“…3 , G – I ). These data demonstrate that the mitochondrial NADH pool modulates sulfide-dependent OCR and are consistent with enhanced H 2 S clearance by cells expressing mitochondrial versus cytoplasmic Lb NOX ( 27 ). Figure 3 Dissipation of the mitochondrial NADH pool increases sulfide tolerance.…”
Section: Resultssupporting
confidence: 68%
“…In this study, we have elucidated the carbon source used for lipid synthesis by cultured cells treated with H 2 S and demonstrate that it is linked to the inhibitory effect of H 2 S on the ETC. The consequent pleiotropic effects on cell metabolism appear to prioritize glycolysis for ATP synthesis as described previously ( 27 ) and shifts operation of the tricarboxylic acid cycle (TCA) cycle in the reductive direction for redox recycling and macromolecular synthesis ( 19 ).…”
Section: Discussionmentioning
confidence: 92%
“…1 ). We have previously demonstrated that, under cell culture conditions, H 2 S is lost from the growth medium in ∼30 min ( 27 ). To observe sufficient radiolabel incorporation into the lipid pool, exogenous sulfide (100 μM) was added every 3 h over a period of 12 h and samples were collected at t = 13 h ( Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Next, we examined the effect of the sulfide oxidation enzymes SQOR and ETHE1 on the ability of sulfide to stimulate glutamine-dependent lipid synthesis. For this, we used HT29 cells in which SQOR or ETHE1 was knocked down using shRNA as previously described ( 19 , 27 ). In comparison with HT29 scr control, the sulfide effect was significantly higher in SQOR knockdown cells ( Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Studies in our laboratory have demonstrated that H 2 S affects the metabolism of both glucose and glutamine in a manner that predicts opposite effects of these carbon sources on lipid synthesis. Thus, H 2 S stimulates aerobic glycolysis and leads to the stoichiometric conversion of glucose to two equivalents of lactate ( 27 ). On the other hand, by inducing a reductive shift in the NAD + /NADH ratio, H 2 S stimulates reductive carboxylation, i.e.…”
This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
“…3 , G – I ). These data demonstrate that the mitochondrial NADH pool modulates sulfide-dependent OCR and are consistent with enhanced H 2 S clearance by cells expressing mitochondrial versus cytoplasmic Lb NOX ( 27 ). Figure 3 Dissipation of the mitochondrial NADH pool increases sulfide tolerance.…”
Section: Resultssupporting
confidence: 68%
“…In this study, we have elucidated the carbon source used for lipid synthesis by cultured cells treated with H 2 S and demonstrate that it is linked to the inhibitory effect of H 2 S on the ETC. The consequent pleiotropic effects on cell metabolism appear to prioritize glycolysis for ATP synthesis as described previously ( 27 ) and shifts operation of the tricarboxylic acid cycle (TCA) cycle in the reductive direction for redox recycling and macromolecular synthesis ( 19 ).…”
Section: Discussionmentioning
confidence: 92%
“…1 ). We have previously demonstrated that, under cell culture conditions, H 2 S is lost from the growth medium in ∼30 min ( 27 ). To observe sufficient radiolabel incorporation into the lipid pool, exogenous sulfide (100 μM) was added every 3 h over a period of 12 h and samples were collected at t = 13 h ( Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Next, we examined the effect of the sulfide oxidation enzymes SQOR and ETHE1 on the ability of sulfide to stimulate glutamine-dependent lipid synthesis. For this, we used HT29 cells in which SQOR or ETHE1 was knocked down using shRNA as previously described ( 19 , 27 ). In comparison with HT29 scr control, the sulfide effect was significantly higher in SQOR knockdown cells ( Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Studies in our laboratory have demonstrated that H 2 S affects the metabolism of both glucose and glutamine in a manner that predicts opposite effects of these carbon sources on lipid synthesis. Thus, H 2 S stimulates aerobic glycolysis and leads to the stoichiometric conversion of glucose to two equivalents of lactate ( 27 ). On the other hand, by inducing a reductive shift in the NAD + /NADH ratio, H 2 S stimulates reductive carboxylation, i.e.…”
This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
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