The Multivariate Regression Statistics Strategy to Investigate Content-Effect Correlation of Multiple Components in Traditional Chinese Medicine Based on a Partial Least Squares Method

Abstract

Amultivariate regression statisticstrategy was developed to clarify multi-components content-effect correlation ofpanaxginseng saponins extract and predict the pharmacological effect by components content. In example 1, firstly, we compared pharmacological effects between panax ginseng saponins extract and individual saponin combinations. Secondly, we examined the anti-platelet aggregation effect in seven different saponin combinations of ginsenoside Rb1, Rg1, Rh, Rd, Ra3 and notoginsenoside R1. Finally, the correlation between anti-platelet aggregation and the content of multiple components was analyzed by a partial least squares algorithm. In example 2, firstly, 18 common peaks were identified in ten different batches of panax ginseng saponins extracts from different origins. Then, we investigated the anti-myocardial ischemia reperfusion injury effects of the ten different panax ginseng saponins extracts. Finally, the correlation between the fingerprints and the cardioprotective effects was analyzed by a partial least squares algorithm. Both in example 1 and 2, the relationship between the components content and pharmacological effect was modeled well by the partial least squares regression equations. Importantly, the predicted effect curve was close to the observed data of dot marked on the partial least squares regression model. This study has given evidences that themulti-component content is a promising information for predicting the pharmacological effects of traditional Chinese medicine.

Ginsenoside Rb1 attenuates intestinal ischemia/reperfusion‑induced inflammation and oxidative stress via activation of the PI3K/Akt/Nrf2 signaling pathway

“…Meyer (Araliaceae family), a traditional herbal medicine that is widely used in Asian countries (5). GRb1 has been reported to protect various organs from IIR injury due to its antioxidant and antiapoptotic effects (6,7). GRb1 increases phosphorylated (p)-protein kinase B (Akt) levels and promotes p-extracellular signal-regulated kinase 1/2-mediated signaling to suppress amyloid β (Aβ)-induced apoptosis (5), whereas exposure to Aβ leads to the accumulation of reactive oxygen species (ROS) and lipid peroxidation.…”

Saponins as Modulators of the Blood Coagulation System and Perspectives Regarding Their Use in the Prevention of Venous Thromboembolic Incidents

“…Table 1 summarizes the results of recent in vitro and in vivo studies, based on various models, on the antiplatelet and antithrombotic activity of individual saponins. For example, some saponins have an effect on the coagulation cascade, including prothrombin and other coagulation factors [18,23]. Some studies report the pharmacological properties of single and specific saponins with antithrombotic, anti-platelet aggregation and anticoagulation effects [20][21][22][23].…”

“…For example, some saponins have an effect on the coagulation cascade, including prothrombin and other coagulation factors [18,23]. Some studies report the pharmacological properties of single and specific saponins with antithrombotic, anti-platelet aggregation and anticoagulation effects [20][21][22][23]. Table 1 summarizes the results of recent in vitro and in vivo studies, based on various models, on the antiplatelet and antithrombotic activity of individual saponins.…”

Application of Proteomics and Metabonomics to Reveal the Molecular Basis of Atractylodis Macrocephalae Rhizome for Ameliorating Hypothyroidism Instead of Hyperthyroidism

“…Chinese herbs have multi-component, multi-path, and multi-target characteristics that can be used to holistically treat diseases (Pan et al, 2020;Wang et al, 2012). One of the major challenges facing the modernization of TCM is elucidating the molecular basis of medicines and the mechanisms underlying the efficacy of medicines used in TCM (Peng et al, 2018;Zhang et al, 2018). New methods are thus essential for exploring the efficacy of these medicines as well as characterizing their molecular basis.…”