The optical biomedical sensors for DNA detection and imaging based on two-photon excited luminescent styryl dyes: phototoxic influence on the DNA

Yashchuk, Valeriy M.; Kudrya, Vladislav Yu.; Losytskyy, Mykhaylo Yu.; Tokar, V. P.; Yarmoluk, S. M.; Dmytruk, I. M.; Prokopets, Vadym M.; Kovalska, Vladyslava; Balanda, A.O.; Kryvorotenko, D. V.; Ogul’chansky, Tymish Yu.

doi:10.1117/12.778096

Cited by 5 publications

(12 citation statements)

References 29 publications

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“…Spectra were taken in methanol solutions (see S5 for detailed information, ESIw). Similarly to positively charged styryl dyes and the recently reported carbazole-based dicationic salt, 6,7,21 V-MOP2 was found to interact with DNA. The quinolizinium derivative has a reduced fluorescence in tris-HCl buffer at pH = 8 mostly due to formation of nonfluorescent aggregates.…”

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confidence: 71%

“…6,7 They were reported to have quite low two-photon absorption crosssection (s 2 o 5 GM, 1 GM = 10 À50 cm 4 s) but they were shown to be efficient staining agents of nucleic-acid-containing organelles in the cytoplasm. 6,7 A series of relatively simple carbazole containing pyridinium dyes have been recently shown to exhibit a very good contrast in staining mitochondria due to their high s 2 (up to 600 GM in acetonitrile). 8 Charged, water soluble bis(styrylbenzene) derivatives have been prepared with s 2 E 400 GM and an emission quantum yield of 0.2-0.4.…”

mentioning

confidence: 99%

“…Similar staining patterns are observed with other positively charged dyes like Rhodamine 123 and styryl dyes with spermine like linkage. 7,20 Fig. 2b shows colocalization images obtained by simultaneously staining the HEK cells with a plasma membrane selective dye (AF594WGA, in red) and with V-MOP2 (in green).…”

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confidence: 99%

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A V-shaped cationic dye for nonlinear optical bioimaging

et al. 2011

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A symmetric cationic molecule with D-π-A(+)-π-D architecture was synthesized with high two-photon absorption cross-section (σ(2) ≈ 1140 GM). Application as a marker in fluorescence microscopy of living cells revealed its presence inside the cell staining vesicular shape organelles in the cytoplasm. Fluorescence lifetime imaging microscopy shows that it is also able to penetrate within the nucleus.

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A V-shaped cationic dye for nonlinear optical bioimaging

et al. 2011

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“…21 A similar staining pattern is observed in other positively charged dyes such as Rhodamine 123 and styryl dyes with spermine-like linkage. 6,42 On the other hand, increasing the concentration of the dye in the incubation medium leads to a clear penetration of the dye also in the nucleus. Figure 8 compares fluorescence intensity and lifetime images of HEK cells stained with V-MOP2.…”

Section: Resultsmentioning

confidence: 99%

Nonlinear Emission of Quinolizinium-Based Dyes with Application in Fluorescence Lifetime Imaging

et al. 2014

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Charged molecules based on the quinolizinum cation have potential applications as labels in fluorescence imaging in biological media under nonlinear excitation. A systematic study of the linear and nonlinear photophysics of derivatives of the quinolizinum cation substituted by either dimethylaniline or methoxyphenyl electron donors is performed. The effects of donor strength, conjugation length, and symmetry in the two-photon emission efficiency are analyzed in detail. The best performing nonlinear fluorophore, with two-photon absorption cross sections of 1140 GM and an emission quantum yield of 0.22, is characterized by a symmetric D-π-A(+)-π-D architecture based on the methoxyphenyl substituent. Application of this molecule as a fluorescent marker in optical microscopy of living cells revealed that, under favorable conditions, the fluorophore can be localized in the cytoplasmatic compartment of the cell, staining vesicular shape organelles. At higher dye concentrations and longer staining times, the fluorophore can also penetrate into the nucleus. The nonlinearly excited fluorescence lifetime imaging shows that the fluorophore lifetime is sensitive to its location in the different cell compartments. Using fluorescence lifetime microscopy, a multicolor map of the cell is drafted with a single dye.

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“…But the same cannot be predicted for other more sensitive cell lines. Certain cell lines may be too sensitive for use of patch sensors (Ceroni et al, ; Quaranta et al, ; Yashchuk et al, ). Some cell lines may require special treatment, like for adherent cell lines, the patch needs to be treated with protein to make cells adhere to it (Bambrick et al, ).…”

Section: Introductionmentioning

confidence: 99%

A unique noninvasive approach to monitoring dissolved O₂ and CO₂ in cell culture

Chatterjee

Uplekar

et al. 2014

Biotech & Bioengineering

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Although online monitoring of dissolved oxygen (DO) and carbon dioxide (DCO2 ) is highly desirable in bioprocesses, small-scale bioreactors are usually not monitored due to the lack of suitable sensors. Traditional electrochemical sensors are usually not used because they are bulky and invasive. Disposable optical sensors are small and only partially invasive, but there are concerns regarding the toxicity of the patch and the phototoxicity of the illuminating light. Here we present a novel, noninvasive, rate-based technique for monitoring DO and DCO2 in cell cultures. A silicone sampling loop which allowed the diffusion of O2 and CO2 through its wall was inserted inside a bioreactor, and then flushed with N2 until the CO2 and O2 inside the loop were completely removed. The gas inside the loop was then allowed to recirculate through gas impermeable tubing to the O2 and CO2 sensors. We have shown that by measuring the initial diffusion rate we were able to determine the partial pressures of the two gases in the culture. The technique could be readily automated and measurements could be made in minutes. It was tested in demonstration experiments by growing murine hybridoma cells in a T-flask and a spinner-flask at 37°C. The results were comparable to those measured with commercially available fluorescence-based patch sensors. These results show that the rate-based method is an effective way to monitor small-scale cell cultures. This measurement mechanism can be easily built into disposable cell culture vessels for facile use.

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The optical biomedical sensors for DNA detection and imaging based on two-photon excited luminescent styryl dyes: phototoxic influence on the DNA

Cited by 5 publications

References 29 publications

A V-shaped cationic dye for nonlinear optical bioimaging

A V-shaped cationic dye for nonlinear optical bioimaging

Nonlinear Emission of Quinolizinium-Based Dyes with Application in Fluorescence Lifetime Imaging

A unique noninvasive approach to monitoring dissolved O₂ and CO₂ in cell culture

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The optical biomedical sensors for DNA detection and imaging based on two-photon excited luminescent styryl dyes: phototoxic influence on the DNA

Cited by 5 publications

References 29 publications

A V-shaped cationic dye for nonlinear optical bioimaging

A V-shaped cationic dye for nonlinear optical bioimaging

Nonlinear Emission of Quinolizinium-Based Dyes with Application in Fluorescence Lifetime Imaging

A unique noninvasive approach to monitoring dissolved O2 and CO2 in cell culture

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Product

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A unique noninvasive approach to monitoring dissolved O₂ and CO₂ in cell culture