The mitochondrial respiratory chain

Wikström, Mårten; Pecorilla, Cristina; Sharma, Vivek

doi:10.1016/bs.enz.2023.05.001

Cited by 8 publications

(4 citation statements)

References 88 publications

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“…There are two different groups of these enzymes found in bacteria: heme-copper oxidases, including aa 3 -type cytochrome c oxidase and bo 3 -type quinol oxidase, and copper-lacking bd-type quinol oxidases, also called cytochromes bd . Heme-copper oxidases are true proton pumps, whereas cytochromes bd generate proton-motive force solely due to the vector transfer of protons along the intraprotein proton-conducting pathway, without a mechanism of proton pumping [47,[52][53][54]. Escherichia coli is a ubiquitous member of the gut microbiome of humans and warm-blooded animals.…”

Section: Introductionmentioning

confidence: 99%

Membrane-Bound Redox Enzyme Cytochrome bd-I Promotes Carbon Monoxide-Resistant Escherichia coli Growth and Respiration

Nastasi,

Borisov,

Forte

2024

IJMS

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The terminal oxidases of bacterial aerobic respiratory chains are redox-active electrogenic enzymes that catalyze the four-electron reduction of O2 to 2H2O taking out electrons from quinol or cytochrome c. Living bacteria often deal with carbon monoxide (CO) which can act as both a signaling molecule and a poison. Bacterial terminal oxidases contain hemes; therefore, they are potential targets for CO. However, our knowledge of this issue is limited and contradictory. Here, we investigated the effect of CO on the cell growth and aerobic respiration of three different Escherichia coli mutants, each expressing only one terminal quinol oxidase: cytochrome bd-I, cytochrome bd-II, or cytochrome bo3. We found that following the addition of CO to bd-I-only cells, a minimal effect on growth was observed, whereas the growth of both bd-II-only and bo3-only strains was severely impaired. Consistently, the degree of resistance of aerobic respiration of bd-I-only cells to CO is high, as opposed to high CO sensitivity displayed by bd-II-only and bo3-only cells consuming O2. Such a difference between the oxidases in sensitivity to CO was also observed with isolated membranes of the mutants. Accordingly, O2 consumption of wild-type cells showed relatively low CO sensitivity under conditions favoring the expression of a bd-type oxidase.

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Section: Introductionmentioning

confidence: 99%

Membrane-Bound Redox Enzyme Cytochrome bd-I Promotes Carbon Monoxide-Resistant Escherichia coli Growth and Respiration

Nastasi,

Borisov,

Forte

2024

IJMS

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“…As a follow up of the Research Topic–“Computational and Experimental Insights in Redox-Coupled Proton Pumping in Proteins”, this Research Topic showcases how different experimental and computational approaches can be exploited to understand the molecular mechanisms of bioenergetic systems ( Wikström et al, 2023a ).…”

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confidence: 99%

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Editorial on the Research Topic Computational and experimental insights in proton and ion translocating bioenergetic systemsAs a follow up of the Research Topic-"Computational and Experimental Insights in Redox-Coupled Proton Pumping in Proteins", this Research Topic showcases how different experimental and computational approaches can be exploited to understand the molecular mechanisms of bioenergetic systems (Wikström et al, 2023a).Proteins performing redox-coupled proton pumping are key players in energy transduction in all living organisms. They are mostly observed in diverse respiratory chains (Marreiros et al, 2016;Calisto and Pereira, 2021).

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confidence: 99%