The next-generation sequencing technology and application

Abstract

As one of the key technologies in biomedical research, DNA sequencing has not only improved its productivity with an exponential growth rate but also been applied to new areas of application over the past few years. This is largely due to the advent of newer generations of sequencing platforms, offering ever-faster and cheaper ways to analyze sequences. In our previous review, we looked into technical characteristics of the nextgeneration sequencers and provided prospective insights into their future development. In this article, we present a brief overview of the advantages and shortcomings of key commercially available platforms with a focus on their suitability for a broad range of applications.

Molecular Genetics and Genomics of Hepatocellular Carcinoma

“…Today, the state of DNA sequencing technologies is in a greater flux than ever before. With this foreseeable evolution, comes new possibilities not only in the field of large-scale genomic sciences from medicine to agriculture and plant sciences coupled with new challenges in data storage and analysis, but also for practical use such as clinical utilization for routine diagnostics [74][75][76][77][78]. Currently, several methods are already established, made significant impact on the field of genomics by having reputable track record for many different published applications and some are in the process of building confidence, some are yet to be tested and perfected [78][79][80][81][82][83][84].…”

Detecção e análise filogenética de vírus em melancia e construção de novos vetores virais para expressão de proteínas em células de inseto

“…Esta técnica utiliza terminadores reversíveis em um processo cíclico que compreende a incorporação de nucleotídeos, imagens de fluorescência e clivagem do radical terminador (todo esse processo ocorre dentro de uma câmara) (Metzker, 2010;Zhou et al, 2010;Barba et al, 2014).…”

“…A tecnologia da plataforma de RNA-seq Illumina é uma ferramenta que pode ser usada para facilitar a identificação, caracterização e classificação taxonômica de vírus. Permite fazer a análise da diversidade e variabilidade de vírus com detecção de partes de sequências ou mesmo genomas inteiros Ng et al, 2011b;Zhou et al, 2010).…”

“…A tecnologia do NGS revolucionou o processo de sequenciamento genômico com a geração de bilhões de sequências de uma vez de forma rápida e a baixo custo (Metzker, 2010;Zhou et al, 2010;Barba et al, 2014). O número de sequências disponíveis nos bancos de dados genômicos vem crescendo exponencialmente com uso de modernas plataformas de sequenciamento desenvolvidas no final da década de 1990 e comercializadas por volta de…”

An island-based approach for RNA-SEQ differential expression analysis.

“…This process continues for a number of cycles and the light for each incorporation is recorded for each bead [30]. Thus, the intensity of the light recorded for a particular well indicates the number of incorporated nucleotides [169]. Initially, the Roche 454 Sequencer had a read length of 100-150 bp, but the more recent, Roche GS FLX Titanium XL+ can produce an average read length up to 1000 bp.…”