The Mouse Ovarian Surface Epithelium Contains a Population of LY6A (SCA-1) Expressing Progenitor Cells That Are Regulated by Ovulation-Associated Factors1

Abstract

The ovarian surface epithelium, a single layer of poorly differentiated epithelial cells, covers the surface of the ovary and is ruptured during ovulation. Little is known about the changes that occur in this layer before or during ovulation, and even less is known about the regenerative processes that occur after the surface is ruptured to release a mature oocyte. Recently, a population of mouse ovarian surface epithelial (MOSE) cells that exhibit progenitor/stem cell characteristics has been identified, though neither a genetic marker nor how these cells are regulated has been determined. We have identified a defined population of MOSE cells with progenitor cell characteristics that express the stem cell marker lymphocyte antigen 6 complex, locus A (LY6A; also known as stem cell antigen-1 [SCA-1]). By testing the effect of factors found in the follicular fluid at ovulation on proliferation, sphere formation, and LY6A expression, we have determined that the size of the LY6A-expressing (LY6A+) progenitor cell population is regulated by at least two ovulation-associated factors present in the follicular fluid: transforming growth factor beta 1 and leukemia-inhibitory factor. Our work has identified a population of LY6A+ MOSE progenitor cells on the surface of the ovary that may play a role in ovulatory wound healing.

Epithelial ovarian cancer stem cells: underlying complexity of a simple paradigm

“…Gamwell et al (2012) have examined candidate markers in the SP fraction of cultures of murine OSE. SP cells derived from murine OSE were enriched in the stem cell markers LY6A and CD117 .…”

Fat-associated lymphoid cluster in Cyprinus carpio: Characterisation and its relation with peritoneal haemangiosarcoma

“…C-kit of the common carp was related to c-kit of F. Poeciliidae fish species, with predicted v-kit (mast/stem cell growth factor receptor "Kit") of P. formosa (NW_006799976.1) and with a-kit involved in the development of adult melanophores in fish [22]. Despite the lack of available information, molecular phylogenetic analysis demonstrates the existence of a very primitive undifferentiated marker in lymphoid stem cells of fish species able to promote the differentiation of these cells, as documented in mammals [26,41]. Although there are no concrete reports about phylogeny of c-kit in fish, it was presupposed that the last common ancestor of hagfish, lamprey, shark 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 and teleost fish able to express c-kit dates to about 450e500 million years ago [15,21].…”

“…FALC cells express c-kit [47], a tyrosine-protein kinase that acts as a cell-surface receptor for KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, haematopoiesis and stem cell maintenance [7,69]. These cells express Sca-1 or Ly6A (lymphocyte antigen 6 complex, locus A), which is also known as stem cell antigen-1 [26]. The lymphocyte 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 antigen 6 complex is a protein-coding gene associated with subvalvular aortic stenosis, and with acute promyelocytic leukaemic diseases [10].…”

Gonadotropins Activate Oncogenic Pathways to Enhance Proliferation in Normal Mouse Ovarian Surface Epithelium

“…FSH at all three doses in 3D significantly increased proliferation of OSE as compared to basal media, while LH and the combination of FSH and LH only significantly increased proliferation at 10 and 100 mIU/mL (Figure 1a). In order to compare the effects of FSH, LH and FSH+LH on OSE proliferation in vitro , 2D mouse ovarian surface epithelial cells (MOSE) were analyzed for proliferation after stimulation with gonadotropins for 8 days [29]. FSH at 1, 10 and 100 mIU/mL increased proliferation above basal levels, LH increased proliferation at 1 and 10 mIU/mL, and FSH+LH increased proliferation at 10 and 100 mIU/mL (Figure 1b).…”