The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in <i>Schizosaccharomyces pombe</i>

Ray, Samriddha; Kume, Kazunori; Gupta, Sneha; Ge, Wanzhong; Balasubramanian, Mohan K.; Hirata, Dai; McCollum, Dannel

doi:10.1083/jcb.201002055

Cited by 44 publications

(84 citation statements)

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“…In S. pombe, the NDR-family kinase Mob2p-Orb6p (Hou et al 2003) is a component of the morphology network (MOR), which regulates polar growth in S. pombe. It has been suggested that increased SIN activity is antagonistic to the MOR (Ray et al 2010), and that SIN activity in mitosis turns off polar growth in preparation for cytokinesis; reviewed by Gupta and McCollum (2011). It is therefore possible that the morphology problems observed in the ypa2-D mutant may result from increased SIN activity, a direct effect upon the MOR, or a combination of these.…”

Section: Pp2a and Sin Signalingmentioning

confidence: 99%

Characterization of ypa1 and ypa2, the Schizosaccharomyces pombe Orthologs of the Peptidyl Proyl Isomerases That Activate PP2A, Reveals a Role for Ypa2p in the Regulation of Cytokinesis

Goyal

2012

Genetics

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The Schizosaccharomyces pombe septation initiation network (SIN) regulates cytokinesis. Cdc7p is the first kinase in the core SIN; we have screened genetically for SIN regulators by isolating cold-sensitive suppressors of cdc7-24. Our screen yielded a mutant in SPAC1782.05, one of the two fission yeast orthologs of mammalian phosphotyrosyl phosphatase activator. We have characterized this gene and its ortholog SPAC4F10.04, which we have named ypa2 and ypa1, respectively. We find that Ypa2p is the major form of protein phosphatase type 2A activator in S. pombe. A double ypa1-D ypa2-D null mutant is inviable, indicating that the two gene products have at least one essential overlapping function. Individually, the ypa1 and ypa2 genes are essential for survival only at low temperatures. The ypa2-D mutant divides at a reduced cell size and displays aberrant cell morphology and cytokinesis. Genetic analysis implicates Ypa2p as an inhibitor of the septation initiation network. We also isolated a cold-sensitive allele of ppa2, the major protein phosphatase type 2A catalytic subunit, implicating this enzyme as a regulator of the septation initiation network. SCHIZOSACCHAROMYCES pombe cells take the form of a cylinder capped by hemispherical ends. During interphase, cells grow mainly at their tips. Cell elongation ceases after mitotic commitment, and the cytoskeleton is reorganized in preparation for nuclear and cell division (reviewed by McCully and Robinow 1971;Mitchison and Nurse 1985;Marks et al. 1986;Hagan and Hyams 1988;Hagan 1998). The cell divides by fission after forming a septum in the middle of the cell. The site of division is defined at the onset of mitosis (Daga and Chang 2005), by the formation of an actomyosin contractile ring (CAR), whose assembly continues throughout mitosis (Wu et al. 2003;Pollard 2008;Pollard and Wu 2010). At the end of anaphase, the CAR is thought to guide synthesis of the multilayered division septum (reviewed by (Ishiguro 1998;Sipiczki 2007). Septation Initiation NetworkThe S. pombe septation initiation network (SIN) is a network of protein kinases that is essential for cytokinesis in the mitotic cycle; reviewed by (Gould and Simanis 1997;Simanis 2003;Wolfe and Gould 2005;Goyal et al. 2011). It is tightly regulated through the mitotic cell cycle to assure proper coordination of mitosis and cytokinesis, and during meiosis, where it is essential for spore formation (Krapp et al. 2006). Signaling requires the activity of three protein kinases, each of which has a regulatory subunit (kinase regulator); Cdc7p-Spg1p (Fankhauser and Simanis 1994;Schmidt et al. 1997;Mehta and Gould 2006), Sid1p-Cdc14p (Fankhauser and Simanis 1993;Guertin et al. 2000;Guertin and McCollum 2001), and Sid2p-Mob1p (Sparks et al. 1999;Hou et al. 2000;Salimova et al. 2000). SIN signaling is modulated by the nucleotide status of the GTPase Spg1p (Schmidt et al. 1997;Sohrmann et al. 1998), which is determined by the balance of spontaneous nucleotide exchange, a putative GEF, Etd1p Garcia-Cortes and McCollu...

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Section: Pp2a and Sin Signalingmentioning

confidence: 99%

Characterization of ypa1 and ypa2, the Schizosaccharomyces pombe Orthologs of the Peptidyl Proyl Isomerases That Activate PP2A, Reveals a Role for Ypa2p in the Regulation of Cytokinesis

Goyal

2012

Genetics

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“…Pmo25p, Nak1p and Sog2p localise to the SPB and the division site, whereas Mob2p, Orb6p and Mor2p only localise to the cell periphery and the division site. Orb6p and Nak1p activity is high in interphase and decreases during mitosis, when tip growth ceases (Kanai et al, 2005;Mendoza et al, 2005;Ray et al, 2010).…”

Section: Targets Of Sid2p In the Car Checkpointmentioning

confidence: 99%

“…Important for CAR assembly. Control of polarized growth Nak1p and Sog2p Gupta et al, 2014;Gupta et al, 2013;Ray et al, 2010 Proposed mutual inhibition between SIN and MOR prevents conflicts between polar growth and cytokinesis.…”

Section: Turning the Sin Offmentioning

confidence: 99%

Pombe's thirteen – control of fission yeast cell division by the septation initiation network

Simanis

2015

Journal of Cell Science

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The septation initiation network (SIN) regulates aspects of cell growth and division in Schizosaccharomyces pombe and is essential for cytokinesis. Insufficient signalling results in improper assembly of the contractile ring and failure of cytokinesis, generating multinucleated cells, whereas too much SIN signalling uncouples cytokinesis from the rest of the cell cycle. SIN signalling is therefore tightly controlled to coordinate cytokinesis with chromosome segregation. Signalling originates from the cytoplasmic face of the spindle pole body (SPB), and asymmetric localisation of some SIN proteins to one of the two SPBs during mitosis is important for regulation of the SIN. Recent studies have identified in vivo substrates of the SIN, which include components involved in mitotic control, those of the contractile ring and elements of the signalling pathway regulating polarised growth. The SIN is also required for spore formation following meiosis. This has provided insights into how the SIN performs its diverse functions in the cell cycle and shed new light on its regulation.

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“…However, there is no indication that significant numbers of dma1-D spores undergo inappropriate septation during the germination process (Krapp et al, 2010). Alternatively, the SIN might be silenced to prevent it from inhibiting the morphology network, which is required to establish polar growth (Gupta et al, 2013;Ray et al, 2010), in the germinating apolar spore. It is noteworthy that dma1-D spores are slow to germinate and resume polar growth (Krapp et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Dma1-dependent degradation of Septation Initiation Network proteins during meiosis inSchizosaccharomyces pombe

Krapp

2014

Journal of Cell Science

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The Schizosaccharomyces pombe septation initiation network (SIN) is required for cytokinesis during vegetative growth and for spore formation during meiosis. Regulation of the SIN during mitosis has been studied extensively, but less is known about its meiotic regulation. Here, we show that several aspects of SIN regulation differ between mitosis and meiosis. First, the presence of GTPbound Spg1p is not the main determinant of the timing of Cdc7p and Sid1p association with the spindle pole body (SPB) during meiosis. Second, the localisation dependencies of SIN proteins differ from those in mitotic cells, suggesting a modified functional organisation of the SIN during meiosis. Third, there is stage-specific degradation of SIN components in meiosis; Byr4p is degraded after meiosis I, whereas the degradation of Cdc7p, Cdc11p and Sid4p occurs after the second meiotic division and depends upon the ubiquitin ligase Dma1p. Finally, Dma1p-dependent degradation is not restricted to the SIN, as we show that Dma1p is needed for the degradation of Mcp6p (also known as Hrs1p) during meiosis I. Taken together, these data suggest that stage-specific targeted proteolysis plays an important role in regulating meiotic progression.

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The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in Schizosaccharomyces pombe

Abstract: The SIN pathway blocks inappropriate actin rearrangements during cytokinesis by preventing activation of the MOR pathway component Orb6.

Cited by 44 publications

References 50 publications

Characterization of ypa1 and ypa2, the Schizosaccharomyces pombe Orthologs of the Peptidyl Proyl Isomerases That Activate PP2A, Reveals a Role for Ypa2p in the Regulation of Cytokinesis

Characterization of ypa1 and ypa2, the Schizosaccharomyces pombe Orthologs of the Peptidyl Proyl Isomerases That Activate PP2A, Reveals a Role for Ypa2p in the Regulation of Cytokinesis

Pombe's thirteen – control of fission yeast cell division by the septation initiation network

Dma1-dependent degradation of Septation Initiation Network proteins during meiosis inSchizosaccharomyces pombe

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