The nucleotide sequence of a cDNA clone encoding acyl carrier protein (ACP) from<i>Brassica campestris</i>seeds

Rose, Ronald E.; Dejesus, Cataline E.; Moylan, S. L.; Ridge, Neal P.; Scherer, Donna E.; Knauf, Vic

doi:10.1093/nar/15.17.7197

Cited by 37 publications

(18 citation statements)

References 7 publications

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“…As observed with other ACPs, the carboxy termini regions of Arabidopsis ACP-1 and ACP-2 isoforms have higher sequence similarity (79% over the last 28 amino acids) than do the amino termini (54% over the first 28 Fig. 2a and Fig.…”

Section: Structure Of Two Acp Clones From Arabidopsissupporting

confidence: 72%

“…In spinach, a major isoform (ACP-I) is expressed only in leaves, whereas ACP-II, a minor isoform in leaves, is apparently "constitutive" as it is also found in roots and developing seeds (21,31). ACP cDNA clones have been isolated from spinach leaves (30) and roots (31), barley leaves (13), and Brassica campestris (28) and B. napus (29) developing seeds. In addition, ACP genomic clones have been characterized from Arabidopsis (18,25), B. napus (32), and barley (14).…”

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confidence: 99%

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Expression of Constitutive and Tissue-Specific Acyl Carrier Protein Isoforms in Arabidopsis

Hloušek-Radojčić¹,

Post-Beittenmiller²,

Ohlrogge³

1992

Plant Physiol.

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We have characterized the occurrence and expression of multiple acyl carrier protein (ACP) isoforms in Arabidopsis thaliana (L.) Heynh ecotype Columbia. Immunoblot analysis of ACPs from Arabidopsis tissues separated by native polyacrylamide gel electrophoresis and I molar urea polyacrylamide gel electrophoresis revealed a complex pattem of multiple ACP isoforms. All tissues examined (leaves, roots, and seeds) expressed at least three forms of ACP. The immunoblot identifications of ACP bands were confirmed by acylation of ACP extracts with Escherichia coli acyl-ACP synthetase. A full-length cDNA clone has been isolated that has 70% identity with a previously characterized Arabidopsis genomic ACP clone (ACP-1) (MA Post-Beittenmiller, A HlousekRadojcic, JB Ohirogge [1989] Nucleic Acids Res 17: 1777). Based on RNA blot analysis, the cDNA clone represents an ACP that is expressed in leaves, seeds, and roots. In order to identify the protein products of each known ACP gene, their mature coding sequences have been expressed in E. coli. Using polymerase chain reactions, exons 11 and IlIl of the genomic ACP-1 clone and the mature coding sequences of the ACP-2 cDNA clone were subcloned into E. coil expression vectors. Site-directed mutagenesis was used to convert the amino acid sequence of the ACP-2 cDNA clone to that of the A2 clone of Lamppa and Jacks ([1991] Plant Mol Biol 16: 469-474), ACP-3. The three E. coli-expressed proteins have different mobilities on polyacrylamide gel electrophoresis gels and each comigrates with a different Arabidopsis ACP isoform expressed in leaves, seeds, and roots. Thus, all of the three cloned ACPs appear to be constitutively expressed Arabidopsis ACPs. In addition to these three ACP isoforms, protein blots indicate that seed, leaf, and root each express one or more tissue-specific isoforms.

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Section: Structure Of Two Acp Clones From Arabidopsissupporting

confidence: 72%

mentioning

confidence: 99%

Expression of Constitutive and Tissue-Specific Acyl Carrier Protein Isoforms in Arabidopsis

Hloušek-Radojčić¹,

Post-Beittenmiller²,

Ohlrogge³

1992

Plant Physiol.

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“…2. (39), Arabidopsis thaliana (A. t.) (40), and Brassica campestris (B. c.) (41). Symbols are as in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…4. Amino acid sequence identities between AcpA and the others are 56% for E. coli (36), 59o for Anabaena variabilis (37), 44% for spinach ACP-I (38), 36% for spinach ACP-II (39), 43% for Arabidopsis thaliana (40), and 43% for Brassica campestris (41). The serine residue at position 38, which is the attachment site for the prosthetic group phosphopantetheine (42), is conserved among all the acyl carrier proteins compared.…”

Section: Methodsmentioning

confidence: 99%

The plastid genome of Cryptomonas phi encodes an hsp70-like protein, a histone-like protein, and an acyl carrier protein.

Wang

Liu

1991

Proc. Natl. Acad. Sci. U.S.A.

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The plastid genome of Cryptomonas 4), a cryptomonad alga, contains three genes that have not previously been found in any organellar genome. Each of these genes encodes a functional class of organellar gene product not previously reported. The first gene, dnaK, encodes a polypeptide of the hsp7O heat shock protein family. The predicted amino acid sequence of the DnaK protein is-54% identical to that of the Escherichia coli hsp70 protein (DnaK), 50-53% identical to that of two nucleus-encoded mitochondrial hsp70 proteins, and 43-46% identical to that of several eukaryotic cytoplasmic members of the hsp7O protein family. The second gene, hipA, encodes a polypeptide resembling bacterial histonelike proteins. The predicted amino acid sequence of the HlpA protein is 25-53% identical to that of several bacterial histonelike proteins, and the identity increases to 39-76% over a conserved region corresponding to the Iong arm that binds DNA. The third gene, acpA, encodes an acyl carrier protein, which is a key cofactor in the synthesis and metabolism of fatty acids. Its predicted amino acid sequence is 36-59% identical to that ofeubacterial and plant chloroplast (nucleus-encoded) acyl carrier proteins.

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“…The limited presently available evidence suggests that ACP is synthesized as follows (11,16,17,24,28,30,35): Nuclear encoded messages are translated in the cytosol into (apo)-preACP which undergoes two types of processing. That is, the prosthetic group 4'-phosphopantetheine is added to a serine residue.…”

Section: Introductionmentioning

confidence: 99%

Three cDNA clones for barley leaf acyl carrier proteins I and III

Hansen

1987

Carlsberg Res. Commun.

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Three cDNA clones for acyl carrier protein (ACP) isolated from a Lgtl I cDNA library prepared from greening barley seedling leaves using a 66'mer oligonucleotide probe have been sequenced, oACP 2 (371 bases) and pACP 11 (768 bases) code for the 90 amino acid chloroplast located ACP I. pACP I (520 bases) codes for a hitherto unknown protein with 83 amino acids that has been designated ACP Ill. The primary structure of its 27 amino acid partial transit peptide bears no obvious similarities to the transit peptide of ACP I. Northern analysis of immature caryopses' poly A+ RNA using the 66'mer probe reveals the presence of an ACP mRNA of significantly smaller size than the ACP mRNAs detectable in the leaf.

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The nucleotide sequence of a cDNA clone encoding acyl carrier protein (ACP) fromBrassica campestrisseeds

Cited by 37 publications

References 7 publications

Expression of Constitutive and Tissue-Specific Acyl Carrier Protein Isoforms in Arabidopsis

Expression of Constitutive and Tissue-Specific Acyl Carrier Protein Isoforms in Arabidopsis

The plastid genome of Cryptomonas phi encodes an hsp70-like protein, a histone-like protein, and an acyl carrier protein.

Three cDNA clones for barley leaf acyl carrier proteins I and III

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