The nuclear elongation factor-1α gene: a promising marker for phylogenetic studies of Triatominae (Hemiptera: Reduviidae)

Díaz, Sebastián Pérez; Triana-Chávez, Omar; Gómez-Palacio, Andrés

doi:10.1016/j.meegid.2016.06.010

Cited by 7 publications

(3 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genomic DNA was extracted from 3 legs of the triatomines using the DNeasy Blood & Tissue Kit (Qiagen, German), according to the manufacturer’s recommendations. The mitochondrial 16S rRNA and the cytochrome b (CytB) gene were PCR-amplified using the primers 16S–F (5′-CGCCTGTTTATCAAAAACAT-3′), 16S–R (5′-CTCCGGTTTGAACTCAGATCA-3′) CYTB7432 (5′-GGACGWGGWATTTATTATGGATC-3′), and CYTB7433 (5′-GCWCCAATTCARGTTARTAA-3′) [ 15 , 16 ]. The nuclear ribosomal 28S rRNA gene was PCR-amplified using the primers 28S–F (5′-GCGAGTCGTGTTGCTTGATAGTGCAG-3′) and 28S–R (5′-TTGGTCCGTGTTTCAAGACGGG-3′) [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

First records of Triatoma rubrofasciata (De Geer, 1773) (Hemiptera, Reduviidae) in Foshan, Guangdong Province, Southern China

et al. 2017

View full text Add to dashboard Cite

BackgroundTriatomines, also known as kissing bugs, which are found throughout the world and especially in Latin America, are well known natural vectors that transmit American trypanosomiasis, also called Chagas disease. In China, the presence of two species of Triatoma (Triatoma rubrofasciata and T. sinica) was recorded in the past. Due to the growing population and the increasing risk of the global spread of Chagas disease, triatomines became a potential public health nuisance, and in 2016, we started monitoring triatomine activities in southern China.MethodsTriatomine specimens were collected by the National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, and identified by their morphological characteristics under a dissecting microscope. In addition to morphological analysis, the genomic DNA of the specimens was extracted, and the mitochondrial 16S rRNA, the cytochrome b (CytB) gene and the nuclear ribosomal 28S rRNA gene were PCR-amplified to analyze and confirm the species genetically.ResultsOne female adult insect and one male adult insect were collected in a dwelling in the rural area of Shunde County, Foshan City, Guangdong Province, China (22°42′44.63″N, 113°08′45.34″E). The results from the morphological and genetic analyses indicated that these triatomines were T. rubrofasciata.ConclusionsThis is the first time that the occurrence of T. rubrofasciata has been confirmed in Foshan City, Guangdong Province in southern China. Further studies are needed to reach a clearer understanding of the ecology of this species of triatomine, since it has been found to be naturally infected by Trypanosoma cruzi and T. conorhini and there is evidence of its domiciliation capabilities.Electronic supplementary materialThe online version of this article (doi:10.1186/s40249-017-0342-y) contains supplementary material, which is available to authorized users.

show abstract

Section: Methodsmentioning

confidence: 99%

First records of Triatoma rubrofasciata (De Geer, 1773) (Hemiptera, Reduviidae) in Foshan, Guangdong Province, Southern China

et al. 2017

View full text Add to dashboard Cite

show abstract

“…(Figure 5). In relation to the other tribes (mostly the Triatomini tribe), the Rhodniini tribe is of monophyletic origin [114][115][116][117][118][119]. In studies in which specimens of the Cavernicolini and Borbodelini tribes were included, these tribes were grouped with the Rhodniini and not with the Triatomini, although all tribes preserved their monophyletic status.…”

Section: Sequencing Of Mitochondrial and Nuclear Markersmentioning

confidence: 99%

“…Under this hypothesis it is assumed that R. colombiensis and R. pallescens are sister species. Thus, the ancestor of the pictipes lineage was dispersed to the northern part of the then low eastern mountain range of Colombia in the late Miocene (11-6 Ma) [7,131] or the mid-Miocene (16-11 Ma) [116]. Thus, the diversification of the pallescens and pictipes could be explained by a vicariance, either caused by the formation of the Pebas system [130] or by the elevation of the Andes mountain range during the Pliocene (5 Ma) [7,131].…”

Section: Lineages Hypothesismentioning

confidence: 99%

Taxonomy, Evolution, and Biogeography of the Rhodniini Tribe (Hemiptera: Reduviidae)

et al. 2020

View full text Add to dashboard Cite

The Triatominae subfamily includes 151 extant and three fossil species. Several species can transmit the protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease, significantly impacting public health in Latin American countries. The Triatominae can be classified into five tribes, of which the Rhodniini is very important because of its large vector capacity and wide geographical distribution. The Rhodniini tribe comprises 23 (without R. taquarussuensis) species and although several studies have addressed their taxonomy using morphological, morphometric, cytogenetic, and molecular techniques, their evolutionary relationships remain unclear, resulting in inconsistencies at the classification level. Conflicting hypotheses have been proposed regarding the origin, diversification, and identification of these species in Latin America, muddying our understanding of their dispersion and current geographic distribution. Clarifying these factors can help for the design of vector control strategies. The aim of this review is to depict the different approaches used for taxonomy of the Rhodniini and to shed light on their evolution and biogeography.

show abstract

Evolution, Systematics, and Biogeography of the Triatominae, Vectors of Chagas Disease

Monteiro

Weirauch

Felix

et al. 2018

Advances in Parasitology

129

245

View full text Add to dashboard Cite

The nuclear elongation factor-1α gene: a promising marker for phylogenetic studies of Triatominae (Hemiptera: Reduviidae)

Cited by 7 publications

References 51 publications

First records of Triatoma rubrofasciata (De Geer, 1773) (Hemiptera, Reduviidae) in Foshan, Guangdong Province, Southern China

First records of Triatoma rubrofasciata (De Geer, 1773) (Hemiptera, Reduviidae) in Foshan, Guangdong Province, Southern China

Taxonomy, Evolution, and Biogeography of the Rhodniini Tribe (Hemiptera: Reduviidae)

Evolution, Systematics, and Biogeography of the Triatominae, Vectors of Chagas Disease

Contact Info

Product

Resources

About