The mitochondrial DNA copy number, cytochrome c oxidase activity and reactive oxygen species level in metaphase II oocytes obtained from in vitro culture of cryopreserved ovarian tissue in comparison with in vivo‐obtained oocyte

Amoushahi, Mahboobeh; Salehnia, Mojdeh; Ghorbanmehr, Nassim

doi:10.1111/jog.13747

Cited by 12 publications

(10 citation statements)

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“…In contrast to our results, Amoushahi, Salehnia, and Ghorbanmehr (2018) showed that oocytes matured in vitro displayed lower mtDNA copy number of those matured in vivo. These controversies may be due to the difference in species and techniques for determining the mtDNA copy number in oocytes.…”

Section: Ivm Oocytes Exhibit Increased Mtdna Copy Number Probably Bcontrasting

confidence: 99%

Mechanistic insights into the reduced developmental capacity of in vitro matured oocytes and importance of cumulus cells in oocyte quality determination

Wang

et al. 2020

Journal Cellular Physiology

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In vitro maturation of oocytes is a promising assisted reproductive technology (ART) for infertility treatment, although it is still not a routine technique for human ART due to reduced embryonic development. The aim of the present study was to clarify the possible reasons for reduced capacity of in vitro matured oocytes. Our results showed that the oocytes matured in vitro displayed increased abnormal mitochondrial distribution, reduced mitochondrial membrane potential, and increased reactive oxygen species levels when compared to in vivo matured oocytes. These results were not different in oocytes matured in vitro with or without cumulus cells. Notably, in vitro matured oocytes displayed increased mitochondrial DNA numbers probably due to functional compensation. In vitro matured oocytes showed significantly lower activation and embryonic development rates, and their ability to produce Ca2+ oscillations was much lower in response to parthenogenetic activation, especially in oocytes matured in vitro without cumulus cells with nearly half of them failing to produce calcium waves upon strontium chloride stimulation. These data are important for understanding the reasons for reduced developmental potential of in vitro matured oocytes and the importance of cumulus cells for oocyte quality.

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Section: Ivm Oocytes Exhibit Increased Mtdna Copy Number Probably Bcontrasting

confidence: 99%

Mechanistic insights into the reduced developmental capacity of in vitro matured oocytes and importance of cumulus cells in oocyte quality determination

Wang

et al. 2020

Journal Cellular Physiology

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“…The results of the present study are in line with previous studies that showed SS can be considered as an effective factor in the oocyte maturation and the rates of developmental parameters of in vitro cultured oocytes treated with SS increased significantly compared to the oocytes cultured in the medium without SS 13 , 25 . The similar effects of SS supplement in the culture medium on the development of follicle and oocyte in some mammal species have also been previously reported 23 , 26 , 28 – 31 . The obtained results may be related to the antioxidant properties of SS by reducing the ROS 13 , 26 , 28 – 31 .…”

Section: Discussionsupporting

confidence: 84%

“…It has also been shown that Sodium Selenite (SS) as a non-enzymatic antioxidant improves development of follicles, oocyte and embryo by elevating the total antioxidant capacity and reducing the level of ROS 13 , 23 – 26 . In our previous studies, it was shown that adding SS to the oocyte maturation media increased mtDNA content of the oocytes 13 , 26 . This study was designed to bring some additional information regarding the effect of SS on the biogenesis of oocyte mitochondria through TFAM expression.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Sodium Selenite on Expression of Mitochondrial Transcription Factor A during In Vitro Maturation of Mouse Oocyte

Moshaashaee¹,

Zavareh²,

Pourbeiranvand³

et al. 2021

AJMB

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Background: The aim of the present study was to investigate the effect of Sodium Selenite (SS) supplemented media on oocyte maturation, expression of mitochondrial transcription factor A (TFAM) and embryo quality. Methods: Mouse Germinal Vesicle (GV) oocytes were collected after administration of Pregnant Mare Serum Gonadotropin (PMSG); in experimental group 1, oocytes were cultured and then subjected for in vitro maturation in the absence of SS, and in experimental group 2, they were matured in vitro in the presence of 10 ng/ml of SS up to 16 hr. The control group included MII oocytes obtained from the fallopian tubes after ovarian stimulation with PMSG, followed by human chorionic gonadotropin. Then, the expression of TFAM in MII oocytes in all three groups was investigated using real-time RT-PCR. The fertilization and embryo developmental rates were assessed, and finally the quality of the blastocysts was evaluated using propidium iodide staining. Results: The oocyte maturation rate to MII stage in SS treated group was significantly higher than non-treated oocytes (75.65 vs. 68.17%, p<0.05). Also, the rates of fertilization, embryo development to blastocyst stage as well as the cell number of blastocyst in SS supplemented group were higher than other experimental group (p<0.05). There was a significant decrease in TFAM gene expression in both in vitro groups compared to the group with in vivo obtained oocytes (p<0.05). Moreover, there was a significant increase in TFAM gene expression in oocytes that matured in the presence of SS compared to that of the group without SS (p<0.05). Conclusion: Supplementation of oocyte maturation culture media with SS improved the development rate of oocytes and embryo and also enhanced TFAM expression in MII oocytes which can affect the mitochondrial biogenesis of oocytes.

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“…Although the high efficiency of energy production in mitochondria via oxidative phosphorylation pathway, it is considered as a main source of ROS. High levels of ROS lead to cell apoptosis through impairment of the mitochondrial activity in oocytes and embryos [38]. Internally produced or externally added antioxidants play important roles to maintain the oocyte’s oxidant/antioxidant balance during IVM.…”

Section: Discussionmentioning

confidence: 99%

Retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes inin vitromatured buffalo (Bubalus bubalis) oocytes

Gad

Hamed

Khalifa

et al. 2018

International Journal of Veterinary Science and Medicine

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Retinoic acid, vitamin A metabolite, plays a role in oocyte development and maturation in different ways including gene expression alteration and/or prohibiting oxidative stress. The objective of this study was to examine the effect of 9-cis-retinoic acid (9-cisRA) on the quality and maturation rate of buffalo oocytes. Cumulus-oocyte complexes (COCs, n = 460) were collected from ovaries of slaughtered buffalos. Varying concentrations of 9-cisRA (0, 5, 50, and 200 nM) were added to the maturation medium, and the following parameters were analyzed: (i) maturation and cleavage rates, (ii) mitochondrial activity and reactive oxygen species (ROS) levels, (iii) expression level of antioxidant-related genes (PRDX1, SOD1, CAT, HOMX1, and GPX4) using RT-qPCR. Maturation rate was significantly improved in 5 nM 9-cisRA oocyte group (95.8%, P < .05) compared to control and other treatment groups (86.7% in control group). The same oocyte group exhibited significantly higher mitochondrial membrane potential activity and lower ROS accumulation level compared to other treatment groups. Antioxidant-related genes were up-regulated in oocytes matured with 5 or 50 nM 9-cisRA compared to control and 200 nM 9-cisRA groups. In contrast, 200 nM of 9-cisRA showed a clear down-regulation for antioxidant-related genes except for PRDX1. In conclusion, supplementation of 9-cisRA with a lower concentration (5 nM) to the buffalo oocytes maturation media promotes maturation rate through a protection mechanism that maintains adequate levels of antioxidant-related transcripts and improves mitochondrial activity. However, 9-cisRA has no significant effect on the cleavage rate of the treated oocytes.

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The mitochondrial DNA copy number, cytochrome c oxidase activity and reactive oxygen species level in metaphase II oocytes obtained from in vitro culture of cryopreserved ovarian tissue in comparison with in vivo‐obtained oocyte

Cited by 12 publications

References 50 publications

Mechanistic insights into the reduced developmental capacity of in vitro matured oocytes and importance of cumulus cells in oocyte quality determination

Mechanistic insights into the reduced developmental capacity of in vitro matured oocytes and importance of cumulus cells in oocyte quality determination

The Effect of Sodium Selenite on Expression of Mitochondrial Transcription Factor A during In Vitro Maturation of Mouse Oocyte

Retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes inin vitromatured buffalo (Bubalus bubalis) oocytes

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