The nucleotide sequence of the mouse embryonic β-like y-globin messenger RNA as determined from cloned cDNA

v-abl activates embryonic globin gene expression in mouse erythroleukemia cells.

“…4, v-abl-transfected but not -untransfected mouse erythroleukemia cells contained (27)(28)(29)(30)(31). (A) Poly(A)+ mRNA was electrophoresed on a denaturing gel, transferred to DBM paper and hybridized at 420C for 36 hr in 50% (vol/vol) formamide, 6x SSC (1x SSC = 0.15 M NaCl/0.015 M sodium citrate, pH 7.0), 1x Denhardt's, 0.1% NaDodSO4, 0.1 mg of salmon sperm DNA/ml to a 32P-labeled 1.3-kb HincHI fragment of a cloned mouse embryonic "y" cDNA (32), which was a gift of Clyde Hutchison (University of North Carolina, Chapel Hill). The mouse embryonic "y" gene is a "p-like" gene expressed only in mouse embryos (25,32 mouse embryonic "y" globin coding for RNA sequences that protected a mouse embryonic "y" globin cDNA probe in a manner identical to that seen with the reticulocyte RNA of 9-to 10-day-old mouse embryos.…”