The modulating effect of lipid bilayer/p-coumaric acid interactions on electrical properties of model lipid membranes and human glioblastoma cells

Naumowicz, Monika; Kusaczuk, Magdalena; Kruszewski, Marcin Andrzej; Gál, Miroslav; Krętowski, Rafał; Cechowska-Pasko, Marzanna; Kotyńska, Joanna

doi:10.1016/j.bioorg.2019.103242

Cited by 11 publications

(16 citation statements)

References 53 publications

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“…According to the available data, specific interactions of polyphenols with negatively charged phospholipids rather than zwitterionic ones are more likely to appear in cell membranes [39]. This is in line with our previous results [23], where phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine liposomal surfaces were examined and the specific interactions of p-CoA with phosphatidylserine, which has a net of negative charge at physiological pH, were detected. Detection of these interactions is very valuable information, because phosphatidylserine exposition to the outer membrane surface, resulting in additional negatively charged groups, is the most pronounced effect of the lipid asymmetry disorders, the maintenance of which is extremely important for many cellular processes.…”

Section: Discussionsupporting

confidence: 89%

“…These concentrations correspond with high cytotoxic effect of p-CoA in all cell lines. The results of MTT analysis performed for T98G cells are presented in Figure 1, and cytotoxicity results for LN-18, LN-229, and LBC3 cells were described in our previous paper [23]. In all cases, untreated cells served as a control.…”

Section: Resultsmentioning

confidence: 95%

“…The experimental data concerning surface charge of membranes of LN-18, LN-229, and LBC3 glioblastoma cell lines treated with p-CoA are available in [23]. Herein, we present the experimental values determined for one additional GBM cell line T98G.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous article [23], the systematic studies investigating the effect of p-CoA on electrical properties of biological membrane models and the membranes of living cells were presented. Model membranes were made of three phospholipids asymmetrically distributed in the plasma membrane: Phosphatidylserine (PS)—the most abundant anionic phospholipid of the plasma membrane, which is tightly segregated to the internal leaflet of the plasma membrane in most cell types; phosphatidylethanolamine (PE)—neutral phospholipid also situated predominately in the internal leaflet; and phosphatidylcholine (PC)—neutral phospholipid, which appear to be mainly situated in the external leaflet.…”

Section: Introductionmentioning

confidence: 99%

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The Modulating Effect of p-Coumaric Acid on The Surface Charge Density of Human Glioblastoma Cell Membranes

Kruszewski

Kotyńska

Kusaczuk

et al. 2019

IJMS

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p-Coumaric acid (p-CoA), a phenolic acid belonging to the hydroxycinnamic acids family, is a compound with tentative anticancer potential. Microelectrophoretic mobility measurements conducted at various pH values of electrolyte solution were applied to study p-CoA effects on electrical properties of human glioblastoma cell membranes. The obtained results demonstrated that after the p-CoA treatment, the surface charge density of cancer cells changed in alkaline pH solutions, while no noticeable changes were observed in cell membranes incubated with p-CoA compared to control at acidic pH solutions. A four-equilibrium model was used to describe the phenomena occurring on the cell membrane surface. The total surface concentrations of both acidic and basic functional groups and their association constants with solution ions were calculated and used to define theoretical curves of membrane surface charge density versus pH. The resulting theoretical curves and the experimental data were compared to verify the reliability and validity of the adopted model. The deviation of both kinds of data obtained at a higher pH may be caused by disregarding interactions between the functional groups of cancer cells. Processes occurring in the cell membranes after their incubation with p-CoA can lead to disorders of existing equilibria, which result in changes in values of the parameters describing these equilibria.

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Section: Discussionsupporting

confidence: 89%

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Modulating Effect of p-Coumaric Acid on The Surface Charge Density of Human Glioblastoma Cell Membranes

Kruszewski

Kotyńska

Kusaczuk

et al. 2019

IJMS

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“…One of the techniques that might be used to estimate zeta potential is the electrophoretic light scattering (ELS) technique based on dynamic light scattering (DLS), in which the shift in the frequency or in the oscillation phase of the laser beam depends on the mobility of particles/cells in an alternating electric field [ 14 ]. In this respect, in our previous works, we have already applied the ELS technique to study human glioblastoma cells treated with either silica nanoparticles [ 14 , 15 ] or phenolic compounds such as quercitin [ 16 ] and p-coumaric acid [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Monitoring of the Surface Charge Density Changes of Human Glioblastoma Cell Membranes upon Cinnamic and Ferulic Acids Treatment

Naumowicz

Kusaczuk

Zając

et al. 2020

IJMS

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Cinnamic acid (CA) and ferulic acid (FA) are naturally occurring phenolic acids claimed to exert beneficial effects against disorders related to oxidative stress, including cancer. One such malignancy that still remains a therapeutic challenge mainly due to its heterogeneity and inaccessibility to therapeutic agents is Glioblastoma multiforme (GBM). Here, the influence of CA and FA on the surface charge density of human GBM cell line LN-229 was studied using the electrophoretic light scattering technique. Also, the cytotoxicity of both phenolic acids was determined by metabolic activity-assessing tetrazolium test (MTT) analysis after exposure to CA and FA for 24 h and 48 h. Results showed that both compounds reduced cell viability of LN-229 cells, with more pronounced effect evoked by CA as reflected in IC50 values. Further analyses demonstrated that, after treatment with both phenolic acids, the negative charge of membranes decreased at high pH values and the positive charge of the membranes increased at low pH values compared to the data obtained for untreated cells. Afterward, a four-equilibrium model was applied to estimate the total surface concentrations of both acidic and basic functional groups and their association constants with solution ions in order to calculate theoretical values of membrane surface charge densities. Then, the theoretical data were compared to the experimental data in order to verify the mathematical model. As such, our results indicate that application of electrochemical methods to determine specific drug–membrane interactions might be crucial for predicting their pharmacological activity and bioavailability.

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Chemoprevention and therapeutic role of essential oils and phenolic compounds: Modeling tumor microenvironment in glioblastoma

Magalhães

Manadas

Efferth

et al. 2021

Pharmacological Research

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The modulating effect of lipid bilayer/p-coumaric acid interactions on electrical properties of model lipid membranes and human glioblastoma cells

Cited by 11 publications

References 53 publications

The Modulating Effect of p-Coumaric Acid on The Surface Charge Density of Human Glioblastoma Cell Membranes

The Modulating Effect of p-Coumaric Acid on The Surface Charge Density of Human Glioblastoma Cell Membranes

Monitoring of the Surface Charge Density Changes of Human Glioblastoma Cell Membranes upon Cinnamic and Ferulic Acids Treatment

Chemoprevention and therapeutic role of essential oils and phenolic compounds: Modeling tumor microenvironment in glioblastoma

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