The morphology and fine structure of the larval midgut of a moth (Manduca sexta) in relation to active ion transport

Cioffi, Moira

doi:10.1016/0040-8166(79)90057-0

Cited by 179 publications

(79 citation statements)

References 17 publications

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“…The general morphology of midgut epithelium in larvae of D. saccharalis is similar to that described for many Lepidoptera such as Manduca sexta L. (Cioffi 1979) e Erynnis ello L. (Santos et al 1984) (Sphingidae), Spodoptera frugiperda Smith (Jordão et al 1999) and Anticarsia gemmatalis Hübner (Levy et al 2004) (Noctuidae), among many other species (for a review, see Lehane & Billingsley 1996).…”

Section: Discussionmentioning

confidence: 63%

“…In D. saccharalis larvae differences in the morphology of this cavity were not consistent in LM; however, SEM analyses show that, besides this enlargement of the chamber from the anterior to the posterior region, there even occurs an apparent increase in the number of chamber's cytoplasm extrusion with mitochondria throughout its extension that may be responsible for ionic transport. In D. saccharalis preparations it becomes clear that the chamber, in cells of the posterior region, reach the base of the cell, differently from other Lepidoptera (Cioffi 1979, Terra 1988. The major diameter of chamber's cytoplasm extrusion at the chamber base may be explained by the presence of mitochondria inside these cytoplasmic projections, as described in innumerable insects (Anderson & Harvey 1966, Lehane & Billingsley 1996, Cavalcante & Cruz-Landim 1999, Levy et al 2004.…”

Section: Discussionmentioning

confidence: 99%

“…The format of the goblet cell chamber has been studied in other Lepidoptera, where it was indicated that the apical portion of this cavity is more enlarged in cells of the posterior midgut region (Cioffi 1979, Santos et al 1984, Jordão et al 1999. In D. saccharalis larvae differences in the morphology of this cavity were not consistent in LM; however, SEM analyses show that, besides this enlargement of the chamber from the anterior to the posterior region, there even occurs an apparent increase in the number of chamber's cytoplasm extrusion with mitochondria throughout its extension that may be responsible for ionic transport.…”

Section: Discussionmentioning

confidence: 99%

“…Many of the works referencing epithelial cells correlate their morphology with possible functions (Cioffi 1979, Santos et al 1984.…”

mentioning

confidence: 99%

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Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae

Pinheiro¹,

Quagio-Grassiotto²,

Gregório³

2008

Neotrop. entomol.

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Diferenças Morfológicas Regionais das Células Epiteliais ao Longo do Intestino Médio de Larvas deDiatraea saccharalis Fabricius (Lepidoptera: Crambidae)RESUMO -A broca da cana, Diatraea saccharalis Fabricius, é uma praga da cana-de-açúcar e outras plantações. O objetivo deste trabalho foi caracterizar variações morfológicas nas células epiteliais (colunares, caliciformes e regenerativas) ao longo do intestino médio de larvas de D. saccharalis. Fragmentos do intestino médio (anterior, mediano e posterior) foram fi xados e processados para microscopia de luz e eletrônica de varredura. Existem diferenças morfológicas citoquímicas e ultraestruturais nas células epiteliais, dependendo da sua localização no intestino médio. A superfície apical de algumas células colunares exibe projeções citoplasmáticas que aumentam em número e volume da região anterior para a posterior do intestino médio. Existe aumento dos grânulos PAS-positivos (Reação de Schiff) no citoplasma apical das células colunares e regenerativas, da região anterior para a posterior. A câmara das células caliciformes, na região anterior do intestino médio, mostra seu ápice estreito, enquanto que na posterior essa porção da câmara é alargada; as evaginações citoplasmáticas da câmara são pequenas e fi nas no ápice, sendo numerosas, longas e mais espessas na porção basal. Os resultados sugerem que o intestino médio da broca da cana apresenta duas regiões morfologicamente distintas, a anterior e posterior; a região mediana é uma região de transição.PALAVRAS-CHAVE: Morfologia, broca da cana, histoquímica, microscopia eletrônica de varredura, microscopia de luz ABSTRACT -The sugarcane borer, Diatraea saccharalis Fabricius, is a pest to sugarcane and many other crops. This work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of D. saccharalis larvae. Fragments of the midgut (anterior, middle and posterior regions) were fi xed and processed by light and scanning electron microscopy. There are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depending on their localization along the midgut. The apical surface of columnar cells shows an increase in both number and size of the apical protrusions from the anterior to the posterior midgut regions.There is an increase in the amount of PAS-positive (Periodic Acid-Schiff Reaction) granules detected in the cytoplasm of both the columnar and regenerative cells, from the anterior to the posterior region. The goblet cell apical surface is narrow in the anterior region, and enlarged in the posterior midgut; the chamber's cytoplasm extrusion are small and thin at the apical cavity surface, being thicker, longer and more numerous at the basal portion of the cavity. Our results suggest that the sugarcane borer midgut has two morphologically different regions, the anterior and the posterior; the middle region is a transitional region.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Many of the works referencing epithelial cells correlate their morphology with possible functions (Cioffi 1979, Santos et al 1984.…”

mentioning

confidence: 99%

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Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae

Pinheiro¹,

Quagio-Grassiotto²,

Gregório³

2008

Neotrop. entomol.

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“…When the toxin dosage fails to kill the insect, its cells are substituted allowing normal feeding to proceed and the recuperation of development of the insect (Spies and Spence, 1995). The midgut of the Lepidoptera is composed of pseudostratified epithelium, formed by three kinds of cells -columnar, globular (Cioffi, 1979) and regenerative -located on the base, between the columnar and the globular cells. These cells demonstrate mitosis activity before each change (Baldwin and Hakim, 1991;Engelhard et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Histopathology and the lethal effect of Cry proteins and strains of Bacillus thuringiensis Berliner in Spodoptera frugiperda J.E. Smith Caterpillars (Lepidoptera, Noctuidae)

Knaak

Franz²,

Santos

et al. 2010

Braz. J. Biol.

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Among the phytophagous insects which attack crops, the fall armyworm, Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera, Noctuidae) is particularly harmful in the initial growth phase of rice plants. As a potential means of controlling this pest, and considering that the entomopathogen Bacillus thuringiensis Berliner demonstrates toxicity due to synthesis of the Cry protein, the present study was undertaken to evaluate this toxic effect of B. thuringiensis thuringiensis 407 (pH 408) and B. thuringiensis kurstaki HD-73 on S. frugiperda. The following method was used. Both bacterial strains were evaluated in vitro in 1 st instar S. frugiperda caterpillars, by means of histopathological assays. The Cry1Ab and Cry1Ac proteins, codified by the respective strains of B. thuringiensis, were evaluated in vivo by bioassays of 1 st instar S. frugiperda caterpillars in order to determine the Mean Lethal Concentration (LC 50 ). The results of the histopathological analysis of the midget of S. frugiperda caterpillars demonstrate that treatment with the B. thuringiensis thuringiensis strain was more efficient, because the degradations of the microvilosities started 9 hours after treatment application (HAT), while in the B. thuringiensis kurstaki the same effect was noticed only after 12 HAT. Toxicity data of the Cry1Ab and Cry1Ac proteins presented for the target-species LC 50 levels of 9.29 and 1.79 µg.cm , respectivamente. As cepas e proteínas sintetizadas por B. thuringiensis thuringiensis e B. thuringiensis kurstaki são eficientes no controle de S. frugiperda, e poderão ser usadas na produção de novos biopesticidas ou a utilização dos genes na transformação genética de Oryza sativa L. Histopatologia e efeito letal de cepas e proteínas Cry de Bacillus thuringiensis

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Modification of the nutritional parameters and of midgut biochemical and absorptive functions induced by the IGR fenoxycarb inBombyx mori larvae

Leonardi

Cappellozza

Eguileor

et al. 1998

Arch. Insect Biochem. Physiol.

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The morphology and fine structure of the larval midgut of a moth (Manduca sexta) in relation to active ion transport

Cited by 179 publications

References 17 publications

Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae

Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae

Histopathology and the lethal effect of Cry proteins and strains of Bacillus thuringiensis Berliner in Spodoptera frugiperda J.E. Smith Caterpillars (Lepidoptera, Noctuidae)

Modification of the nutritional parameters and of midgut biochemical and absorptive functions induced by the IGR fenoxycarb inBombyx mori larvae

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