The mitochondrial respiratory chain from Rhodotorula mucilaginosa, an extremophile yeast

Castañeda-Tamez, Paulina; Chiquete-Félix, Natalia; Uribe-Carvajal, Salvador; Cabrera-Orefice, Alfredo

doi:10.1016/j.bbabio.2024.149035

Cited by 4 publications

(1 citation statement)

References 97 publications

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“…In the case of yeasts without selenium incorporation, the only molecular functions determined were in proteins related to oxidoreductase activity and catalytic activity, processes that were also identified in the study by [ 74 ], who observed a high antioxidant capacity and an efficient response to oxidative stress. These results could be governed by the ability of R. mucilaginosa 6S to synthesize carotenoids which are very effective antioxidants [ 32 , 75 ].…”

Section: Discussionmentioning

confidence: 99%

Enhancing Selenium Accumulation in Rhodotorula mucilaginosa Strain 6S Using a Proteomic Approach for Aquafeed Development

Díaz-Navarrete,

Sáez-Arteaga,

Marileo

et al. 2024

Biomolecules

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It is known that selenium (Se) is an essential trace element, important for the growth and other biological functions of fish. One of its most important functions is to contribute to the preservation of certain biological components, such as DNA, proteins, and lipids, providing protection against free radicals resulting from normal metabolism. The objective of this study was to evaluate and optimize selenium accumulation in the native yeast Rhodotorula mucilaginosa 6S. Sodium selenite was evaluated at different concentrations (5–10–15–20–30–40 mg/L). Similarly, the effects of different concentrations of nitrogen sources and pH on cell growth and selenium accumulation in the yeast were analyzed. Subsequently, the best cultivation conditions were scaled up to a 2 L reactor with constant aeration, and the proteome of the yeast cultured with and without sodium selenite was evaluated. The optimal conditions for biomass generation and selenium accumulation were found with ammonium chloride and pH 5.5. Incorporating sodium selenite (30 mg/L) during the exponential phase in the bioreactor after 72 h of cultivation resulted in 10 g/L of biomass, with 0.25 mg total Se/g biomass, composed of 25% proteins, 15% lipids, and 0.850 mg total carotenoids/g biomass. The analysis of the proteomes associated with yeast cultivation with and without selenium revealed a total of 1871 proteins. The results obtained showed that the dynamic changes in the proteome, in response to selenium in the experimental medium, are directly related to catalytic activity and oxidoreductase activity in the yeast. R. mucilaginosa 6S could be an alternative for the generation of selenium-rich biomass with a composition of other nutritional compounds also of interest in aquaculture, such as proteins, lipids, and pigments.

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Section: Discussionmentioning

confidence: 99%

Enhancing Selenium Accumulation in Rhodotorula mucilaginosa Strain 6S Using a Proteomic Approach for Aquafeed Development

Díaz-Navarrete,

Sáez-Arteaga,

Marileo

et al. 2024

Biomolecules

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Interactions between Root Endophytic Microorganisms and the Reduced Negative Ion Release Capacity of Phalaenopsis Aphrodite Rchb. F. Under High Temperature Stress

Ye,

Lv,

et al. 2024

Preprint

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Interactions between root endophytic microorganisms and the reduced negative ion release capacity of Phalaenopsis aphrodite Rchb. f. under high temperature stress

Ye,

Lv,

et al. 2024

Front. Plant Sci.

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IntroductionNegative oxygen ions are produced by plants through photosynthesis, utilizing "tip discharge" or the photoelectric effect, which has various functions such as sterilization, dust removal, and delaying aging. With global warming, high temperatures may affect the ability of Phalaenopsis aphrodite Rchb. f. to produce negative oxygen ions. P. aphrodite is commonly used in modern landscape planning and forest greening.MethodsIn this study, P. aphrodite was selected as the research object. By artificially simulating the climate, the control group (CK) and the high temperature stress group (HS) were set up in the experiment.ResultsThe study found that compared with the control group, the ability of P. aphrodite to produce negative oxygen ions significantly decreased when exposed to high temperature stress. Meanwhile, under high temperature stress treatment, peroxidase content increased by 102%, and proline content significantly increased by 35%.DiscussionRedundancy analysis results indicated a significant correlation between the root endophytic microbial community of P. aphrodite and negative oxygen ions, as well as physiological indicators. Under high temperature stress, P. aphrodite may affect the regulation of physiological indicators by modifying the composition of root endophytic microbial communities, thereby influencing the ability to release negative oxygen ions.

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The mitochondrial respiratory chain from Rhodotorula mucilaginosa, an extremophile yeast

Cited by 4 publications

References 97 publications

Enhancing Selenium Accumulation in Rhodotorula mucilaginosa Strain 6S Using a Proteomic Approach for Aquafeed Development

Enhancing Selenium Accumulation in Rhodotorula mucilaginosa Strain 6S Using a Proteomic Approach for Aquafeed Development

Interactions between Root Endophytic Microorganisms and the Reduced Negative Ion Release Capacity of Phalaenopsis Aphrodite Rchb. F. Under High Temperature Stress

Interactions between root endophytic microorganisms and the reduced negative ion release capacity of Phalaenopsis aphrodite Rchb. f. under high temperature stress

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