The need for individual selection of probiotics containing lactobacilli and enterococci to increase the effectiveness of therapy for campylobacteriosis

Ермоленко, К. Д.; Boldyreva, N.P. Boldyreva; Martens, E. A.; Железова, Л И; Сидоренко, С. В.; Suvorov, A. N.; Ermolenko, E. I.

doi:10.31146/1682-8658-ecg-186-2-88-93

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…In vitro, co-cultivation showed a mutual inhibitory effect between Candida albicans and Lactobacillus plantarum. The authors of the study explain this by competition for nutrients and colonization of the epithelial mucosa of the oral cavity and vagina [12,13]. Also, the co-cultivation of the production strain of lactobacilli and Candida on a solid nutrient medium revealed a significant decrease in the number of colonies for all Candida albicans strains taken in the experiment compared to the control [14].…”

Section: Introductionmentioning

confidence: 99%

Viability and Antagonism of Cryopreserved Lactic Acid Bacteria

K. Kh.,

Z. S.,

A. A.

et al. 2023

KAZATUVC

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Preserving and advancing bioresources involving industrial microorganisms is of paramount importance for every nation. However, long-term storage of these strains often leads to diminished viability and biological activity. Thus, it is crucial to investigate the properties of cryopreserved strains stored at -80°C in a 10% glycerol solution within low-temperature refrigerators. This study aimed to comparatively analyze the viability of 129 lactic acid bacteria strains, including Lactobacillus sp., Lactococcus sp., and Pediococcus sp., cryopreserved from 2006 to 2020. Among them, 93 Lactobacillus sp. strains were categorized into three groups based on storage dates (2006-2007, 2013-2014, and 2017-2020). Viability titers were determined using the standard serial dilution method, counting microorganisms in CFU/ml. Regardless of the storage duration or species affiliation, the study identified lactic acid bacteria strains exhibiting both high (107 - 109 CFU/ml) and low (104-106 CFU/ml) viability titers. Additionally, the antagonistic activity of 33 Lactobacillus sp. strains was investigated using the delayed antagonism method, subdividing them into 17 strains with sufficiently high viability titers and 16 strains with low viability titers. The results revealed that 20% of strains with high viability titers and 27% with low viability titers exhibited relatively high antagonistic activity (with a zone of inhibition ranging from 10 to 18 mm). In both groups, strains with low antagonistic activity (with a zone of inhibition measuring 5-9 mm), particularly against Gram-positive and Gram-negative bacterial test-cultures, predominated. Significantly, 51% of lactobacilli strains demonstrated pronounced antagonism against Candida albicans ATCC- 885-653 test-culture. These findings underscore the practical importance of the study, emphasizing the necessity to analyze and select optimal concentrations of intracellular or extracellular cryopreservatives and to determine the initial viabilitytiter when storing strains for cryopreservation. Tailoring cryopreservation solutions to each strain can enhance the preservation of their original properties, ultimately improving overall preservation quality.

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Section: Introductionmentioning

confidence: 99%

Viability and Antagonism of Cryopreserved Lactic Acid Bacteria

K. Kh.,

Z. S.,

A. A.

et al. 2023

KAZATUVC

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Sensitivity of pathogenic and opportunistic microorganisms to probiotic strains in the light of personalized probiotic therapy

Суранова

2023

Epidemiology and Infectious Diseases

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The purpose of the study. To study the sensitivity of clinical strains of pathogenic and opportunistic bacteria and fungi to microorganisms that are part of probiotic preparations. Materials and methods. The sensitivity of 93 cultures of pathogenic and opportunistic microorganisms (UPM) isolated from the feces of patients with intestinal dysbiosis to strains of probiotic bacteria isolated from commercial probiotic preparations was studied: L. plantarum 8RA-3, E. coli M-17, B. subtilis strain VKPM B-10641, E. faecium SF-68, S. boulardii CNCM I-745 by delayed antagonism method. Results. It was found that pathogenic microorganisms showed low and moderate sensitivity to the probiotic strain of L. plantarum. Sensitivity to the probiotic strain of B. subtilis was detected in S. aureus strains, low or completely absent in P. aeruginosa, Klebsiella spp., S. marcescens. The most sensitive to the probiotic strain were clinical strains of E. coli. Conclusion. UPM and pathogenic microorganisms isolated from the gastrointestinal tract of patients with dysbiosis have different sensitivity to probiotic strains: from high to several probiotics to its complete absence. The empirical approach in prescribing a probiotic does not guarantee a therapeutic effect, which makes it expedient to personify the selection of a probiotic based on an assessment of its antimicrobial activity against isolated pathogens.

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Marketing assessment of the potential of the Russian pharmaceutical market in the framework of etiotropic therapy of children with campylobacteriosis

et al. 2022

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The paper analyzes approaches to etiotropic therapy and structural assessment of the range of drugs for the treatment of campylobacteriosis in children. The methods of content analysis, grouping, data aggregation, comparative and marketing analysis were used. The information base of the study was the State Register of Medicines, federal clinical guidelines for the provision of medical care to children with campylobacteriosis, European and American clinical guidelines, as well as instructions for the medical use of drugs.Comparative analysis showed that Russian clinical guidelines contain 15 international non-proprietary names used for the etiotropic therapy of campylobacteriosis. The analysis carried out by drug producing countries shows that the leading positions in the supply structure are occupied by Russian producers (68.26%). Structuring by dosage forms makes it possible to single out tablet positions (47.46%). At the same time, 36.47% of the range of drugs are approved for use from the age of 12, which limits the implementation of modern approaches to the rational etiotropic therapy of campylobacteriosis in pediatrics. Structural analysis of the range of pharmaceutical substances identifies a significant contribution to the structure of imports of Chinese producers (46.30%), which, in turn, justifies the prospects for import substitution. Solving the problem of improving the quality of medical care for children with campylobacteriosis determines the prospects for further research based on the principles of evidence-based medicine using the tools of mathematical-statistical and pharmacoeconomic analyses.

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The need for individual selection of probiotics containing lactobacilli and enterococci to increase the effectiveness of therapy for campylobacteriosis

Cited by 3 publications

References 19 publications

Viability and Antagonism of Cryopreserved Lactic Acid Bacteria

Viability and Antagonism of Cryopreserved Lactic Acid Bacteria

Sensitivity of pathogenic and opportunistic microorganisms to probiotic strains in the light of personalized probiotic therapy

Marketing assessment of the potential of the Russian pharmaceutical market in the framework of etiotropic therapy of children with campylobacteriosis

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