The mRNA Expression Pattern of Skeletal Muscle Regulatory Factors in Divergent Phenotype Swine Breeds

Çınar, Mehmet Ulaş; Fan, Huitao

doi:10.9775/kvfd.2012.6348

Cited by 6 publications

(6 citation statements)

References 26 publications

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“…Principal component analysis applied to gene expression data sets showed similar expression levels of MyoD and myogenin genes in different muscles in different pig breeds such as Duroc and Pietrain. These results differed from those in the current study, possibly because of species differences (avian/mammalian; Cinar and Fan, 2012). Taken together, these findings suggested possible coordinated regulation of MRF gene expression during skeletal muscle development in the embryonic stage.…”

Section: Discussioncontrasting

confidence: 57%

Development of skeletal muscle and expression of myogenic regulatory factors during embryonic development in Jinding ducks (Anas platyrhynchos domestica)

Zhu

Zhang

et al. 2014

Poultry Science

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Section: Discussioncontrasting

confidence: 57%

Development of skeletal muscle and expression of myogenic regulatory factors during embryonic development in Jinding ducks (Anas platyrhynchos domestica)

Zhu

Zhang

et al. 2014

Poultry Science

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“…Thus, different expression patterns could be due to the close proximity of the Myf6 and Myf5 genes on Sus scrofa chromosome 5. However, these findings were different from our results, which may be explained by the different species (avian/mammalian) (Cinar & Fan 2012). The expression levels of Myf5 were less than that of Myf6 in both BM and LM and showed different expression pattern in different muscle, and there was also a different pattern with Myf6 gene.…”

Section: Discussioncontrasting

confidence: 99%

“…PCA results have shown that MyoD gene expression in different muscle tissues was similar in Duroc and Pietrain pig breeds. However, these findings were different from our results, which may be explained by the different species (avian/mammalian) (Cinar & Fan 2012). Taken together, these findings suggested possible coordinated regulation of MyoD and Myf6 gene expression during skeletal muscle development.…”

Section: Discussioncontrasting

confidence: 99%

MyoD and Myf6 gene expression patterns in skeletal muscle during embryonic and posthatch development in the domestic duck (Anas platyrhynchos domestica)

Zhu

Zhang

et al. 2013

J Animal Breeding Genetics

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The MyoD and Myf6 genes, which are muscle regulatory factors (MRFs), play major roles in muscle growth and development and initiate muscle fibre formation via the regulation of muscle-specific gene translation. Therefore, MyoD and Myf6 are potential candidate genes for meat production traits in animals and poultry. The objective of this study was to evaluate MyoD and Myf6 gene expression patterns in the skeletal muscle during early developmental stage of ducks. Gene expression levels were detected using the quantitative RT-PCR method in the breast muscle (BM) and leg muscle (LM) at embryonic days 13, 17, 21, 25, 27, as well as at 1 week posthatching in Gaoyou and Jinding ducks (Anas platyrhynchos domestica). The MyoD and Myf6 gene profiles in the two duck breeds were consistent during early development, and MyoD gene expression showed a 'wave' trend in BM and an approximate 'anti-√' trend in LM. Myf6 gene expression in BM showed the highest level at embryonic day 21, which subsequently decreased, although remained relatively high, while levels at embryonic days 13, 17 and 21 were higher in LM. The results of correlation analysis showed that MyoD and Myf6 gene expression levels were more strongly correlated in LM than in BM in both duck breeds. These results indicated that different expression patterns of the MyoD and Myf6 genes in BM and LM may be related to muscle development and differentiation, suggesting that MyoD and Myf6 are integral to skeletal muscle development.

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“…Taken together, we propose that there might exist certain differences in myogenesis among the three muscles. Furthermore, the myogenesis marker genes ( Myf4 , MyoD , BMP4 , Myf5 , and SRF ) [31,32,33,34], were significantly more highly expressed in PL, which confirmed that PL had higher myogenesis capacity (Figure 2g). The activation of myogenic progenitors (e.g., satellite cells) contributed to the myogenensis of adult skeletal muscle tissues [35], thus we propose that the PL may contain more active myogenic progenitors relative to PL and LDM.…”

Section: Resultsmentioning

confidence: 72%

The miRNA Transcriptome Directly Reflects the Physiological and Biochemical Differences between Red, White, and Intermediate Muscle Fiber Types

Wang

et al. 2015

IJMS

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MicroRNAs (miRNAs) are small non-coding RNAs that can regulate their target genes at the post-transcriptional level. Skeletal muscle comprises different fiber types that can be broadly classified as red, intermediate, and white. Recently, a set of miRNAs was found expressed in a fiber type-specific manner in red and white fiber types. However, an in-depth analysis of the miRNA transcriptome differences between all three fiber types has not been undertaken. Herein, we collected 15 porcine skeletal muscles from different anatomical locations, which were then clearly divided into red, white, and intermediate fiber type based on the ratios of myosin heavy chain isoforms. We further illustrated that three muscles, which typically represented each muscle fiber type (i.e., red: peroneal longus (PL), intermediate: psoas major muscle (PMM), white: longissimus dorsi muscle (LDM)), have distinct metabolic patterns of mitochondrial and glycolytic enzyme levels. Furthermore, we constructed small RNA libraries for PL, PMM, and LDM using a deep sequencing approach. Results showed that the differentially expressed miRNAs were mainly enriched in PL and played a vital role in myogenesis and energy metabolism. Overall, this comprehensive analysis will contribute to a better understanding of the miRNA regulatory mechanism that achieves the phenotypic diversity of skeletal muscles.

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The mRNA Expression Pattern of Skeletal Muscle Regulatory Factors in Divergent Phenotype Swine Breeds

Cited by 6 publications

References 26 publications

Development of skeletal muscle and expression of myogenic regulatory factors during embryonic development in Jinding ducks (Anas platyrhynchos domestica)

Development of skeletal muscle and expression of myogenic regulatory factors during embryonic development in Jinding ducks (Anas platyrhynchos domestica)

MyoD and Myf6 gene expression patterns in skeletal muscle during embryonic and posthatch development in the domestic duck (Anas platyrhynchos domestica)

The miRNA Transcriptome Directly Reflects the Physiological and Biochemical Differences between Red, White, and Intermediate Muscle Fiber Types

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