The oophorectomy effect on Walker 256 tumor inoculated into the vagina and uterine cervix of female rats

Abstract

Purpose: Verify the effect of oophorectomy on the evolution of the Walker 256 tumor inoculated into the vagina and cervix of female rats. Methods: Ten Wistar, female rats were used, distributed into two groups with 05 animals each: Tumor group (TG): Rats inoculated with Walker 256 tumor; Oophorectomy group (OG): oophorectomized rats inoculated with Walker 256 tumor. The day before the tumor vaginal inoculation, acetic acid was inoculated into the vaginas of both groups of rats; the following day, the vaginal walls were scarified with an endocervix brush, and then Walker 256 tumor was inoculated. After 12 days, the tumor was removed together with the vagina and uterine horns for macro and microscopic analyses. The data were submitted to statistical analyses. Results: There was no statistical difference between the two groups; however it was observed that the behavior of tumor growth on the OG group presented greater invasion, compromising the uterine horns. Conclusion: The results of the study on the GO group presented a macroscopic behavior different from the TG group, however, both of them presented similar development in terms of tumor mass. Keywords: Ovariectomy. Cervix Uteri. Vagina. Carcinoma 256, Walker. Rats. RESUMOObjetivo: Verificar o efeito da ooforectomia à inoculação do tumor de Walker 256 em vagina e colo de útero de ratas. Métodos: Foram utilizadas 10 ratas Wistar, fêmeas, virgens, adultas, distribuídas em dois grupos de estudo com 05 animais cada: grupo tumor (GT): ratas inoculadas com tumor de Walker 256, e grupo Ooforectomia (GO): ratas ooforectomizadas e inoculadas com tumor de Walker 256. No dia anterior à inoculação vaginal do tumor, foram inoculados 0,3ml de ácido acético na vagina das ratas de ambos os grupos; no dia seguinte, foi realizada a escarificação da parede vaginal com uma escova de endocérvice e inoculado tumor de Walker 256. Após 12 dias, foi removido o tumor em bloco com vagina e cornos uterinos para análise macro e microscópica. Os dados foram submetidos à análise estatística. Resultados: Não houve diferença estatística entre os dois grupos; no entanto, notou-se que o comportamento de crescimento do tumor no grupo GO apresentou maior invasividade em extensão, comprometendo cornos uterinos. Conclusão: Observou-se que o grupo GO apresentou comportamento macroscópico diferente ao grupo GT, no entanto, em termos totais de massa tumoral ambos apresentam desenvolvimento similar. Descritores: Ovariectomia. Colo do Útero. Vagina. Carcinoma 256 Walker. Rats.

Influence of oophorectomy on glycemia and lipidogram

“…Cholesterol total was maintained at similar levels in all groups (p>0.05), however, its fractions showed a decrease of HDL and increase of LDL and VLDL, mainly from the group 6 (50 days). Wellknown that after oophorectomy, sex hormones circulate in the body at a high level for 21-30 days 11,12 . Therefore, groups 2 to 5 were in adaptation to new condition of hormonal suppression, explaining fluctuations of serum levels identified in this period (30-45 days).…”

“…All surgical procedures were performed in anesthesia (ketamine and xylazine 70 and 10 mg/kg, respectively, IP). The bilateral oophorectomy was performed similar to described by Brito et al 12 . At the end of the following period, 5ml of blood was collected from vena cava for glucose, cholesterol fractions and triglyceride studies, using colorimetric assay, using kits from Labtest ® .…”

Healing incisional surgical wounds using Rose Hip oil in rats

“…After 21 days, the female rats were subjected to exfoliative vaginal cytology, then, the content was deposited on the surface of a glass dish, which was immersed into absolute alcohol for fixation. The glass dishes were stained by Shorr (1941) [8] method and then analyzed using an optical microscope in order to characterize the estral cycle, to confirm anoestrus [9].…”

Avaliação dos efeitos do azul de metileno fotoativado no modelo experimental do Tumor de Walker 256.

“…O carcinossarcoma 256 de Walker foi descoberto em 1928 no laboratório de George Walker do John Hopkins University School of Medicine a partir do desenvolvimento espontâneo de um adenocarcinoma de mama em rata, que por sua vez é mantido em vários laboratórios, por sucessivas inoculações (BRITO et al, 2009;DORNELAS et al, 2006). É um dos poucos modelos de tumores, disponíveis para estudos experimentais nas áreas médicas e biológicas em diferentes linhas de pesquisa (BEVILACQUA et al, 1984;BLACK et al, 1994;EARLE, 1935;GUAITANI et al, 1982;HURTADO et al, 1991;KLULBES et al, 1987;MOTA;REZKALLAH-IWASSO, 1981;MUND et al, 2007;GUASTALE, 1992).…”