family, including NCER1, NCER2, and NCER3, do not form targets of miR396-directed expression regulation at the posttranscriptional level. Taken together, the phenotypic and molecular analyses presented here demonstrate that alteration of the miR396/GRF expression module is central to the molecular response of Arabidopsis to salt stress.

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The miR396-GRFs Module Mediates the Prevention of Photo-oxidative Damage by Brassinosteroids during Seedling De-Etiolation in Arabidopsis

Cited by 37 publications

References 55 publications

Roles of brassinosteroids in plant growth and abiotic stress response

Roles of brassinosteroids in plant growth and abiotic stress response

Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas

Molecular Manipulation of the MiR396/GRF Expression Module Alters the Salt Stress Response of Arabidopsis thaliana

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