The Origin of Macromolecule Ionization by Laser Irradiation (Nobel Lecture)

Tanaka, Kōichi

doi:10.1002/anie.200300585

Cited by 172 publications

(97 citation statements)

References 19 publications

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“…The introduction of 2 ionization techniques, electrospray ionization mass spectrometry 29 and matrix-assisted laser/ desoption/ionization, 30,31 in the late 1980s provided major advances in analytical techniques in biomedical research. MS is a powerful tool with the potential to replace fluorometric, radioactive, and photometric monitoring of certain enzyme assays.…”

Section: Discussionmentioning

confidence: 99%

New Mass Spectrometric Assay for Angiotensin-Converting Enzyme 2 Activity

et al. 2006

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Abstract-A novel assay was developed for evaluation of mouse angiotensin-converting enzyme (ACE) 2 and recombinant human ACE2 (rACE2) activity. Using surface-enhanced laser desorption/ionization time of flight mass spectrometry (MS) with ProteinChip Array technology, ACE1 and ACE2 activity could be measured using natural peptide substrates.Plasma from C57BL/6 mice, kidney from wild-type and ACE2 knockout mice, and rACE2 were used for assay validation. Plasma or tissue extracts were incubated with angiotensin I (Ang I; 1296 m/z) or angiotensin II (Ang II; 1045 m/z). Reaction mixtures were spotted onto the ProteinChips WCX2 and peptides detected using surface-enhanced laser desorption/ionization time of flight MS. MS peaks for the substrates, Ang I and Ang II, and the generated peptides, Ang (1-7) and Ang (1-9), were monitored. The ACE2 inhibitor MLN 4760 (0.01 to 100 mol/L) significantly inhibited rACE2 activity (IC 50 ϭ3 nmol/L). Ang II was preferably cleaved by rACE2 (kmϭ5 mol/L), whereas Ang I was not a good substrate for rACE2. There was no detectable ACE2 activity in plasma. Assay specificity was validated in a model of ACE2 gene deletion. In kidney extract from ACE2-deficient mice, there was no generation of Ang (1-7) from Ang II. However, Ang (1-7) was produced when Ang I was used as a substrate. In conclusion, we developed a specific and sensitive assay for ACE2 activity, which used the natural endogenous peptide substrate Ang II. This approach allows for the rapid screening for ACE2, which has applications in drug testing, high-throughput enzymatic assays, and identification of novel substrates/inhibitors of the renin-angiotensin system. Key Words: angiotensin Ⅲ renin-angiotensin system Ⅲ mice Ⅲ angiotensin converting enzyme T he renin-angiotensin system (RAS) is a key regulator of cardiovascular and renal function, both under physiological and pathological conditions. 1

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Section: Discussionmentioning

confidence: 99%

New Mass Spectrometric Assay for Angiotensin-Converting Enzyme 2 Activity

et al. 2006

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“…Technology for the analysis of intact macromolecules was invented in the 1980s, allowing intact protein analysis. In 1985, Koichi Tanaka described a "soft desorption ionization" method using ultrafine metal powder and glycerol that enabled mass spectrometric analysis of biological macromolecules, for which he was awarded the Nobel Prize in Chemistry (4 ). Around the same time, Franz Hillenkamp and Michael Karas reported soft desorption ionization using an organic compound matrix (5 ), for which the term matrix-assisted laser desorption ionization (MALDI) was coined.…”

Section: History and Development Of Commercial Systemsmentioning

confidence: 99%

MALDI-TOF MS for the Diagnosis of Infectious Diseases

2015

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BACKGROUND:First introduced into clinical microbiology laboratories in Europe, MALDI-TOF MS is being rapidly embraced by laboratories around the globe. Although it has multiple applications, its widespread adoption in clinical microbiology relates to its use as an inexpensive, easy, fast, and accurate method for identification of grown bacteria and fungi based on automated analysis of the mass distribution of bacterial proteins.CONTENT: This review provides a historical perspective on this new technology. Modern applications in the clinical microbiology laboratory are reviewed with a focus on the most recent publications in the field. Identification of aerobic and anaerobic bacteria, mycobacteria, and fungi are discussed, as are applications for testing urine and positive blood culture bottles. The strengths and limitations of MALDI-TOF MS applications in clinical microbiology are also addressed.

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“…Today the main ionisation methods deployed are electrospray ionisation (24) and MALDI (25) . These ion sources are combined with various mass analysers that separate the ions by m/z.…”

Section: Protein Identification By Msmentioning

confidence: 99%

Nutriproteomics: technologies and applications for identification and quantification of biomarkers and ingredients

Sénéchal

Kussmann

2011

Proc. Nutr. Soc.

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Nutrition refers to the process by which a living organism ingests and digests food and uses the nutrients therein for growth, tissue maintenance and all other functions essential to life. Food components interact with our body at molecular, cellular, organ and system level. Nutrients come in complex mixtures, in which the presence and concentration of single compounds as well as their interactions with other compounds and the food matrix influence their bioavailability and bioefficacy. Traditionally, nutrition research mainly concentrated on supplying nutrients of quality to nourish populations and on preventing specific nutrient deficiencies. More recently, it investigates health-related aspects of individual ingredients or of complete diets, in view of health promotion, performance optimisation, disease prevention and risk assessment. This review focuses on proteins and peptides, their role as nutrients and biomarkers and on the technologies developed for their analysis. In the first part of this review, we provide insights into the way proteins are currently characterised and analysed using classical and emerging proteomic approaches. The scope of the second part is to review major applications of proteomics to nutrition, from characterisation of food proteins and peptides, via investigation of health-related food benefits to understanding disease-related mechanisms.

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The Origin of Macromolecule Ionization by Laser Irradiation (Nobel Lecture)

Cited by 172 publications

References 19 publications

New Mass Spectrometric Assay for Angiotensin-Converting Enzyme 2 Activity

New Mass Spectrometric Assay for Angiotensin-Converting Enzyme 2 Activity

MALDI-TOF MS for the Diagnosis of Infectious Diseases

Nutriproteomics: technologies and applications for identification and quantification of biomarkers and ingredients

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