The morphological and biochemical characteristics of the consecutive ejaculates from Il-de-France rams at various ages

Abstract

:In the agricultural practice one ram is used for the insemination a lot of ewes according to the breeding plan. For the insurance of the successful insemination of ewes reached the heat simultaneously, it is necessary to obtain some consistently ejaculates from one ram. The aim of the present work was the estimation of the sperm quality in consecutive ejaculates from Il-de-France rams at various ages. It was investigated over 40 ejaculates from 5 rams of the IAS-Kostinbrod herd between 1, 5 and 4 years old during the breeding season. The sperm was collected by artificial vagina. It was estimated the following morphological and biochemical parameters: volume, count of the spermatozoids in 1ml, motility, survivability at 39ºC and activity of the alkaline phosphatase in the semen plasma. In the consecutive ejaculates of both groups no significant morphological changes of spermatozoids were established. The obtained results are shown that the concentration of the spermatozoids and the activity of the alkaline phosphatase in the semen plasma reduce in the third consistently ejaculate, but the keeping of Aph activity in second ejaculates despite decreasing of the sperm volume and concentration of spermatozoid .On the base of these results we could give reference for use of the first and second ejaculates in the practical insemination of ewes.

Effect of ejaculation frequency on ram semen characteristics, seminal plasma composition and chilled sperm quality

“…In dissimilarity, in Gulf Coast Native rams, the second ejaculate had greater motility than the first one, collected at a 10-minute interval (Nel-Themaat et al, 2006). There was a negative correlation between sperm volume and sperm concentration, as well as enhanced motility in the third ejaculate following three consecutive 30-minute collections from Ile de France rams (Kistanova, et al, 2007). Besides, seminal plasma composition can be affected by collection frequency (Kaya et al, 2002) which can affect, in turn the preserved sperm quality (Ramírez-Vasquez et al, 2019).…”