The Novel Fusion Proteins, GnRH-p53 and GnRHIII-p53, Expression and Their Anti-Tumor Effect

Abstract

p53, one of the most well studied tumor suppressor factor, is responsible to a variety of damage owing to the induction of apoptosis and cell cycle arrest in the tumor cells. More than 50% of human tumors contain mutation or deletion of p53. Gonadotrophin-releasing hormone (GnRH), as the ligand of Gonadotrophin-releasing hormone receptor (GnRH-R), was used to deliver p53 into tumor cells. The p53 fusion proteins GnRH-p53 and GnRH iii-p53 were expressed and their targeted anti-tumor effects were determined. GnRH mediates its fusion proteins transformation into cancer cells. The intracellular delivery of p53 fusion proteins exerted the inhibition of the growth of H1299 cells in vitro and the reduction of tumor volume in vivo. Their anti-tumor effect was functioned by the apoptosis and cell cycle arrest induced by p53. Hence, the fusion protein could be a novel protein drug for anti-tumor therapy.

Design, production and purification of a novel recombinant gonadotropin-releasing hormone associated peptide as a spawning inducing agent for fish

“…Xu et al (45) reported that chimeric peptide (recombinant GnRH) was expressed in an inclusion body and the target peptide purified in denaturing condition (45). Two fusion proteins, GnRH-P53 and GnRHΙΙΙ-p53 were expressed intracellularly and dissolved in 8M urea and the purification process was performed in denaturing condition with Ni-NAT affinity chromatography (48).…”

Control of Protein Activity and Gene Expression by Cyclofen‐OH Uncaging

“…Introduction of appropriate steroids releases the fused protein from its chaperone complex and activates its function. That approach was used to induce the activity of ag reat variety of proteins: transcription factors [26][27][28][29] (such as Gal4, GATA,o rc -Jun), recombinases [30][31] (such as Flp or Cre), kinases [32] (such as Erb1 or Src), oncogenes [33][34] (such as B-Raf or cMyc), tumor suppressors [35][36] (such as p53), and enzymes [37] (such as b-galactosidase). The caging ands ubsequentp hotoreleaseo fs teroid hor-monest hus offers av ersatile, generic, and proven approacht o quick photocontrol of the activity of many proteins at acellular level.…”