The official method for the detection of genetically modified soybeans (German Food Act LMBG § 35): a semi-quantitative study of sensitivity limits with glyphosate-tolerant soybeans (Roundup Ready) and insect-resistant Bt maize (Maximizer)

Jankiewicz, A.; Broll, Hermann; Zagon, Jutta

doi:10.1007/s002170050461

Cited by 48 publications

(43 citation statements)

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“…The sensitivity of PCR reaction was 0.1%. The samples showing the presence of GMOs were analysed using the primers for RR soya (Jankiewicz et al 1999).…”

Section: Samplesmentioning

confidence: 99%

Detection of genetically modified soya, maize, and rice in vegetarian and healthy food products in Serbia

Zdjelar¹,

Nikolić²,

Vasiljević³

et al. 2013

Czech J. Food Sci.

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Zdjelar G., Nikolić Z., Vasiljević I., Bajić B., Jovičić D., Ignjatov M., Milošević D. (2013): Detection of genetically modified soya, maize, and rice in vegetarian and healthy food products in Serbia. Czech J. Food Sci., 31: 43-48.The presence of genetic modifications was analysed in a total of 100 samples of non-labelled vegetarian and healthy food products. The basic raw materials in the samples tested comprised maize, soya, and/or rice. The screening of all samples was performed using the primers for CaMV35S promoter. The positive samples from this initial screening were further subjected to the analysis of specific transgenic material to determine the type of GMO present with subsequent quantification. Roundup Ready soya was found in eight samples, but its content was below the limit of 0.9%. None of the analysed samples of food products contained GM maize and GM rice. Considering that the investigated samples were imported mainly from EU countries, it can be concluded that the control of GMOs is carried out systematically and in accordance with the Serbian GMO Law.

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“…The sensitivity of PCR reaction was 0.1%. The samples showing the presence of GMOs were analysed using the primers for RR soya (Jankiewicz et al 1999).…”

Section: Samplesmentioning

confidence: 99%

Detection of genetically modified soya, maize, and rice in vegetarian and healthy food products in Serbia

Zdjelar¹,

Nikolić²,

Vasiljević³

et al. 2013

Czech J. Food Sci.

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“…In general, DNA can be isolated using various standard protocols like Wizard method [15], CTAB protocol [16], also commercial kits are available in the market for the extraction of DNA. These kit's can be utilized to extract DNA from different food samples like, milk and milk products [17], fermented products [18], oils [19], beverages [20].…”

Section: Different Dna Isolation Methods From Food Productsmentioning

confidence: 99%

DNA as a Biomaterial in Diagnosis of Food Adulteration and Food Safety Assurance

Muthu¹

2017

RDMS

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Apart from general applications of DNA, it can also be used as tool to assure the food safety, which is the main aspect of this review. Increasing GMO productions also increased the concern regarding the adulteration of GMO food products in our daily life. Countries around the world, raising the regulations regarding the GMO products to the consumer's table. Subsequently, the mode of adulteration in food products with GMO products feels difficult to identify. The food products also adulterer with low cost products which can also be detected by the isolation and identification of DNA from that product. The main focus on this review is to discuss the utilization of DNA as an effective tool to detect the adulteration and GMO content in various food products like oil, honey, beverages, baked products, animal feed, fried products, adulteration of milk and milk products. In the field of food safety, microbial contamination is also a major problem. Isolation of DNA from food can also reveals the presence of microbial contamination in the earlier stage. Also, the review emphases the way of utilizing DNA in differentiating organic foods from non-organic foods. ISSN: 2576-8840How to cite this article: Duraimurugan K, Narendhran S, Manikandan M. DNA as a Biomaterial A food should be free from microbial contamination until it reaches the consumers table. All kind of safety measures must be ensured by industries to make it possible. But still microbes are found in food products by any source, leading to the spoilage of food product and causing food borne infections to the consumers. Home cooked food samples collected from the villages in Malawi has shown to be contaminated upto 35% of pathogenic microbes and by further investigation, it was found that contamination of food samples by poor handling and unhygienic conditions [11]. Microbial contamination is a crucial problem in various food industries causing major economic losses. Various industries like dairy, poultry industries follow various cutting-edge technologies to reduce the microbial contamination in end product [12,13]. Microbial contamination can be explored by various physical methods including microscopic and culture depend approaches. But these techniques have various limitations in the confirmation of microbial presence. In present decades, PCR based methods are highly adopted due to its high accuracy and reliability [14].In the present review, we discuss the utilization of DNA as an effective tool in ensuring the quality and safety in various foods and processed food products (Figure 1). DiscussionDifferent DNA isolation methods from food products [20]. Apart from the standard protocols and commercial kits, various modified protocols are also adopted by researchers in order to enhance the efficiency of quality and quantity of extracted DNA [21]. DNA based detection of adulteration and GMO in food productsMilk and milk products: Milk stands as a source of high protein based food for vegetarians around the world. Commercial milk is available in the f...

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“…The absolute limit was the lowest number of initial template copies that could be detected and quantified. Generally, LOD and LOQ were determined using the PCR detection of a dilution series, with absolute detection limits being lower than quantification limits (Hupfer et al, 1998;Jankiewicz et al, 1999).…”

Section: Determination Of Lod and Loq Analysesmentioning

confidence: 99%

Methodology Event-specific qualitative and quantitative PCR detection of the GMO carnation (Dianthus caryophyllus) variety Moonlite based upon the 5'-transgene integration sequence

Jia²,

Jiang³

et al. 2012

Genet. Mol. Res.

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ABSTRACT.To ensure the implementation of genetically modified organism (GMO)-labeling regulations, an event-specific detection method was developed based on the junction sequence of an exogenous integrant in the transgenic carnation variety Moonlite. The 5ꞌ-transgene integration sequence was isolated by thermal asymmetric interlaced PCR. Based upon the 5ꞌ-transgene integration sequence, the event-specific primers and TaqMan probe were designed to amplify the fragments, which spanned the exogenous DNA and carnation genomic DNA. Qualitative and quantitative PCR assays were developed employing the designed primers and probe. The detection limit of the qualitative PCR assay was 0.05% for Moonlite in 100 ng total carnation genomic DNA, corresponding to about 79 copies of the carnation haploid genome; the limit of detection and quantification of the quantitative PCR assay were estimated to be 38 and 190 copies of haploid carnation genomic DNA, respectively. Carnation samples with different contents of genetically modified components were quantified and the bias between the observed and true values of three samples were lower than the acceptance criterion (<25%) of the GMO detection method. These results indicated that these event-specific methods would be useful for the identification and quantification of the GMO carnation Moonlite.

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The official method for the detection of genetically modified soybeans (German Food Act LMBG § 35): a semi-quantitative study of sensitivity limits with glyphosate-tolerant soybeans (Roundup Ready) and insect-resistant Bt maize (Maximizer)

Cited by 48 publications

References 0 publications

Detection of genetically modified soya, maize, and rice in vegetarian and healthy food products in Serbia

Detection of genetically modified soya, maize, and rice in vegetarian and healthy food products in Serbia

DNA as a Biomaterial in Diagnosis of Food Adulteration and Food Safety Assurance

Methodology Event-specific qualitative and quantitative PCR detection of the GMO carnation (Dianthus caryophyllus) variety Moonlite based upon the 5'-transgene integration sequence

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