The occurrence of circular structures in highly reiterated DNA of the rat

Szala, Stanisław; Chorazy, M; Kilarski, Wincenty

doi:10.1016/0014-5793(71)80573-2

Cited by 6 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the 1970s research topics shifted to issues of cell ultrastructure, structure and function of nucleic acids, repetitive DNA sequences, and chromatin [12][13][14][15][16][17][18][19]. Later studies focused on the mechanisms of DNA transcription and changes in the activity of selected genes in regenerating rat liver and under heat shock conditions [20][21][22].…”

Section: Main Research Topicsmentioning

confidence: 99%

Professor Mieczysław Chorąży and His Works

Lisowska,

Rzeszowska-Wolny,

Sochanik

2024

Postepy Biochem

View full text Add to dashboard Cite

show abstract

Section: Main Research Topicsmentioning

confidence: 99%

Professor Mieczysław Chorąży and His Works

Lisowska,

Rzeszowska-Wolny,

Sochanik

2024

Postepy Biochem

View full text Add to dashboard Cite

show abstract

“…Oda et al (1970) suggested that they were derived from a nuclear satellite DNA. The ability of nuclear (probably satellite) DNA fragments to circularize has been demonstrated (Szala, Chorazy and Kilarski, 1971;Thomas et al, 1970). Thus, any assumed correlation of small circles with malignancy may be the result of greater contamination of mitochondria by the more heterogeneous nuclei of a tumour.…”

Section: Dna Induced Hepatomata In Ratsmentioning

confidence: 99%

An Electron Microscope Study of Mitochondrial DNA in Spontaneous Human Tumours and Chemically Induced Animal Tumours

Kumar¹,

Fox²

1974

Br J Cancer

View full text Add to dashboard Cite

MtDNA was extracted by a phenol method from transplanted and primary DAB induced hepatomata in male Wistar rats, normal rat liver, spontaneous human tumours (2 Wilm's tumours, one neuroblastoma and one adrenal carcinoma), as well as 2 specimens of normal human kidney, BNU induced “leukaemias” in mice and CHO fibroblasts in monolayer culture. The proportion of monomers, catenated dimers and oligomers, open dimers and small circles was determined by electron microscopy of the fractions comprising lower and middle DNA bands in a CsCl-EthBr gradient. Tumours were compared where possible with their normal tissue of origin. Open dimers were found in 2 Wilm's tumours and their attached “normal-looking” kidney tissue but not in normal, non-malignant kidney or any other tissue studied. In Wilm's tumours, the occurrence of open dimers is far from being an all-or-none phenomenon. Malignancy produced little change in the relative proportions of catenated dimers and oligomers in the tissues studied. Small circles were found associated with mtDNA from every tissue. Tumour mtDNA was not more heterogeneous in length than monomers from the corresponding normal tissue, neither was the mean length of tumour mtDNA significantly different from its corresponding normal mtDNA. Images Fig. 4 Fig. 3 Fig. 5

show abstract