The modification of an in vitro gas production test to 
detect roughage related differences in in vivo microbial 
protein synthesis as estimated by the excretion of 
purine derivatives

Blümmel, Michael; Mgomezulu, R.; Chen, X. B.; Makkar, H. P. S.; Becker, Kathrin; Ørskov, E. R.

doi:10.1017/s0021859699006991

Cited by 28 publications

(15 citation statements)

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“…The volume of gas produced after 24 h of incubation ranged from 163 for diets containing 100% of feed block (FB) to 214 ml · g −1 DM for diets without FB. These values are similar to those found for grass silage, oat-whole crop silage and maize stover leaves (200, 214 and 201 ml·g −1 DM, respectively) after 24 h of incubation (Blümmel et al, 1999;Gierus et al, 2008). The volume of gas produced from the fermented diet components is closely related to the digestibility and energy value for ruminants.…”

Section: Discussionsupporting

confidence: 84%

The effects of feed blocks containing tomato and cucumber by-products on in vitro ruminal fermentation, microbiota, and methane production

Romero-Huelva¹,

Martín-García²,

Nogales³

et al. 2013

J. Anim. Feed Sci.

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Section: Discussionsupporting

confidence: 84%

The effects of feed blocks containing tomato and cucumber by-products on in vitro ruminal fermentation, microbiota, and methane production

Romero-Huelva¹,

Martín-García²,

Nogales³

et al. 2013

J. Anim. Feed Sci.

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“…The greater the partition factor value, the greater the forage dry matter intake (Makkar, 2004) and lower the CH 4 production in ruminants (Blümmel et al, 1999). According to Makkar (2004), partition factor may vary from 2.74 to 4.41 mg of degraded OM mL -1 of gases produced.…”

Section: Discussionmentioning

confidence: 99%

Additives on in vitro ruminal fermentation characteristics of rice straw

et al. 2017

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-The objective of this study was to evaluate the effects of mineral and protein-energy (MPES), exogenous fibrolytic enzyme supplements (ES), combination of MPES + ES, and straw without supplement (WS) on digestibility, fermentation kinetic parameters, cumulative gas production, methane, CO 2 production, and volatile fatty acid concentration of rice straw of low and high nutritional value, estimated by in vitro techniques. The experimental design was randomized and factorial 2 × 4: two straws (low and high nutritional value) incubated with four supplements (MPES, ES, MPES + ES, and WS) and their interactions. Four experimental periods were used, totaling four replications per treatment over time. Data were analyzed by PROC MIXED of SAS. The in vitro dry matter and organic matter digestibilities of the rice straw with high nutritional value was improved by MPES, while the combination of MPES + ES supplements inhibited the digestibility of this straw. Dietary carbohydrate and nitrogen increased through MPES and MPES + ES supplements resulted in an increase in NH 3 -N concentration and a decrease in CO 2 production due to the microbial mass formation. However, this increase was not enough to improve organic matter degradability parameters, cummulative gas production, gas production kinetics, and acetate:propionate ratio and reduce methane emissions. The straw with high nutritional value showed greater content of nitrogen fraction a, effective degradability, cummulative gas production, and methane and CO 2 productions comparing with low-nutritional value straw. The use of MPES and MPES + ES supplements can be used as strategy to mitigate CO 2 in ruminant production systems that use rice straw.Key Words: digestibility, digestion kinetics, fatty acid, gas production, methane Revista Brasileira de Zootecnia

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“…Roughage with higher PF has been shown to have higher dry matter intake. Blümmel et al (1999) pointed out that different in vitro PF values are also reflected by in vivo microbial protein synthesis and in methane production by ruminants. These results show that the PF calculated in vitro provides meaningful information for predicting the dry matter intake, the microbial mass production in the rumen and the methane production of the whole ruminant animal.…”

Section: Methodsmentioning

confidence: 99%

In vitro fermentation of conventional diets commonly fed to dairy cows in Central Myanmar

Aung

Kyawt²,

Htun³

et al. 2016

jaar

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This study consisted of two experiments conducted to evaluate the effective net gas, fermentation kinetics (experiment 1), methane gas concentration, partitioning factor (PF) for microbial protein synthesis and in vitro dry matter digestibility (IVDMD) (experiment 2) of conventional diets commonly fed to dairy cows in Central Myanmar. The conventional diets from four areas [diet from Sin Tel area (Diet-ST), diet from Myay Nguarea (Diet-MN), diet from Ta Pelarea (Diet-TP) and diet from Amarapuraarea (Diet-AM)] were used as experimental diets in this study. In most of conventional diets, rice straw, sorghum stover and natural grass were used as roughage source and cotton seed cake and broken rice were used as concentrate. However in some diets, sesame residue and butter bean residue were used as roughage source instead of sorghum stover and natural grass. The roughage to concentrate ratio and crude protein (CP) content of conventional diets ranged from 53:47 to 72:28 and 11.46 to 17.96%, respectively. In experiment 1,the effective net gas volume of Diet-TP was lower (p<0.05) than Diet-ST and Diet-AM and generally, the fermentation kinetics (a, b, c and a+b) of Diet-TP were also lower than those of other diets. In the experiment 2, the lower value (p<0.05) of short chain fatty acid (SCFA) was found in Diet-MN and Diet-TP while the higher values (p<0.05) of metabolizable energy (ME) and organic matter digestibility (OMD) were observed Diet-AM and Diet-TP. Although methane gas concentration of Diet-TP was higher(p<0.05) than those of other diets, the greater values of IVDMD and PF were observed in Diet-TP. According to these findings, it was perceived that all conventional diets have different nutritional qualities which are useful for production and health of dairy cows; however the Diet-TP possessed the highest nutritional qualities among the conventional diets.

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The modification of an in vitro gas production test to detect roughage related differences in in vivo microbial protein synthesis as estimated by the excretion of purine derivatives

Cited by 28 publications

References 0 publications

The effects of feed blocks containing tomato and cucumber by-products on <i>in vitro</i> ruminal fermentation, microbiota, and methane production

The effects of feed blocks containing tomato and cucumber by-products on <i>in vitro</i> ruminal fermentation, microbiota, and methane production

Additives on in vitro ruminal fermentation characteristics of rice straw

In vitro fermentation of conventional diets commonly fed to dairy cows in Central Myanmar

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