The organic coating unit, an all-in-one system for reproducible generation of secondary organic matter aerosol

Keller, A.; Kalbermatter, Daniel M.; Wolfer, Kate; Specht, Patrik; Steigmeier, Peter; Resch, Julian; Kalberer, Markus; Hammer, Tobias; Vasilatou, Konstantina

doi:10.1080/02786826.2022.2110448

Cited by 14 publications

(11 citation statements)

References 29 publications

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“…Even though the angles between our spectra and the reference are around 36° (Table S4), they may still be considered as similar, given that we compare experiments performed under different conditions (both in terms of SOM production as well as sample collection) and by using different mass spectrometers. In addition, similarity to literature spectra has been confirmed by LC-MS measurements using the automated version of our particle generation system …”

Section: Resultssupporting

confidence: 58%

“…In addition, similarity to literature spectra has been confirmed by LC-MS measurements using the automated version of our particle generation system. 33 …”

Section: Resultsmentioning

confidence: 99%

“…The UVA lamp located above the second quartz chamber in the MSC was switched off. The setup for coating a particle with SOM has been automated and miniaturized after this study through a novel system called an organic coating unit (OCU 33 ).…”

Section: Methodsmentioning

confidence: 99%

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Role of Secondary Organic Matter on Soot Particle Toxicity in Reconstituted Human Bronchial Epithelia Exposed at the Air–Liquid Interface

Leni

Ess

Keller

et al. 2022

Environ. Sci. Technol.

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Secondary organic matter (SOM) formed from gaseous precursors constitutes a major mass fraction of fine particulate matter. However, there is only limited evidence on its toxicological impact. In this study, air−liquid interface cultures of human bronchial epithelia were exposed to different series of fresh and aged soot particles generated by a miniCAST burner combined with a micro smog chamber (MSC). Soot cores with geometric mean mobility diameters of 30 and 90 nm were coated with increasing amounts of SOM, generated from the photo-oxidation of mesitylene and ozonolysis of α-pinene. At 24 h after exposure, the release of lactate dehydrogenase (LDH), indicating cell membrane damage, was measured and proteome analysis, i.e. the release of 102 cytokines and chemokines to assess the inflammatory response, was performed. The data indicate that the presence of the SOM coating and its bioavailability play an important role in cytotoxicity. In particular, LDH release increased with increasing SOM mass/total particle mass ratio, but only when SOM had condensed on the outer surface of the soot cores. Proteome analysis provided further evidence for substantial interference of coated particles with essential properties of the respiratory epithelium as a barrier as well as affecting cell remodeling and inflammatory activity.

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Section: Resultssupporting

confidence: 58%

“…In addition, similarity to literature spectra has been confirmed by LC-MS measurements using the automated version of our particle generation system. 33 …”

Section: Resultsmentioning

confidence: 99%

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Role of Secondary Organic Matter on Soot Particle Toxicity in Reconstituted Human Bronchial Epithelia Exposed at the Air–Liquid Interface

Leni

Ess

Keller

et al. 2022

Environ. Sci. Technol.

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“…Keller et al, 2022, showed that the OCU produces SOA, even at high masses, with an atmospherically relevant, realistic chemical composition when compared to conditions in the low µg/m 3 particle mass range (Keller et al, 2022). In the OCU, an organic precursor is oxidized in a small flow chamber by OH and ozone under controlled relative humidity (65%) and temperature conditions (fully described by Keller et al, 2022) (Keller et al, 2022). 155…”

Section: Offline Measurements 145mentioning

confidence: 99%

“…SOA produced from OH-initiated photooxidation of α-pinene is commonly used as an atmospherically representative SOA (Campbell et al, 2019b). High mass concentration SOA (approximately 80 mg/m 3 ) was produced using the Organic Coating Unit (OCU) (Keller et al, 2022) collected on quartz filters for 100 seconds. These very high aerosol concentrations were necessary to minimize the time between 150 particle generation and analysis and therefore reduce the decay of short-lived OP-active aerosol components.…”

Section: Offline Measurements 145mentioning

confidence: 99%

An Automated Online Field Instrument to Quantify the Oxidative Potential of Aerosol Particles via Ascorbic Acid Oxidation

Utinger

Campbell

Bukowiecki

et al. 2023

Preprint

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Abstract. Large-scale epidemiological studies have consistently shown that exposure to ambient particulate matter (PM) is responsible for a variety of adverse health effects. However, the specific physical and chemical properties of particles that are responsible for observed health effects, as well as the underlying mechanisms of particle toxicity upon exposure, remain largely uncertain. Studies have widely suggested that the oxidative potential (OP) of aerosol particles is a key metric to quantify particle toxicity. OP is defined as the ability of aerosol particle components to produce reactive oxidative species (ROS) and deplete antioxidants in vivo. Traditional methods for measuring OP using acellular assays largely rely on analyzing PM collected in filters offline. This is labor intensive and involves a substantial time delay between particle collection and OP analysis. It therefore likely underestimates particle OP, because many reactive chemical components which are contributing to OP are short-lived and therefore degrade prior to offline analysis. Thus, new techniques are required to provide a robust and rapid quantification of particle OP, capturing the chemistry of oxidizing and short-lived highly reactive aerosol components and their concentration dynamics in the atmosphere. To address these measurement shortcomings, we developed a portable online instrument that directly samples particles into an ascorbic acid-based assay under physiologically relevant conditions of pH 6.8 and 37 °C, providing continuous accurate OP measurements with a high time resolution (5 mins). The instrument runs autonomously for up to three days and has a detection limit of about 5 µg/m3 in an urban environment, which allows the characterization of particle OP even in low-pollution areas.

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Insights Into Formation and Aging of Secondary Organic Aerosol From Oxidation Flow Reactors: A Review

Zhang,

Xu,

Lambe

et al. 2024

Curr Pollution Rep

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The organic coating unit, an all-in-one system for reproducible generation of secondary organic matter aerosol

Cited by 14 publications

References 29 publications

Role of Secondary Organic Matter on Soot Particle Toxicity in Reconstituted Human Bronchial Epithelia Exposed at the Air–Liquid Interface

Role of Secondary Organic Matter on Soot Particle Toxicity in Reconstituted Human Bronchial Epithelia Exposed at the Air–Liquid Interface

An Automated Online Field Instrument to Quantify the Oxidative Potential of Aerosol Particles via Ascorbic Acid Oxidation

Insights Into Formation and Aging of Secondary Organic Aerosol From Oxidation Flow Reactors: A Review

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