The Mouthwash: A Non‐Invasive Sampling Method to Study Cytokine Gene Polymorphisms

Laine, ML; Farré, Maria A.; Crusius, J.B.A.; Winkelhoff, A.J. van; Peña, Amado Salvador

doi:10.1902/jop.2000.71.8.1315

Cited by 41 publications

(26 citation statements)

References 22 publications

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“…Bacterial DNA was extracted from saliva and subgingival biofilm samples according to the methodology described by Laine et al (2000). Briefly, samples were centrifuged at 300 × g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection

Souto¹,

Silva-Boghossian²,

Colombo³

2014

Braz. J. Microbiol.

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P. aeruginosa and Acinetobacter spp. are important pathogens associated with late nosocomial pneumonia in hospitalized and institutionalized individuals. The oral cavity may be a major source of these respiratory pathogens, particularly in the presence of poor oral hygiene and periodontal infection. This study investigated the prevalence of P. aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with periodontal disease or health. Samples were obtained from 55 periodontally healthy (PH) and 169 chronic periodontitis (CP) patients. DNA was obtained from the samples and detection of P. aeruginosa and Acinetobacter spp. was carried out by multiplex and nested PCR. P. aeruginosa and Acinetobacter spp. were detected in 40% and 45% of all samples, respectively. No significant differences in the distribution of these microorganisms between men and women, subgingival biofilm and saliva samples, patients ≤ 35 and > 35 years of age, and smokers and non-smokers were observed regardless periodontal status (p > 0.05). In contrast, the frequencies of P. aeruginosa and Acinetobacter spp. in saliva and biofilm samples were significantly greater in CP than PH patients (p < 0.01). Smokers presenting P. aeruginosa and high frequencies of supragingival plaque were more likely to present CP than PH. P. aeruginosa and Acinetobacter spp. are frequently detected in the oral microbiota of CP. Poor oral hygiene, smoking and the presence of P. aeruginosa are strongly associated with periodontitis.

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Section: Methodsmentioning

confidence: 99%

Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection

Souto¹,

Silva-Boghossian²,

Colombo³

2014

Braz. J. Microbiol.

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“…Genomic DNA was extracted from blood (Invisorb Blood Universal Kit, Invitek, Berlin, Germany) or mouthwash samples,37 and was amplified by whole genome amplification (GenomiPhi, Amersham, Uppsala, Sweden). TagSNPs were selected from the CEU data of the International HapMap project (http://www.hapmap.org, NCBI build 36, 2008-03) using the Tagger feature38 of Haploview 4.0 39.…”

Section: Methodsmentioning

confidence: 99%

CDKN2BAS is associated with periodontitis in different European populations and is activated by bacterial infection

Schäefer

Richter

Dommisch

et al. 2010

Journal of Medical Genetics

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“…Sampling and DNA extraction procedures were performed according to Laine et al (32). Briefly, each individual rinsed out his/her mouth with 10 mL of sterile 0.9% saline solution for 1 minute.…”

Section: Sample Taking and Dna Extractionmentioning

confidence: 99%

Relationship Between Fcγ Receptor and Interleukin-1 Gene Polymorphisms and Post-treatment Apical Periodontitis

Siqueira¹,

Rôças²,

Provenzano³

et al. 2009

Journal of Endodontics

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The Mouthwash: A Non‐Invasive Sampling Method to Study Cytokine Gene Polymorphisms

Abstract: Mouthwash can be the method of choice to study gene polymorphisms in periodontitis and other chronic inflammatory diseases.

Cited by 41 publications

References 22 publications

Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection

Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection

CDKN2BAS is associated with periodontitis in different European populations and is activated by bacterial infection

Relationship Between Fcγ Receptor and Interleukin-1 Gene Polymorphisms and Post-treatment Apical Periodontitis

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