The Nitric Oxide Production in the Moss Physcomitrella patens Is Mediated by Nitrate Reductase

Medina-Andrés, Rigoberto; Peralta, Alejandro; Saucedo-Vázquez, Juan Pablo; Napsucialy-Mendivil, Selene; Pimentel, Jaime A.; Sosa‐Torres, Martha E.; Dubrovsky, Joseph G.; Lira-Ruan, Verónica

doi:10.1371/journal.pone.0119400

Cited by 31 publications

(16 citation statements)

References 69 publications

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“…Biofilm formation is often critical to host–symbiont communication, especially when it is mediated by diffusible signaling molecules ( Norsworthy and Visick, 2013 ), so biofilm formation may favor host–symbiont NO-mediated communication and enhance currency exchange. NO-mediated signaling may also allow the host to guide cyanobacteria migration and colonization of new plant tissue as NO is highly produced in protonema cells during the growth of the moss Physcomitrella patens ( Medina-Andres et al , 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Feathermoss and epiphytic Nostoc cooperate differently: expanding the spectrum of plant–cyanobacteria symbiosis

et al. 2017

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Dinitrogen (N2)-fixation by cyanobacteria in symbiosis with feathermosses is the primary pathway of biological nitrogen (N) input into boreal forests. Despite its significance, little is known about the cyanobacterial gene repertoire and regulatory rewiring needed for the establishment and maintenance of the symbiosis. To determine gene acquisitions and regulatory changes allowing cyanobacteria to form and maintain this symbiosis, we compared genomically closely related symbiotic-competent and -incompetent Nostoc strains using a proteogenomics approach and an experimental set up allowing for controlled chemical and physical contact between partners. Thirty-two gene families were found only in the genomes of symbiotic strains, including some never before associated with cyanobacterial symbiosis. We identified conserved orthologs that were differentially expressed in symbiotic strains, including protein families involved in chemotaxis and motility, NO regulation, sulfate/phosphate transport, and glycosyl-modifying and oxidative stress-mediating exoenzymes. The physical moss–cyanobacteria epiphytic symbiosis is distinct from other cyanobacteria–plant symbioses, with Nostoc retaining motility, and lacking modulation of N2-fixation, photosynthesis, GS-GOGAT cycle and heterocyst formation. The results expand our knowledge base of plant–cyanobacterial symbioses, provide a model of information and material exchange in this ecologically significant symbiosis, and suggest new currencies, namely nitric oxide and aliphatic sulfonates, may be involved in establishing and maintaining the cyanobacteria–feathermoss symbiosis.

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Section: Discussionmentioning

confidence: 99%

Feathermoss and epiphytic Nostoc cooperate differently: expanding the spectrum of plant–cyanobacteria symbiosis

et al. 2017

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“…In addition, our results explain the described effect of tungstate to inhibit NO in plant (Xiong et al ). Tungstate will certainly inhibit NR activity, without affecting its diaphorase activity, but it will affect ARC and all other molybdoenzymes (Medina‐Andres et al ). We show herein that functionality of the whole NR was not necessary for NO production with ARC, provided that the diaphorase activity of NR is active (mutant 301; Fig.…”

Section: Discussionmentioning

confidence: 99%

A dual system formed by the ARC and NR molybdoenzymes mediates nitrite‐dependent NO production in Chlamydomonas

Chamizo-Ampudia

Sanz-Luque

Llamas

et al. 2016

Plant Cell & Environment

137

120

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Nitric oxide (NO) is a relevant signal molecule involved in many plant processes. However, the mechanisms and proteins responsible for its synthesis are scarcely known. In most photosynthetic organisms NO synthases have not been identified, and Nitrate Reductase (NR) has been proposed as the main enzymatic NO source, a process that in vitro is also catalysed by other molybdoenzymes. By studying transcriptional regulation, enzyme approaches, activity assays with in vitro purified proteins and in vivo and in vitro NO determinations, we have addressed the role of NR and Amidoxime Reducing Component (ARC) in the NO synthesis process. N\R and ARC were intimately related both at transcriptional and activity level. Thus, arc mutants showed high NIA1 (NR gene) expression and NR activity. Conversely, mutants without active NR displayed an increased ARC expression in nitrite medium. Our results with nia1 and arc mutants and with purified enzymes support that ARC catalyses the NO production from nitrite taking electrons from NR and not from Cytb5-1/Cytb5-Reductase, the component partners previously described for ARC (proposed as NOFNiR, Nitric Oxide-Forming Nitrite Reductase). This NR-ARC dual system would be able to produce NO in the presence of nitrate, condition under which NR is unable to do it.

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“…Reduction of nitrate via nitrate reductase also leads to production of nitric oxide (NO) ( 63 , 64 ), signaling reactive oxygen species (ROS) active in diverse species ( 65 , 66 ), including algae ( 61 ), that is known to heighten the cellular defense responses of cells to stress ( 66 – 68 ). It is required for resistance to viruses of Arabidopsis ( 69 ) and rice ( 70 ), and ROS are also known to modulate the response of E. huxleyi to viruses ( 45 ).…”

Section: Discussionmentioning

confidence: 99%

Prasinovirus Attack of Ostreococcus Is Furtive by Day but Savage by Night

Derelle

Yau

Moreau

et al. 2018

J Virol

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Prasinoviruses are large DNA viruses that infect diverse genera of green microalgae worldwide in aquatic ecosystems, but molecular knowledge of their life cycles is lacking. Several complete genomes of both these viruses and their marine algal hosts are now available and have been used to show the pervasive presence of these species in microbial metagenomes. We have analyzed the life cycle of Ostreococcus tauri virus 5 (OtV5), a lytic virus, using transcriptome sequencing (RNASeq) from 12 time points of healthy or infected Ostreococcus tauri cells over a day/ night cycle in culture. In the day, viral gene transcription remained low while host nitrogen metabolism gene transcription was initially strongly repressed for two successive time points before being induced for 8 h, but during the night, viral transcription increased steeply while host nitrogen metabolism genes were repressed and many host functions that are normally reduced in the dark appeared to be compensated either by genes expressed from the virus or by increased expression of a subset of 4.4% of the host's genes. Some host cells underwent lysis progressively during the night, but a larger proportion were lysed the following morning. Our data suggest that the life cycles of algal viruses mirror the diurnal rhythms of their hosts.IMPORTANCE Prasinoviruses are common in marine environments, and although several complete genomes of these viruses and their hosts have been characterized, little is known about their life cycles. Here we analyze in detail the transcriptional changes occurring over a 27-h-long experiment in a natural diurnal rhythm, in which the growth of host cells is to some extent synchronized, so that host DNA replication occurs late in the day or early in the night and cell division occurs during the night. Surprisingly, viral transcription remains quiescent over the daytime, when the most energy (from light) is available, but during the night viral transcription activates, accompanied by expression of a few host genes that are probably required by the virus. Although our experiment was accomplished in the lab, cyclical changes have been documented in host transcription in the ocean. Our observations may thus be relevant for eukaryotic phytoplankton in natural environments.

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The Nitric Oxide Production in the Moss Physcomitrella patens Is Mediated by Nitrate Reductase

Cited by 31 publications

References 69 publications

Feathermoss and epiphytic Nostoc cooperate differently: expanding the spectrum of plant–cyanobacteria symbiosis

Feathermoss and epiphytic Nostoc cooperate differently: expanding the spectrum of plant–cyanobacteria symbiosis

A dual system formed by the ARC and NR molybdoenzymes mediates nitrite‐dependent NO production in Chlamydomonas

Prasinovirus Attack of Ostreococcus Is Furtive by Day but Savage by Night

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