The nutritive value of rumen micro-organisms in ruminants

Storm, E; Ørskov, E. R.; Smart, R. I.

doi:10.1079/bjn19830115

Cited by 55 publications

(23 citation statements)

References 14 publications

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“…During the entire experimental period of approximately 10 weeks, the lambs were entirely nourished by continuous intragastric infusion of VFA and minerals intraruminally and various amounts of isolated RMO intra-abomasally (Storm et al 1983). The composition, preparation and administration of the various infusates and other details of the infusion regimen were as previously described (Storm et al 1983).…”

Section: O R S K O Vmentioning

confidence: 99%

“…Analyses of AA, DNA, RNA and PEG were carried out on composite samples of the total ileal material obtained from each animal in each period. Methods of analyses were as described by Storm & Orskov (1983).…”

Section: O R S K O Vmentioning

confidence: 99%

“…The composition, preparation and administration of the various infusates and other details of the infusion regimen were as previously described (Storm et al 1983).…”

Section: O R S K O Vmentioning

confidence: 99%

“…The energy level maintained by rumen VFA infusion alone was 540 kJ/kg live eight^"^ (W0.75) per d, which was estimated to provide 1.25 times the energy requirement for maintenance (0rskov et al 1979). The freeze-dried RMO were isolated as described by Storm & Orskov (1983). RMO was infused abomasally as the only N source in amounts of 140,100,60,20 and 0 g freeze-dried material/d, together with the indigestible marker polyethylene glycol 4000 (PEG) at 10 g/kg RMO.…”

Section: O R S K O Vmentioning

confidence: 99%

“…The present paper describes the estimation by regression analysis of the true digestibilities of total N, total AA-N, RNA, DNA and nineteen individual AA of RMO and the endogenous losses of these substances in the small intestine of three sheep sustained entirely by a constant intraruminal infusion of volatile fatty acids (VFA) and minerals and abomasal infusion of five different amounts of RMO. A preliminary account of some of this work has been given previously (Storm & Orskov, 1982). …”

mentioning

confidence: 99%

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The nutritive value of rumen micro-organisms in ruminants

Storm¹,

Brown²,

Ørskov³

1983

Br J Nutr

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1. An experiment was conducted with three sheep maintained entirely by intragastric nutrition to estimate the digestibility of isolated individual constituents and amino acids (AA) of rumen micro-organisms (RMO) in the small intestine.2. Five levels of RMO were infused into the abomasum. The apparent and true disappearance of the individual components were measured by regression of abomasal input on the passage at the ileum. In order to proceed further to state the AA requirement of ruminants precisely it is also necessary to estimate the digestibility of each AA from RMO since this is the main protein source for ruminants. The present paper describes the estimation by regression analysis of the true digestibilities of total N, total AA-N, RNA, DNA and nineteen individual AA of RMO and the endogenous losses of these substances in the small intestine of three sheep sustained entirely by a constant intraruminal infusion of volatile fatty acids (VFA) and minerals and abomasal infusion of five different amounts of RMO. A preliminary account of some of this work has been given previously (Storm & Orskov, 1982). MATERIALS A N D METHODS Animals.Three Suffolk x (Finnish Landrace x Dorset Horn) wether lambs, weighing between 27 and 36 kg, were used.Each animal was surgically prepared with a permanent rumen cannula and an abomasal catheter, as previously described by Orskov et al. (1979). Furthermore, the animals were fitted with a simple T-piece cannula in the distal part of the ileum, 15-20 mm anterior to the ileo-caecal junction. The open end of the ileal cannula was exteriorized midway up on the right flank of the animal and a plastic loop arranged on each side of the cannulas according to Hecker (1974) so that total collection of ileal fluid was possible.Infusion treatment. After the lambs had been fitted with the cannulas they were kept in individual metabolism cages for 2 weeks before intragastric infusion was commenced. The infusion regimen was then gradually introduced over 10-12 d. Clean drinking water was freely available at all times. 16-2

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Section: O R S K O Vmentioning

confidence: 99%

Section: O R S K O Vmentioning

confidence: 99%

“…The composition, preparation and administration of the various infusates and other details of the infusion regimen were as previously described (Storm et al 1983).…”

Section: O R S K O Vmentioning

confidence: 99%

Section: O R S K O Vmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The nutritive value of rumen micro-organisms in ruminants

Storm¹,

Brown²,

Ørskov³

1983

Br J Nutr

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The nutritive values of feed proteins and feed protein residues resistant to degradation by rumen microorganisms

Rooke

1985

J Sci Food Agric

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Fishmeal (FM), meat and bone meal (MBM) and rapeseed meal (RSM) were incubated for 8 h (RSM) or 24 h (FM, MBM) in nylon bags in the rumens of cattle, in order to prepare from each meal sufficient residues resistant to rumen degradation for evaluation of their nutritive value. The nitrogen (N) and organic matter (OM) contents of FM and MBM were significantly ( R 0 . 0 5 ) reduced by rumen incubation. The modified acid detergent fibre content of RSM was increased, and only traces of glucosinolates remained after rumen incubation. The amino acid profiles of FM and RSM were observed to change as a result of rumen incubation, whereas that of MBM was unchanged. The true N digestibilities of all three protein meals when fed to rats as the sole protein source were significantly (P<0.05) reduced by prior rumen incubation (FM, 0.90 to 0.86; MBM, 0.81 to 0.55 and RSM, U.76 to 0.67). When measured using a rat bioassay, the biological values of FM (65.0 to 79.2) and RSM (53.3 to 83.1) protein were significantly ( R 0 . 0 5 ) increased as a result of rumen incubation but that of MBM was unchanged (47.7 vs 41.1). The N digestibility of the protein meals and of their residues resistant to rumen degradation were also estimated in cattle. Samples of the feedstuffs were placed in small polyester bags, inserted into the proximal duodenum of cattle, recovered in faeces and N disappearance from the bags measured. This technique ranked the N digestibilities of the feedstuffs in the same order as in the rat.

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