The Nutritional Value and Biological Activity of Concentrated Protein Fraction of Potato Juice

Abstract

Potato protein is recognized as one of the most valuable nonanimal proteins due to the high content of essential amino acids. So far, it has not been used in human nutrition on a large scale due to technological limitations regarding its acquisition. In this study, the protein fraction of potato juice was concentrated with the use of membrane separation. The obtained potato juice protein concentrate (PJPC) was characterized in terms of nutritional value and biological activity, and the amino acid composition, mineral content, and antioxidant properties were determined. Moreover, in vitro cytotoxic activity against cancer cells of the gastrointestinal tract was investigated. The results of the present study indicate that PJPC is an excellent source of lysine and threonine, while leucine is its limiting amino acid, with an amino acid score (AAS) of 65%. Moreover, PJPC contains substantial amounts of Fe, Mn, K, and Cu. As demonstrated experimentally, PJPC is also characterized by higher antioxidant potential than potato itself. Biological activity, however, is not limited to antioxidant activity alone. Cytotoxicity studies using a gastric cancer cell line (Hs 746T), a colon cancer cell line (HT-29), and human colon normal cells (CCD 841 CoN) proved that PJPC is characterized by selective activity against cancer cells. It can thus be concluded that the developed method of producing protein concentrate from potato juice affords a product with moderate nutritional value and interesting biological activity.

Comparison of Lupinus angustifolius protein digestibility in dependence on protein, amino acids, trypsin inhibitors and polyphenolic compounds content

“…The digestibility of the received preparations was determined in vitro with the method simulating multi‐enzymatic two‐stage (gastric and intestinal) digestion (Butts et al , ; Kowalczewski et al , ). The samples were collected after intestinal digestion.…”

Pea Protein for Hempseed Oil Nanoemulsion Stabilization

“…Thus, it is necessary to find consumer-friendly plant proteins to replace those from allergenic ones. Still, some of the valuable proteins sources, such as those obtained from the fraction of potato juice, have not been frequently used [11]. The limitations are related to the unsolved technological problems.…”

Comprehensive studies on the properties of apple juice treated by non-thermal atmospheric plasma in a flow-through system

“…Similar results were previously reported for AJ exposed to UV action, however, in these studies UV-treated AJ revealed no cytotoxic effect on normal intestinal cells but exhibited the significantly induced inhibitory effect on the growth of human colon cancer cells 35 . On the other hand, the data published by Kowalczewski et al 36 indicated that pure potato juice exhibited selective cytotoxic activity against cancer and normal colon cells.…”