The modified HET-CAM as a model for the assessment of the inflammatory response to tissue tolerable plasma

Bender, Claudia; Partecke, Lars-Ivo; Kindel, E.; Döring, Friederike; Lademann, Jürgen; Heidecke, Claus–Dieter; Krämer, Axel; Hübner, Nils–Olaf

doi:10.1016/j.tiv.2010.11.012

Cited by 41 publications

(28 citation statements)

References 43 publications

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“…Hence, this read-out system may have limitations and future studies should collect the remaining liquid on the egg’s membrane or other egg material after plasma treatment to find markers indicative for ROS-stress. Yet, and assuming a treatment area of 2 cm 2 , it should be stressed that the long exposure times used in this study exceeded the therapeutically effective duration for the kINPen 09 (5 s per cm 2 ) [49] 15 fold and for the kINPen MED (30–60 s per cm 2 ) [46] 5–10 fold. As these treatment regimens did not cause significant genotoxicity they suggest the kINPen plasma not to be a mutagenic hazard within such treatment times.…”

Section: Discussionmentioning

confidence: 99%

“…A 72 h contact time was allowed for all substances because it yields an high MNE II-rate, a low toxicity, and a high xenobiotic metabolisms [41, 47, 48]. Moreover, we wanted to investigate the long-term effects of plasma by identifying possible mutagenic effects of a single plasma exposure regimen which exceeded the recommended application time by far [1, 46, 49]. Prior to plasma exposure (Fig 1B) and to reduce dehydration, 100 μl of 0.9% NaCl solution was applied.…”

Section: Methodsmentioning

confidence: 99%

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Investigating the Mutagenicity of a Cold Argon-Plasma Jet in an HET-MN Model

et al. 2016

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ObjectiveSo-called cold physical plasmas for biomedical applications generate reactive oxygen and nitrogen species and the latter can trigger DNA damage at high concentrations. Therefore, the mutagenic risks of a certified atmospheric pressure argon plasma jet (kINPen MED) and its predecessor model (kINPen 09) were assessed.MethodsInner egg membranes of fertilized chicken eggs received a single treatment with either the kINPen 09 (1.5, 2.0, or 2.5 min) or the kINPen MED (3, 4, 5, or 10 min). After three days of incubation, blood smears (panoptic May-Grünwald-Giemsa stain) were performed, and 1000 erythrocytes per egg were evaluated for the presence of polychromatic and normochromic nuclear staining as well as nuclear aberrations and binucleated cells (hen’s egg test for micronuclei induction, HET-MN). At the same time, the embryo mortality was documented. For each experiment, positive controls (cyclophosphamide and methotrexate) and negative controls (NaCl-solution, argon gas) were included. Additionally, the antioxidant potential of the blood plasma was assessed by ascorbic acid oxidation assay after treatment.ResultsFor both plasma sources, there was no evidence of genotoxicity, although at the longest plasma exposure time of 10 min the mortality of the embryos exceeded 40%. The antioxidant potential in the egg’s blood plasma was not significantly reduced immediately (p = 0.32) or 1 h (p = 0.19) post exposure to cold plasma.ConclusionThe longest plasma treatment time with the kINPen MED was 5–10 fold above the recommended limit for treatment of chronic wounds in clinics. We did not find mutagenic effects for any plasma treatment time using the either kINPen 09 or kINPen MED. The data provided with the current study seem to confirm the lack of a genotoxic potential suggesting that a veterinary or clinical application of these argon plasma jets does not pose mutagenic risks.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Investigating the Mutagenicity of a Cold Argon-Plasma Jet in an HET-MN Model

et al. 2016

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“…An increased IL-6 and IL-8 release was induced for keratinocytes and mononuclear cells. The support of circulation and angiogenesis was also observed [262]. On the chorioallantoic membrane, a heightened leukocyte-endothelium interaction with an increased fraction of rolling leukocytes and leukocytes solidly attached to the vascular endothelium (as a precursor to diapedesis into the surrounding tissue) was documented [247,263].…”

Section: Future Perspectivesmentioning

confidence: 99%

Consensus on Wound Antisepsis: Update 2018

Krämer

Dissemond²,

Kim³

et al. 2017

Skin Pharmacol Physiol

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Wound antisepsis has undergone a renaissance due to the introduction of highly effective wound-compatible antimicrobial agents and the spread of multidrug-resistant organisms (MDROs). However, a strict indication must be set for the application of these agents. An infected or critically colonized wound must be treated antiseptically. In addition, systemic antibiotic therapy is required in case the infection spreads. If applied preventively, the Wounds-at-Risk Score allows an assessment of the risk for infection and thus appropriateness of the indication. The content of this updated consensus recommendation still largely consists of discussing properties of octenidine dihydrochloride (OCT), polihexanide, and iodophores. The evaluations of hypochlorite, taurolidine, and silver ions have been updated. For critically colonized and infected chronic wounds as well as for burns, polihexanide is classified as the active agent of choice. The combination 0.1% OCT/phenoxyethanol (PE) solution is suitable for acute, contaminated, and traumatic wounds, including MRSA-colonized wounds due to its deep action. For chronic wounds, preparations with 0.05% OCT are preferable. For bite, stab/puncture, and gunshot wounds, polyvinylpyrrolidone (PVP)-iodine is the first choice, while polihexanide and hypochlorite are superior to PVP-iodine for the treatment of contaminated acute and chronic wounds. For the decolonization of wounds colonized or infected with MDROs, the combination of OCT/PE is preferred. For peritoneal rinsing or rinsing of other cavities with a lack of drainage potential as well as the risk of central nervous system exposure, hypochlorite is the superior active agent. Silver-sulfadiazine is classified as dispensable, while dyes, organic mercury compounds, and hydrogen peroxide alone are classified as obsolete. As promising prospects, acetic acid, the combination of negative pressure wound therapy with the instillation of antiseptics (NPWTi), and cold atmospheric plasma are also subjects of this assessment.

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“…CAP has been investigated in the treatment of chronic wounds [3,4], including chronic ulcers without [5] and in combination with the antiseptic octenidine [6] (which increased the efficacy of CAP due to its residual antimicrobial effect [7]), on skin graft donor wounds [8], for skin antisepsis [9,10,11,12], and for the inactivation of microorganisms embedded in biofilms [13,14,15,16,17]. …”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Cold Atmospheric Pressure Argon Plasma against 78 Genetically Different (mecA, luk-P, agr or Capsular Polysaccharide Type) Staphylococcus aureus Strains

Matthes

Lührman

Holtfreter³

et al. 2016

Skin Pharmacol Physiol

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Previous studies on the antimicrobial activity of cold atmospheric pressure argon plasma showed varying effects against mecA⁺ or mecA^-Staphylococcus aureus strains. This observation may have important clinical and epidemiological implications. Here, the antibacterial activity of argon plasma was investigated against 78 genetically different S. aureus strains, stratified by mecA, luk-P, agr1-4, or the cell wall capsule polysaccharide types 5 and 8. kINPen09® served as the plasma source for all experiments. On agar plates, mecA⁺luk-P^-S. aureus strains showed a decreased susceptibility against plasma compared to other S. aureus strains. This study underlines the high complexity of microbial defence against antimicrobial treatment and confirms a previously reported strain-dependent susceptibility of S. aureus to plasma treatment.

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The modified HET-CAM as a model for the assessment of the inflammatory response to tissue tolerable plasma

Cited by 41 publications

References 43 publications

Investigating the Mutagenicity of a Cold Argon-Plasma Jet in an HET-MN Model

Investigating the Mutagenicity of a Cold Argon-Plasma Jet in an HET-MN Model

Consensus on Wound Antisepsis: Update 2018

Antibacterial Activity of Cold Atmospheric Pressure Argon Plasma against 78 Genetically Different (<b><i>mecA</i></b>, <b><i>luk-P</i></b>, <b><i>agr</i></b> or Capsular Polysaccharide Type) <b><i>Staphylococcus aureus </i></b>Strains

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