The modulation of IL-2 dependent proliferation of CTLL-2 cells by 2-methyl-thiazolidine-2,4-dicarboxylic acid

Wtodek, L.; Grabowska, Agnieszka; Marcinkiewicz, Janusz

doi:10.1016/0162-3109(95)00004-d

Cited by 5 publications

(1 citation statement)

References 27 publications

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“…They were resuspended in growth medium without IL-2 to 5i 10 5 cells mL − 1 . Fifty microliter of cell suspension was placed into each well of a 96-well tissue culture plate and incubated in the presence of syringin and control drugs (dexamethasone, indometacin and A77,1726) and 25 IU mL − 1 of IL-2 for 48 h (Wlodek et al 1995).…”

Section: Cd4j T Lymphocyte Proliferation Assaymentioning

confidence: 99%

In-vitro and in-vivo immunomodulatory effects of syringin

Cho

Nam

Kim

et al. 2001

Journal of Pharmacy and Pharmacology

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Syringin was found to possess immunomodulatory activity by which it inhibited the in-vitro immunohaemolysis of antibody-coated sheep erythrocytes by guinea-pig serum through suppression of C3-convertase of the classical complement. In this study, we examined its in-vitro and in-vivo activity on tumour necrosis factor (TNF)-alpha and nitric oxide (NO) production, CD4+ T cell and CD8+ cytotoxic T cell (CTLL-2) proliferation, and croton oil-, arachidonic acid- and fluorescein-isothiocynate (FITC)-induced mouse ear oedema model. Syringin significantly inhibited both TNF-alpha production from lipopolysaccharide (LPS)-stimulated RAW264.7 cells and CD8+ T cell (CTLL-2) proliferation in a dose-dependent manner, whereas neither NO production nor CD4+ T cell proliferation were blocked even by high concentrations of syringin. In the invivo experiments, syringin also significantly suppressed FITC-induced ear oedema in mice but not the ear oedema induced by croton or arachidonic acid. These results suggest that syringin may be implicated as an immunomodulator having an anti-allergic effect rather than an anti-inflammatory effect. The anti-allergic effect of syringin seems to be due, in part, to inhibition of TNF-alpha production and cytotoxic T cell proliferation.

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Section: Cd4j T Lymphocyte Proliferation Assaymentioning

confidence: 99%