2014
DOI: 10.1371/journal.pone.0084891
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The Non-JAZ TIFY Protein TIFY8 from Arabidopsis thaliana Is a Transcriptional Repressor

Abstract: Jasmonate (JA) signalling is mediated by the JASMONATE-ZIM DOMAIN (JAZ) repressor proteins, which are degraded upon JA perception to release downstream responses. The ZIM protein domain is characteristic of the larger TIFY protein family. It is currently unknown if the atypical member TIFY8 is involved in JA signalling. Here we show that the TIFY8 ZIM domain is functional and mediated interaction with PEAPOD proteins and NINJA. TIFY8 interacted with TOPLESS through NINJA and accordingly acted as a transcriptio… Show more

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Cited by 56 publications
(41 citation statements)
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“…The ZIM domain of JAZ proteins mediates their homo- or heterodimerization ( Chini et al , 2009 ; Chung and Howe, 2009 ; Chung et al , 2009 ), but JAZ7 appears to be the only JAZ protein incapable of homodimerizing or forming heterodimers with other JAZ proteins ( Chini et al , 2009 ; Chung and Howe, 2009 ; reviewed by Pauwels and Goossens, 2011 ). Another TIFY-containing protein not capable of interacting with JAZ proteins is the non-JAZ protein TIFY8 ( Cuéllar Pérez et al , 2014 ). Although TIFY8 has a functional ZIM domain that mediates transcriptional repression by recruiting TPL via NINJA, its ZIM domain does not confer interactions with JAZ proteins.…”
Section: Discussionmentioning
confidence: 99%
“…The ZIM domain of JAZ proteins mediates their homo- or heterodimerization ( Chini et al , 2009 ; Chung and Howe, 2009 ; Chung et al , 2009 ), but JAZ7 appears to be the only JAZ protein incapable of homodimerizing or forming heterodimers with other JAZ proteins ( Chini et al , 2009 ; Chung and Howe, 2009 ; reviewed by Pauwels and Goossens, 2011 ). Another TIFY-containing protein not capable of interacting with JAZ proteins is the non-JAZ protein TIFY8 ( Cuéllar Pérez et al , 2014 ). Although TIFY8 has a functional ZIM domain that mediates transcriptional repression by recruiting TPL via NINJA, its ZIM domain does not confer interactions with JAZ proteins.…”
Section: Discussionmentioning
confidence: 99%
“…For the construction of pART7RFP‐virE3 (pSDM4508), the RFP ORF was amplified by PCR with the primer set 5′‐GGGGTACCATGGCCTCCTCCGAGGACGTC‐3′ and 5′‐TATGGATCCGGCGCCGGTGGAGTGGCGGCC‐3′ on pART7‐RFP (pSDM4336) and cloned into pJET1.2, then the RFP ORF was excised from pJET1,2‐RFP with Kpn I/ Bam HI and cloned into pART7YFP‐virE3 digested with Kpn I/ Bam HI to generate pART7RFP‐virE3. The TIFY8 protein was used as a nuclear marker (Cuéllar Pérez et al ., ); to this end the TIFY8 ORF was amplified by PCR with the primer set 5′‐GCGTCGACAATGATGGTGAACCACAACAATG‐3′ and 5′‐GCGTCGACTGTGGCTTCTTTTTCAGGATC‐3′ and cloned into pTH2 digested with Sal I to generate a construct encoding the TFY8–GFP fusion protein (pSDM4509). The plasmid with the plastid‐mCherry (CD3‐1000) marker was previously described (Nelson et al ., ).…”
Section: Methodsmentioning
confidence: 99%
“…The JAZ proteins belong to the TIFY protein family, which, besides 12 JAZ proteins, also comprises TIFY8 and the two PEAPOD (PPD) proteins in Arabidopsis, all of which can interact with the Arabidopsis NINJA (Pauwels et al ., ; Pauwels and Goossens, ; Cuéllar Pérez et al ., ). To assess whether MtNINJA displayed similar protein affinities, we first mined the M. truncatula genome for homologues of JAZ, PPD and TIFY8.…”
Section: Resultsmentioning
confidence: 97%