The novel ethylene-responsive factor CsERF025 affects the development of fruit bending in cucumber

Wang, Chunhua; Xin, Ming; Zhou, Xiuyan; Liu, Chunhong; Li, Shengnan; Liu, Dong; Xu, Yuan; Qin, Zhiwei

doi:10.1007/s11103-017-0671-z

Cited by 17 publications

(10 citation statements)

References 41 publications

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“…The ethylene signal transduction-related genes were expressed predominantly in the fruits, followed by the genes coding for auxin production ( Supplementary Table S5). In a previous report, ethylene was found to promote cucumber fruit curving 21 . Further functional pathway enrichment analysis showed that tryptophan metabolism, ribosomes, phenylpropanoid biosynthesis, and six other pathways had significant enrichment (Fig.…”

Section: Transcriptome Analysis Of Curved Cucumber Fruitsmentioning

confidence: 83%

“…A study by Wang et al 21 showed that 2 DPA was a key time point to determine whether cucumber fruits would become curved or straight. A transcriptome expression profile analysis of curved (including the concave and convex sides) and straight fruits at 2 DPA showed that upregulated genes on the convex side were important determinants of cucumber fruit curvature 21 . Consequently, in this study, we focused on the difference between the concave (C1) and convex (C2) sides of the curved fruits at 2 DPA.…”

Section: Library Construction and Transcriptome Analysismentioning

confidence: 99%

“…Information in the Sequence Read Archive database (accession number: SRP111902) was used for transcriptome analysis 21 . After trimming the adaptor sequences, we obtained clean raw reads from the raw sequence reads, which were filtered based on base quality (Q ≥ 20) 22 .…”

Section: Library Construction and Transcriptome Analysismentioning

confidence: 99%

“…S4). This approach did not achieve the desired effect because both ethylene and auxin synthesis were inhibited by AVG, and ethylene has been reported to contribute to cucumber fruit curving 21 . Overall, asymmetric auxin distribution led to uneven growth of both sides of the fruit, resulting in the formation of curved fruits.…”

Section: Auxin Distribution Directly Controls Fruit Curvingmentioning

confidence: 99%

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The curvature of cucumber fruits is associated with spatial variation in auxin accumulation and expression of a YUCCA biosynthesis gene

Wang

Zhou

et al. 2020

Hortic Res

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Fruit curving lowers the commercial value of cucumber and leads to significant economic losses. The mechanism driving the abnormal curving of cucumber is largely unknown. Through our previous work, we discovered that 2 days post-anthesis (DPA) was the key time point at which various phenotypic and genotypic characteristics of cucumber fruits are determined. Here, we analyzed the transcriptome of the concave (C1) and convex (C2) sides of curved fruits at 2 DPA by Gene Ontology (GO) enrichment and functional pathway enrichment analyses and identified auxin as a putative factor influencing fruit curvature. Changes in the curve angle in the fruits and exogenous auxin treatment analyses showed that asymmetric auxin distribution induces fruit curving. Identification of differentially expressed genes (DEGs) related to auxin and qPCR validation showed that CsYUC10b had the most significant differential expression when both sides of the curved fruits were compared. Gene functional analysis showed that the transcript levels of CsYUC10b and the auxin concentration were even on both sides of the fruit in CsYUC10b-overexpressing plants, which in turn contributed to an equal rate of growth of both sides of cucumber fruits and resulted in a straight shape of the fruits. Thus, we conclude that CsYUC10b promotes the formation of straight cucumber fruits, with possible applications in the production and breeding of cucumber.

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Section: Transcriptome Analysis Of Curved Cucumber Fruitsmentioning

confidence: 83%

Section: Library Construction and Transcriptome Analysismentioning

confidence: 99%

Section: Library Construction and Transcriptome Analysismentioning

confidence: 99%

Section: Auxin Distribution Directly Controls Fruit Curvingmentioning

confidence: 99%

See 2 more Smart Citations

The curvature of cucumber fruits is associated with spatial variation in auxin accumulation and expression of a YUCCA biosynthesis gene

Wang

Zhou

et al. 2020

Hortic Res

Self Cite

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show abstract

“…One of the ethylene receptor genes, CsERF025, was identified in a study devoted to fruit bending in cucumber. Overexpression of this gene was able to increase ethylene production and upregulate the expressions of the ethylene synthesis-related genes ACS and ACO (Wang et al 2017), which could also influence flower sex.…”

Section: Ethylene Receptors and The Ethylene Signaling Cascade In Cucmentioning

confidence: 99%